High impact information on rad9

• The fission yeast Schizosaccharomyces pombe rad9 gene promotes cell survival through activation of cell cycle checkpoints induced by DNA damage [1].
• In the present study, we have attempted to test the predictions of this model by asking whether the PCNA alignment identifies functionally important residues or explains mutant phenotypes of hus1, rad1, or rad9 alleles [2].
• Cloning and characterisation of the rad9 DNA repair gene from Schizosaccharomyces pombe [3].
• The rad9 gene has been cloned by complementation of the gamma-ray sensitivity of the mutant cell line [3].
• Schizosaccharomyces pombe cells that contain a mutation within rad9 are sensitive to ionizing radiation, UV light and hydroxyurea, relative to wild-type strains [4].

Biological context of rad9

• Extragenic suppressors of Schizosaccharomyces pombe rad9 mutations uncouple radioresistance and hydroxyurea sensitivity from cell cycle checkpoint control [4].
• Results are discussed in terms of models to explain the putative role of rad9 and the suppressor genes in promoting radioresistance and mediating checkpoint controls responsive to DNA damage or incomplete DNA replication [4].
• The suppressor loci also conferred radioresistance upon cells containing rad9-192, which differs from rad9+ by a single base pair change [4].
• DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin [5].
• In contrast, Sz. octosporus rad9 contains five introns and codes for a 48 210-Da protein that is 432-aa long [6].

Other interactions of rad9

• The upstream signal transduction pathways involved in the G2 arrest following ionizing radiation remain obscure in mammalian cells; however, in the budding yeast the rad9 gene and in the fission yeast the chk1/rad27 gene are involved [7].

Analytical, diagnostic and therapeutic context of rad9

• The main form, Hus1-B, participates in a protein complex with Rad9 and Rad1, consistent with reports that Rad1-Hus1 immunoprecipitation is dependent on the rad9(+) locus [8].
• Molecular cloning and analysis of Schizosaccharomyces pombe rad9, a gene involved in DNA repair and mutagenesis [9].

References