Disease relevance of Art2a

• It will be of interest to determine whether these spontaneous Rt6 gene defects constitute part of the polygenetic contribution to autoimmune disease in these animals [1].

High impact information on Art2a

• Recombinant C57BL/6 Rt6-2 and BALB/c Rt6-1 proteins expressed in COS1 cells exhibited ART activity and were documented to be glycosylphosphatidylinositol-linked membrane proteins [2].
• Characterization of mouse Rt6.1 NAD:arginine ADP-ribosyltransferase [3].
• Rat RT6 proteins, and perhaps mouse Rt6, identify a set of immunoregulatory T lymphocytes [3].
• COS-1 cells transfected with a C57BL/6 Rt6-1 cDNA construct expressed a truncated protein consistent in size with that predicted by the presence of the stop codon [2].
• Predicted amino acid sequences of Rt6-2 were identical in both strains, but there was an in-frame stop codon in the sequence of Rt6-1 in C57BL/6 mice not present in BALB/c mice [2].

Biological context of Art2a

• The nucleotide sequences of the Rt6-1 and Rt6-2 coding regions show 87% sequence identity, the deduced amino acid sequences 79% identity [4].
• The results further show that both mouse Rt6 genes are translated into GPI-anchored cell surface molecules and that Rt6 gene expression is restricted to peripheral lymphoid tissues [4].
• The two mouse Rt6 genes (designated Rt6-1 and Rt6-2) encode similar open reading frames that are disrupted by conserved introns [4].
• The NZW mouse shows several strain-specific restriction fragment length variants in this Rt6 locus and five amino acid substitutions occur in the predicted native Rt6 polypeptide of the NZW mouse relative to the corresponding polypeptides of NZB and BALB/c mice [5].
• Abnormalities in mouse Rt6 mRNA are associated with the expression of autoimmunity [6].

Anatomical context of Art2a

• Mouse T-cell antigens Rt6.1 and Rt6.2 are glycosylphosphatidylinositol-anchored arginine-specific adenosine diphosphate (ADP)-ribosyltransferases [7].
• Our results also suggest that upon the interaction of T-cells expressing Rt6.1 with activated macrophages, the NADase activity of the antigen will be stimulated [8].
• A human homologue of the rodent T cell mono(ADP-ribosyl)transferase RT6 mRNA was identified by a systematic partial sequencing of human testis transcripts [9].

Associations of Art2a with chemical compounds

• Mouse Rt6.1 is a thiol-dependent arginine-specific ADP-ribosyltransferase [7].
• In addition to four cysteine residues conserved among mouse Rt6 and rat RT6 antigens, Rt6.1 has two extra cysteine residues at positions 80 and 201 [7].
• The NADase activity of Rt6.1 observed in the presence of DTT was completely inhibited by N-ethylmaleimide (NEM) [8].
• When Cys-80 and Cys-201 in Rt6.1 were replaced with the corresponding residues of Rt6.2, serine and phenylalanine, respectively, Rt6.1 catalyzed the NADase reaction even in the absence of DTT [8].

Analytical, diagnostic and therapeutic context of Art2a

• RT-PCR analyses reveal that the two Rt6 genes are differentially expressed in distinct inbred mouse strains and that their transcripts are properly processed [4].
• Immunoprecipitation of recombinant Rt6-1 and Rt6-2 with anti-FLAG M2 antibody followed by incubation with [32P]NAD+ leads to rapid and covalent incorporation of radioactivity into the light chain of the M2 antibody [10].
• Southern blot and sequence analyses revealed that NZW mice have suffered a deletion of the Rt6.2 gene while the Rt6-1 gene of BxSB mice has been inactivated by a premature stop codon [1].
• Molecular cloning and characterization of the T-cell mono(ADP-ribosyl)transferase RT6. Relationships to other mADPRTs and possible functions [11].

References