High impact information on Actg2

• Rescue of cardiac alpha-actin-deficient mice by enteric smooth muscle gamma-actin [1].
• Testicular SMGA mRNA was first detected and increased substantially in amount during spermiogenesis in the germ cells, in contrast to the decrease of the cytoplasmic beta- and gamma-actin mRNAs towards the end of spermatogenesis [2].
• The implications of the existence of an SMGA in male germ cells are discussed [2].
• Testicular SMGA mRNA was present in the polysome fractions, indicating that it was translated [2].
• The nucleotide sequence of the untranslated region of the SMGA differed substantially from those of other isotypes of mammalian actins [2].

Biological context of Actg2

• These studies demonstrate the existence of an SMGA in male haploid germ cells [2].
• The amino acid sequence deduced from the postmeiotic actin cDNA sequence was nearly identical to that of a chicken gizzard SMGA, with one amino acid replacement at amino acid 359, where glutamine was substituted for proline [2].
• Overall, the studies suggest that upstream elements between -2.7 kb and -571 bp and elements within intron 1 are required for high levels of SMGA gene expression in an appropriate temporal-spatial fashion [3].
• The mouse smooth muscle gamma actin gene is on chromosome 6 [4].
• MATERIALS AND METHODS: A 13.7 kb mouse smooth muscle gamma-actin promoter fragment was ligated to an EGFP reporter gene and microinjected into male mouse pronuclei [5].

Anatomical context of Actg2

• As a first step toward unraveling the mechanisms underlying smooth muscle development and differentiation, we have examined the tissue-specific and developmental expression patterns of six constructs carrying portions of the murine SMGA gene linked to chloramphenicol acetyltransferase (CAT) in stable lines of transgenic mice [3].
• At the nonpermissive temperature for SV40 large T-antigen, the both level of SMGA mRNA and the number of cells staining for this actin are significantly increased in the presence of progesterone, a process that is similar to the upregulation of SMGA in the myometrium late in pregnancy [6].

Regulatory relationships of Actg2

• Urogenital tract expression of enhanced green fluorescent protein in transgenic mice driven by a smooth muscle gamma-actin promoter [5].

References