Disease relevance of Ifi47

• Thus, LRG-47 and IRG-47 have vital, but distinct roles in immune defense against protozoan and bacterial infections [1].
• The 45/47kDa APA protein (Rv1860) of Mycobacterium tuberculosis was produced by Streptomyces lividans [2].

High impact information on Ifi47

• After infection with L. monocytogenes, LRG-47-deficient mice exhibited a profound loss of resistance, whereas IRG-47-deficient mice exhibited completely normal resistance [1].
• The IRG-47 gene is IFN-gamma induced in B cells and encodes a protein with GTP-binding motifs [3].
• The IRG-47 coding sequence contains all three highly conserved peptide motifs in the appropriate spacing characteristic of guanine nucleotide-binding proteins [3].
• The IFN-gamma induction of IRG-47 mRNA occurs in all pre-B and in most B lineage cell lines examined but is only induced in three cell lines tested from other lineages [3].
• IRG-47 encodes an intracellular protein that contains three conserved protein motifs present in GTP-binding proteins [4].

Biological context of Ifi47

• The IRG-47 minimal promoter has the same functional features and organization as the IFN-gamma-inducible promoters of the unrelated murine GBP G-protein multigene family [5].
• The minimal IFN-gamma-inducible IRG-47 gene promoter is 96 bp long and contains a TATA box and an ISRE motif with an internal IRF-1/IRF-2 motif [5].
• Lbc was identified as transforming gene from human leukemic cells and encodes Rho type guanine nucleotide exchange factor with 47kDa molecular weight [6].

Anatomical context of Ifi47

• Bone marrow macrophages that lacked IGTP or LRG-47 displayed greatly attenuated IFN-gamma-induced inhibition of T. gondii growth, while macrophages that lacked IRG-47 displayed normal inhibition [7].
• The 47kDa Akt substrate associates with phosphodiesterase 3B and regulates its level in adipocytes [8].

Associations of Ifi47 with chemical compounds

• Mice lacking LRG-47 failed to control Mtb replication, unlike those missing the related p47 guanosine triphosphatases IRG-47 or IGTP [9].

Regulatory relationships of Ifi47

• Here we report features controlling the uninduced and IFN-gamma-induced expression of the IRG-47 gene [5].

Other interactions of Ifi47

• Computer search of the GenBank and EMBL databases indicates that this cDNA clone is unique but has 57% sequence identity with IRG-47, which is a mouse gene induced by IFN-gamma in pre-B and B lymphocyte cell lines [4].
• We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes [8].

References