Disease relevance of ARSG

• The high resolution made it possible to distinguish hemoglobin variants such as Bart's, AC, AD, AE, AG, AS, ASG, CC, SC, SS, Winnipeg, Sealy and beta-chain variants with thalassemia such as S/beta +, S/beta 0 and S(C)-beta + thalassemia [1].
• United States Thrombotic Thrombocytopenic Purpura Apheresis Study Group (US TTP ASG): multicenter survey and retrospective analysis of current efficacy of therapeutic plasma exchange [2].

High impact information on ARSG

• Using the ASG banding technique, the exact identification of the abnormal chromosomes was successfully made in 22 aneuploid patients [3].
• Through bioinformatic searches of the EST database, we have identified a novel gene consisting of 11 exons and encoding a 525 aa protein that shares a high degree of sequence similarity with all sulphatases and in particular with arylsulphatases, hence the tentative name Arylsulfatase G (ARSG) [4].
• Tn SGP was 2000-fold more effective as an inhibitor than the mucins (ASG and desialized OSG), which contain a high content of terminal alpha-GalNAc-O-serine (threonine) residues [5].
• Chromosome analyses were carried out on 'blind-coded' slides using the ASG banding technique as the routine stain [6].
• Pronase treatment in Epon sections for 24 h digested ASG alone, whereas in glycol-methacrylate embedded sections, ESG were digested first, ASG were digested thoroughly after 30 min, but ICG were not digested completely after 90 min [7].

Biological context of ARSG

• The ring chromosome was identified by the ASG banding technique [8].
• Changes in elemental composition of human chromosomes during a G-banding (ASG) and a C-banding (BSG) procedure [9].
• Fractions of TBM, solubilized by either dilute alkali (0.01 N NaOH), by reduction and alkylation of the disulfide bonds, or by 0.3 M acetic acid extraction, all contained the antigen and possessed properties similar to those of ASG [10].
• The presence of ER(+) cells in histochemical assay was not related to menopause or to the molecular pattern of ER determined by the ASG method, or to the histological type of cancer [11].

Anatomical context of ARSG

• Fine structural and cytochemical studies were performed to examine the nature of three types of specific granules found in the atrium of lamprey; specific granules of the atrial muscle cell (ASG), interstitial cell granules (ICG) and endocardial endothelial granules (ESG) [7].
• The in vivo study indicated that interactions of ASG secretions and spermatozoa were complicated by the presence of uterine secretions [12].
• At rupture in series ASG (sutured/glued), the suture material was being subjected to a stress of 64.91 MPa, thus confirming the existence of an interaction between the suture and the shear stress exerted by the suture on the samples of pericardium [13].
• The analysis was carried out on short-term cultures of sternal bone marrow with the ASG and fluorescence method [14].

Associations of ARSG with chemical compounds

• Phosphotungstic acid at low pH stained ICG and ASG strongly positive, and ESG weakly positive [7].

Analytical, diagnostic and therapeutic context of ARSG

• Northern blot analysis of Arylsulfatase G revealed a ubiquitous expression pattern [4].
• Twenty large apheresis centers within the United States comprising the US TTP ASG responded to a survey to establish the current status of TPE in TTP [2].
• SDS-PAGE profiles showed that both uterine and ASG secretions could modify proteins on the sperm surface [12].

References