Disease relevance of cut3

• Taken together, our data suggest that there are mitotic as well as nonmitotic chromosomal abnormalities associated with loss of condensin function in budding yeast [1].

High impact information on cut3

• Chromosome condensation requires condensin, which comprises five subunits [2].
• A definitive defect of interphase is abolishment of Cds1 (a checkpoint kinase) activation in the presence of hydroxyurea in both cnd2-1 mutant cells and in cells where other condensin subunits have been genetically disrupted [2].
• These results imply that condensin is not simply required for compaction, but plays a more complex role in chromosome architecture that is essential for mitotic and meiotic sister chromatid segregation [3].
• To repress gene expression, MIX-1 acts in an X-chromosome complex that resembles the mitotic condensin complex yet lacks chromosome segregation function [3].
• Condensin subunits tagged with GFP were observed to alter dramatically their localization during the cell cycle, enriched in the nucleus during mitosis, but cytoplasmic during other stages [4].

Biological context of cut3

• Plasmids carrying the DNA topoisomerase I gene partly suppressed the temperature sensitive phenotype of cut3-477, suggesting that the cut3 protein might be involved in chromosome DNA topology [5].
• Loss of the cut3 protein by gene disruption fails to maintain the nuclear chromatin architecture even in interphase [5].
• The cut3 protein, having the highest similarity to the mouse protein, is localized in the nucleus throughout the cell cycle [5].
• The cut3 mutant was suppressed by an increase in the cut14+ gene dosage [5].
• Cti1 may have a supporting role for the DNA repair function of condensin [6].

Anatomical context of cut3

• In eukaryotic cells, three essential SMC complexes have been defined: cohesin, condensin, and the Smc5/6 complex [7].

Other interactions of cut3

• Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis [5].
• Neither the phosphorylation of histone H3 that accompanies chromosome condensation nor condensin recruitment to mitotic chromatin were seen when Ark1 function was compromised [8].

Analytical, diagnostic and therapeutic context of cut3

• GST pull-down assay and immunoprecipitation supported physical interaction between Cti1 and condensin [6].

References