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Klp3A  -  Kinesin-like protein at 3A

Drosophila melanogaster

Synonyms: 3A7Kin, BcDNA:LD21815, CG8590, DmKLP3A, DmKlp3A, ...
 
 
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High impact information on Klp3A

  • To further investigate the relationships between the central spindle and the contractile ring, we examined meiosis in the cytokinesis-defective mutants KLP3A and diaphanous and in testes treated with cytochalasin B [1].
  • 17 MMAPs were identified; seven have been previously implicated in cellularization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin [2].
  • The KLP3A protein localizes to the equator of the central spindle during late anaphase and telophase of male meiosis [3].
  • Mutations in the KLP3A gene disrupt the interdigitation of microtubules in spermatocyte central spindles [3].
  • We have also found that the KLP3A protein is located throughout the metaphase spindle during meiosis and the early embryonic mitotic divisions, but later accumulates specifically at the midzone of these same spindles during telophase [4].
 

Biological context of Klp3A

  • Finally, we show that the Klp3A protein localizes within the oocyte nucleus during meiotic prophase, the time at which exchange distribution is established, and extensively colocalizes with DNA [5].
  • We propose that KLP3A acts on MTs associated with chromosome arms and the central spindle to organize ipMT bundles, to drive spindle pole separation and to facilitate chromatid motility [6].
  • Kinetic analysis suggests that KLP3A contributes to spindle pole separation during the prometaphase-to-metaphase transition (when it antagonizes Ncd) and anaphase B, to normal rates of chromatid motility during anaphase A, and to the proper spacing of daughter nuclei during telophase [6].
  • Time-lapse confocal observations of mitosis in living embryos reveal that KLP3A inhibition disrupts the organization of interpolar (ip) MTs and produces short spindles [6].
  • Here we examined the contribution of the chromokinesin, KLP3A, to mitotic spindle morphogenesis and chromosome movements in Drosophila embryos and cultured S2 cells [6].
 

Anatomical context of Klp3A

 

Other interactions of Klp3A

  • Third, Klp3A and Polo, two markers of the wild-type central spindle are also found around the pointed end of the mutant cones [8].
  • In chickadee and KLP3A mutant ana-telophases the cortical anillin band fails to constrict, indicating that its contraction is normally driven by the cytokinetic ring [9].
  • We show that the mei-352 mutation is an allele of the klp3A gene, which encodes a kinesin-like protein of the Kinesin-4 family [5].
 

Analytical, diagnostic and therapeutic context of Klp3A

  • By immunofluorescence, KLP3A associates with nonfibrous punctae that concentrate in nuclei and display MT-dependent associations with spindles [6].

References

  1. Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis. Giansanti, M.G., Bonaccorsi, S., Williams, B., Williams, E.V., Santolamazza, C., Goldberg, M.L., Gatti, M. Genes Dev. (1998) [Pubmed]
  2. Lava lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularization. Sisson, J.C., Field, C., Ventura, R., Royou, A., Sullivan, W. J. Cell Biol. (2000) [Pubmed]
  3. The Drosophila kinesin-like protein KLP3A is a midbody component required for central spindle assembly and initiation of cytokinesis. Williams, B.C., Riedy, M.F., Williams, E.V., Gatti, M., Goldberg, M.L. J. Cell Biol. (1995) [Pubmed]
  4. The Drosophila kinesin-like protein KLP3A is required for proper behavior of male and female pronuclei at fertilization. Williams, B.C., Dernburg, A.F., Puro, J., Nokkala, S., Goldberg, M.L. Development (1997) [Pubmed]
  5. The Drosophila meiotic mutant mei-352 is an allele of klp3A and reveals a role for a kinesin-like protein in crossover distribution. Page, S.L., Hawley, R.S. Genetics (2005) [Pubmed]
  6. The chromokinesin, KLP3A, dives mitotic spindle pole separation during prometaphase and anaphase and facilitates chromatid motility. Kwon, M., Morales-Mulia, S., Brust-Mascher, I., Rogers, G.C., Sharp, D.J., Scholey, J.M. Mol. Biol. Cell (2004) [Pubmed]
  7. Klp67A destabilises pre-anaphase microtubules but subsequently is required to stabilise the central spindle. Gatt, M.K., Savoian, M.S., Riparbelli, M.G., Massarelli, C., Callaini, G., Glover, D.M. J. Cell. Sci. (2005) [Pubmed]
  8. Organized microtubule arrays in gamma-tubulin-depleted Drosophila spermatocytes. Sampaio, P., Rebollo, E., Varmark, H., Sunkel, C.E., González, C. Curr. Biol. (2001) [Pubmed]
  9. The role of anillin in meiotic cytokinesis of Drosophila males. Giansanti, M.G., Bonaccorsi, S., Gatti, M. J. Cell. Sci. (1999) [Pubmed]
 
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