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Gene Review

ura4  -  orotidine 5'-phosphate decarboxylase Ura4

Schizosaccharomyces pombe 972h-

 
 
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High impact information on ura4

  • In a strain carrying replacement of 7.5 kb of the K-region with the ura4 gene, we discovered that ura4 silencing and efficiency of mating-type switching were covariegated and were regulated by an epigenetic mechanism [1].
  • The chromatin structure of the ura4 gene at centromeres was altered, suggesting that the unusual chromatin encroaches into the gene and inhibits normal expression [2].
  • A plasmid bearing the SV2NEO gene, which can confer G418 resistance to mouse cells, was integrated at the ura4 locus on S. pombe chromosome III [3].
  • The weakened centromeric function of pli1Delta cells may be related to the defective heterochromatin structure at the central core, as shown by the reduced silencing of an ura4 variegation reporter gene inserted at cnt and imr [4].
  • Interestingly, pli1Delta cells also exhibit enhanced loss of the ura4 reporter at these loci, likely by gene conversion using homologous sequences as information donors [4].
 

Biological context of ura4

 

Associations of ura4 with chemical compounds

 

Other interactions of ura4

  • These telomeres exert reversible position effects on the expression of the adjacent ura4 or ade6 genes [6].
  • To identify genes involved in the mechanism to ensure ordered 5' to 3' exon joining in constitutively spliced pre-mRNAs, we screened for mutants that cause exon skipping in the fission yeast Schizosaccharomyces pombe using a reporter plasmid, which contains the ura4(+) gene with the nda3 intron 1-exon 2-intron 2 sequence [11].
  • Mutations in the six rec genes tested also caused a decrease in intragenic recombination at the ura4 locus; five of these mutations also reduced intergenic recombination between the pro2 and arg3 genes [12].
  • 1) Use of the strong adh1 promoter to drive the expression of ura4 did not affect the disruption frequency but modestly increased the transformation efficiency [13].
  • We used suc1 as the target gene for disruption and ura4 as the selectable marker [13].
 

Analytical, diagnostic and therapeutic context of ura4

  • In the present study, the S.pombe ura4+ gene was amplified by PCR with oligonucleotides that had short flanking regions ( approximately 40 bp) to the target gene [14].
  • Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker [9].

References

  1. Chromosomal inheritance of epigenetic states in fission yeast during mitosis and meiosis. Grewal, S.I., Klar, A.J. Cell (1996) [Pubmed]
  2. Position effect variegation at fission yeast centromeres. Allshire, R.C., Javerzat, J.P., Redhead, N.J., Cranston, G. Cell (1994) [Pubmed]
  3. A fission yeast chromosome can replicate autonomously in mouse cells. Allshire, R.C., Cranston, G., Gosden, J.R., Maule, J.C., Hastie, N.D., Fantes, P.A. Cell (1987) [Pubmed]
  4. Role of the fission yeast SUMO E3 ligase Pli1p in centromere and telomere maintenance. Xhemalce, B., Seeler, J.S., Thon, G., Dejean, A., Arcangioli, B. EMBO J. (2004) [Pubmed]
  5. Three ARS elements contribute to the ura4 replication origin region in the fission yeast, Schizosaccharomyces pombe. Dubey, D.D., Zhu, J., Carlson, D.L., Sharma, K., Huberman, J.A. EMBO J. (1994) [Pubmed]
  6. Telomere-associated chromosome breakage in fission yeast results in variegated expression of adjacent genes. Nimmo, E.R., Cranston, G., Allshire, R.C. EMBO J. (1994) [Pubmed]
  7. Position- and orientation-independent activity of the Schizosaccharomyces pombe meiotic recombination hot spot M26. Fox, M.E., Virgin, J.B., Metzger, J., Smith, G.R. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  8. Introduction of functional artificial introns into the naturally intronless ura4 gene of Schizosaccharomyces pombe. Gatermann, K.B., Hoffmann, A., Rosenberg, G.H., Käufer, N.F. Mol. Cell. Biol. (1989) [Pubmed]
  9. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Grimm, C., Kohli, J., Murray, J., Maundrell, K. Mol. Gen. Genet. (1988) [Pubmed]
  10. Gene disruption in Schizosaccharomyces pombe using a temperature-sensitive Ura4p. Davis, K., Pateman, C., Davey, J. Yeast (1999) [Pubmed]
  11. Mutations in the SF1-U2AF59-U2AF23 Complex Cause Exon Skipping in Schizosaccharomyces pombe. Haraguchi, N., Andoh, T., Frendewey, D., Tani, T. J. Biol. Chem. (2007) [Pubmed]
  12. Seventeen complementation groups of mutations decreasing meiotic recombination in Schizosaccharomyces pombe. De Veaux, L.C., Hoagland, N.A., Smith, G.R. Genetics (1992) [Pubmed]
  13. A study of integrative transformation in Schizosaccharomyces pombe. Grallert, B., Nurse, P., Patterson, T.E. Mol. Gen. Genet. (1993) [Pubmed]
  14. PCR-mediated direct gene disruption in Schizosaccharomyces pombe. Kaur, R., Ingavale, S.S., Bachhawat, A.K. Nucleic Acids Res. (1997) [Pubmed]
 
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