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Gene Review

ALKBH3  -  alkB, alkylation repair homolog 3 (E. coli)

Homo sapiens

Synonyms: ABH3, Alkylated DNA repair protein alkB homolog 3, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, DEPC-1, DEPC1, ...
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High impact information on ALKBH3

  • Analysis of purine contacts by DMS and DEPC interference assays shows that the Ad2 octamer can be divided into two regions: one that is recognized both by the POU domain and the homeo domain in an identical fashion, and one that is only recognized by the POU domain [1].
  • Liposomes were formed from three different phospholipids: 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and 1,2-dierucoyl-sn-glycero-3-phosphocholine (DEPC) [2].
  • Cholesterol gave the strongest increase in lateral tension (approximately sixfold) with respect to the DEPC control, followed by lanosterol (approximately twofold), and ergosterol (1.7-fold) [3].
  • A pure synthetic lecithin bilayer, 1,2-dielaidoyl-sn-3-glycero-phoshatidylcholine (DEPC), and binary mixtures of DEPC with 40 mol % of cholesterol, ergosterol, or lanosterol were studied [3].
  • 3. Diethyl pyrocarbonate (DEPC; 10 mM) treatment of P2X4-injected oocytes had no effect on the pH sensitivity of the ATP-activated current [4].

Biological context of ALKBH3

  • Deprotection of the amino and carboxy termini of 1a-1d, followed by cyclization with DEPC as the coupling reagent, gave the above-mentioned cyclic hexapeptides 22, 24a, 24b, and 25 in good yields (26-57%) [5].
  • Z-DNA forming elements were detected by comparing DEPC reactivity in relaxed circular and supercoiled plasmid DNA [6].
  • The substrate, L-cysteine (4 mM), fully protects the transport protein from DEPC inactivation suggesting that this histidine residue is located in the carrier's substrate binding site [7].

Anatomical context of ALKBH3

  • Lipid-protein interactions were studied using Torpedo californica acetylcholine receptor (AChR) as a model system by reconstituting purified AChR into dielaidoylphosphatidylcholine (DEPC, 18:1 trans-9,10) membranes [8].
  • 1--The mechanism of the vasopressin-induced, facilitated transport across toad urinary bladder was studied by treating the luminal membrane of the epithelium with the following reagents of protein functional groups: NEM (SH groups), SITS (amino groups), EEDQ (carboxylic groups), DEPC (histidine) [9].

Associations of ALKBH3 with chemical compounds

  • Some A-tracts also display a very high DEPC reactivity at the adenine adjacent to the 3'-terminal unreactive adenine [10].
  • In the majority of the A-tracts, all but the 3'-terminal thymine are protected from KMnO4 attack, while DEPC reacts significantly with all but the 3'-terminal adenine of the A-tracts [10].
  • An analysis is presented of the complex anisotropy behavior of trans-parinaric acid in single component DEPC lipid bilayers [11].
  • We have examined the reactivity of B DNA with two chemical probes of DNA structure, potassium permanganate (KMnO4; thymine specific) and diethyl pyrocarbonate (DEPC; purine specific, A greater than G) [10].
  • The final steps involve an amide coupling using DEPC and oxazole synthesis via a oxidation/cyclodehydration process [12].

Analytical, diagnostic and therapeutic context of ALKBH3

  • The ether-linked phosphatidylcholines 1-eicosyl-2-dodecyl-rac-glycero-3-phosphocholine (EDPC) and 1-dodecyl-2-eicosyl-rac-glycero-3-phosphocholine (DEPC) have been investigated by differential scanning calorimetry (DSC) and X-ray diffraction [13].


  1. The oct-1 homeo domain contacts only part of the octamer sequence and full oct-1 DNA-binding activity requires the POU-specific domain. Verrijzer, C.P., Kal, A.J., van der Vliet, P.C. Genes Dev. (1990) [Pubmed]
  2. Dynamic assessment of bilayer thickness by varying phospholipid and hydraphile synthetic channel chain lengths. Weber, M.E., Schlesinger, P.H., Gokel, G.W. J. Am. Chem. Soc. (2005) [Pubmed]
  3. Differences in the modulation of collective membrane motions by ergosterol, lanosterol, and cholesterol: a dynamic light scattering study. Hildenbrand, M.F., Bayerl, T.M. Biophys. J. (2005) [Pubmed]
  4. Mutation of histidine 286 of the human P2X4 purinoceptor removes extracellular pH sensitivity. Clarke, C.E., Benham, C.D., Bridges, A., George, A.R., Meadows, H.J. J. Physiol. (Lond.) (2000) [Pubmed]
  5. Solution-phase synthesis of Aib-containing cyclic hexapeptides. Jeremic, T., Linden, A., Heimgartner, H. Chem. Biodivers. (2004) [Pubmed]
  6. Identification of transcriptionally induced Z-DNA segments in the human c-myc gene. Wölfl, S., Wittig, B., Rich, A. Biochim. Biophys. Acta (1995) [Pubmed]
  7. Evidence for an essential histidine residue located in the binding site of the cysteine-specific lysosomal transport protein. Pisoni, R.L., Velilla, V.Q. Biochim. Biophys. Acta (1995) [Pubmed]
  8. Differential scanning calorimetry and Fourier transform infrared analysis of lipid-protein interactions involving the nicotinic acetylcholine receptor. Bhushan, A., McNamee, M.G. Biochim. Biophys. Acta (1990) [Pubmed]
  9. Effect of reagents of protein functional groups on the ADH-induced urea facilitated transport across toad urinary bladder. Ardizzone, C., Lippe, C. Arch. Int. Physiol. Biochim. (1987) [Pubmed]
  10. Chemical reactivity of potassium permanganate and diethyl pyrocarbonate with B DNA: specific reactivity with short A-tracts. McCarthy, J.G., Williams, L.D., Rich, A. Biochemistry (1990) [Pubmed]
  11. Analysis of the anisotropy decay of trans-parinaric acid in lipid bilayers. Ruggiero, A., Hudson, B. Biophys. J. (1989) [Pubmed]
  12. Total synthesis of the antiviral marine natural product (-)-hennoxazole A. Yokokawa, F., Asano, T., Shioiri, T. Org. Lett. (2000) [Pubmed]
  13. Structure and thermotropic properties of hydrated 1-eicosyl-2-dodecyl-rac-glycero-3-phosphocholine and 1-dodecyl-2-eicosyl-rac-glycero-3-phosphocholine bilayer membranes. Mattai, J., Witzke, N.M., Bittman, R., Shipley, G.G. Biochemistry (1987) [Pubmed]
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