Disease relevance of OLFM3

• Latrotoxin receptor (CL1AA) and optimedin were shown to be expressed in the iris and ciliary body, as well as in the ganglion and inner nuclear cell layers of the retina, whereas the rat orthologue of the human HNOEL-iso gene was expressed in the iris and sclera and less actively in the trabecular meshwork, retina, and optic nerve [1].

High impact information on OLFM3

• In the human eye, optimedin is expressed in the retina and the trabecular meshwork [2].
• However, unlike noelin, rat optimedin is also highly expressed in the epithelial cells of the iris and the ciliary body in close proximity to the sites of Myoc expression [2].
• Here we describe the identification of a novel olfactomedin-related gene, named optimedin, located on chromosome 1p21 in humans [2].
• The proximal promoter of the Optimedin A variant contains a putative Pax6 binding site in position -86/-70 [3].
• Pax6 regulation of Optimedin in the eye and brain may directly affect multiple developmental processes, including cell migration and axon growth [3].

Biological context of OLFM3

• CONCLUSIONS: Mammalian Noelin 1 evolved from its precursor, followed by evolution of Noelin 3 and Noelin 2 by gene duplication events [4].

Anatomical context of OLFM3

• To study possible effects of optimedin on the differentiation of neural cells, we produced stably transfected PC12 cell lines expressing optimedin under a tetracycline-inducible promoter [5].
• We suggest that expression of optimedin stimulates the formation of adherent and tight junctions on the cell surface and this may play an important role in the differentiation of the brain and retina through the modulation of cytoskeleton organization, cell-cell adhesion and migration [5].
• Expression of optimedin also changed the organization of the actin cytoskeleton and inhibited neurite outgrowth in NGF-stimulated PC12 cells [5].

Regulatory relationships of OLFM3

• Strikingly, however, Noelin 1 and Noelin 3 were found to be expressed as multiple splice variants while only a single spliced transcript could be identified for Noelin 2 [4].
• Optimedin induces expression of N-cadherin and stimulates aggregation of NGF-stimulated PC12 cells [5].

Other interactions of OLFM3

• In addition to EST profiling, RT- PCR was used to detect transcripts of the olfactomedin-domain proteins latrotoxin receptor Lec3 and optimedin in the TM [6].
• Expression of optimedin induced Ca(2+)-dependent aggregation of NGF-stimulated PC12 cells and this aggregation was blocked by the expression of N-cadherin siRNA [5].
• Cells expressing high levels of optimedin showed higher growth rates and stronger adhesion to the collagen extracellular matrix as compared with control PC12 cells [5].

References