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Gene Review

chk1  -  Chk1 protein kinase

Schizosaccharomyces pombe 972h-

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High impact information on chk1


Biological context of chk1

  • We show that strains carrying any of these mutations enter lethal mitosis in the absence of the radiation checkpoint chk1/rad27 [6].
  • The upstream signal transduction pathways involved in the G2 arrest following ionizing radiation remain obscure in mammalian cells; however, in the budding yeast the rad9 gene and in the fission yeast the chk1/rad27 gene are involved [7].
  • Of 44 chk1 mutants recovered, six encode full-length proteins that confer a DNA damage-sensitive phenotype [8].
  • To further characterize the behavior of Chk1 in response to DNA damage, we used PCR-based mutagenesis of the chk1 gene coupled with in vivo gap repair to generate mutant alleles [8].
  • Induction of chk1 enhanced the survival of an A-T cell line in response to radiation exposure as determined by cell viability and reduction of radiation-induced chromosome aberrations [9].

Other interactions of chk1

  • High levels of Spd1p lead to an increase in Y15 phosphorylation of Cdc2p and we show that the block over G(2) requires the Wee1p kinase and is dependent on the rad and chk1/cds1 checkpoint genes [10].


  1. Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2. Walworth, N., Davey, S., Beach, D. Nature (1993) [Pubmed]
  2. Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase. Furnari, B., Rhind, N., Russell, P. Science (1997) [Pubmed]
  3. Recovery from DNA damage checkpoint arrest by PP1-mediated inhibition of Chk1. den Elzen, N.R., O'Connell, M.J. EMBO J. (2004) [Pubmed]
  4. Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation. O'Connell, M.J., Raleigh, J.M., Verkade, H.M., Nurse, P. EMBO J. (1997) [Pubmed]
  5. p56(chk1) protein kinase is required for the DNA replication checkpoint at 37 degrees C in fission yeast. Francesconi, S., Grenon, M., Bouvier, D., Baldacci, G. EMBO J. (1997) [Pubmed]
  6. DNA polymerase delta is required for the replication feedback control of cell cycle progression in Schizosaccharomyces pombe. Francesconi, S., De Recondo, A.M., Baldacci, G. Mol. Gen. Genet. (1995) [Pubmed]
  7. The molecular basis for cell cycle delays following ionizing radiation: a review. Maity, A., McKenna, W.G., Muschel, R.J. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology. (1994) [Pubmed]
  8. A novel genetic screen identifies checkpoint-defective alleles of Schizosaccharomyces pombe chk1. Wan, S., Walworth, N.C. Curr. Genet. (2001) [Pubmed]
  9. Chk1 complements the G2/M checkpoint defect and radiosensitivity of ataxia-telangiectasia cells. Chen, P., Gatei, M., O'Connell, M.J., Khanna, K.K., Bugg, S.J., Hogg, A., Scott, S.P., Hobson, K., Lavin, M.F. Oncogene (1999) [Pubmed]
  10. The Spd1p S phase inhibitor can activate the DNA replication checkpoint pathway in fission yeast. Borgne, A., Nurse, P. J. Cell. Sci. (2000) [Pubmed]
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