Disease relevance of Emb

• By RNA blot analyses, high levels of embigin mRNA were detected in embryonal carcinoma cells with different differentiation potentials, namely F9 and PCC4 cells [1].
• To study its function, an embigin cDNA under the control of beta-actin promoter and Rous sarcoma virus enhancer was introduced into L cells [2].
• To further understand the contribution of I-A to the development of disease in murine lupus, we compared the incidence and/or titers of natural thymocytotoxic autoantibodies (NTAs), autoantibodies to red blood cells, gp70 immune complexes (gp70), antibodies to Sm, and rheumatoid factor in NZB (H-2d), NZB.H-2b and NZB.H-2bm12 mice [3].
• These MoAbs precipitated a 70-kilodalton molecule and did not react with cells positive for the classical Gp70 antigen such as YC-8, LSTRA (H-2d) and RBL-5 (H-2b) lymphomas [4].

High impact information on Emb

• Amino acid sequencing showed that the protein is the rat equivalent of the membrane-spanning mouse teratocarcinoma glycoprotein GP-70, a member of the immunoglobulin superfamily related to basigin (Ozawa, M., Huang, R. P., Furukawa, T. , and Muramatsu, T. (1988) J. Biol. Chem. 263, 3059-3062) [5].
• Thus, one role of embigin appears to be promotion of integrin-mediated cell-substratum adhesion [2].
• Embigin is a glycoprotein belonging to the immunoglobulin superfamily, and is preferentially expressed before 10 days of gestation during mouse embryogenesis [2].
• Embigin is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, which is preferentially expressed in early stages of mouse embryogenesis and enhances integrin-mediated cell-substratum adhesion [6].
• Deletion analysis of the 3.5kb DNA fragment revealed that the region between -125 to +1, containing a single Sp1 binding site, is essential for transcription of the embigin gene [6].

Biological context of Emb

• Embigin was strongly expressed in the endoderm during early postimplantation embryogenesis, and in the somite stage in the gut and visceral endoderm [7].
• From day 10 to 15 of gestation, no embigin signal was detected [7].
• The mouse embigin gene, which we cloned, spanned more than 50kb, in which nine exons were present [6].
• These features are shared by the basigin gene; together with protein sequence homology, our results defined a family in the immunoglobulin superfamily, to which embigin and basigin both belong [6].
• The present study deals with embigin gene expression in adult and embryonic cells [1].

Associations of Emb with chemical compounds

• Basigin and embigin, two related members of the immunoglobulin superfamily, a sialomucin MGC-24 and other glycoproteins were discovered as carriers of developmentally regulated carbohydrate markers [8].

Other interactions of Emb

• Embigin/basigin subgroup of the immunoglobulin superfamily: different modes of expression during mouse embryogenesis and correlated expression with carbohydrate antigenic markers [7].
• Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells [9].

References