High impact information on Chad

• Since heart defects were not detectable in Df(11)18/Dp(11)18 mice, the haplo-insufficiency of one or more genes located between Mpo and Chad may be responsible for the abnormal cardiovascular phenotype [1].
• The chondroadherin gene was mapped to mouse chromosome 11, near D11Mit14, by single-strand conformation polymorphism linkage analysis [2].
• The mouse chondroadherin gene encompasses 4.1 kb and consists of four exons separated by one large intron of 1929 bp followed by two smaller introns of 247 and 225 bp, respectively [2].
• The mouse chondroadherin gene was isolated from a cosmid genomic library by the use of a rat chondroadherin cDNA probe [2].
• The exon/intron organization of the chondroadherin gene differs from those of the other known genes of the leucine-rich repeat (LRR) family in the extracellular matrix [2].

Biological context of Chad

• In tests with these mAbs, relatively little antigenic variation was found among 24 OLCV isolates from Burkina Faso, Chad, Ghana, Ivory Coast, Nigeria, Oman and Saudi Arabia, each virus isolate reacting with at least five of them [3].

Anatomical context of Chad

• By IHC, immunostaining for Chad was seen in epithelial and endothelial layers of the cornea, as well as in lens, ciliary body, and retina of the adult mouse eye [4].
• In most tissues, Chad expression was associated with either peripheral nerves and/or blood vessels [4].
• Chad was highly expressed in human cornea, brain, and skeletal muscle, and moderately in human retina and lens [4].
• IHC analysis of tissue microarray demonstrated presence of Chad in brain (cerebellum), skeletal and cardiac muscles, lung, gastrointestinal tract, ovary, and cartilage [4].

Analytical, diagnostic and therapeutic context of Chad

• Southern blot analysis of mouse genomic DNA revealed a simple pattern of hybridization indicating a single copy gene for chondroadherin [2].
• RESULTS: RT-PCR analysis showed strong expression of Chad in mouse brain, heart, lung, and embryo [4].
• Using mouse serotyping, the currently accepted method for the identification of spotted fever group rickettsiae, the Chad isolate exhibited a specificity difference of 2 when compared to Astrakhan fever rickettsia and at least 4 when compared with other members of the R. conorii complex [5].

References