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Gene Review

IGSF3  -  immunoglobulin superfamily, member 3

Homo sapiens

Synonyms: EWI-3, EWI3, Glu-Trp-Ile EWI motif-containing protein 3, IgSF3, Immunoglobulin superfamily member 3, ...
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Disease relevance of IGSF3

  • Both tryptic and Staphylococcus aureus V8 protease peptide analyses showed homology between p168 and the beta- and alpha-chains and between p125 and the alpha- and gamma-chains [1].

High impact information on IGSF3

  • Direct amino acid sequencing of a V8 protease peptide from hnRNP core protein A1 showed that this peptide contained at its amino terminus the last 11 amino acids of UP I followed by 19 amino acids which are encoded by the open reading frame of cDNA clone pRP10 immediately following the UP I sequence [2].
  • Staphylococcal V8 peptide mapping indicated that proteins of similar molecular weights were highly homologous to each other across cell lines, despite the diverse hematopoietic lineages of these cells and the genetic heterogeneity of the patients from whom the CML cell lines were derived [3].
  • Limit digest peptide fragments, generated by prolonged exposure of the labeled receptor to trypsin, cyanogen bromide, or Staphylococcus aureus V8 protease, were purified to homogeneity by high performance liquid chromatography (HPLC), and the position of the labeled residue was determined by sequential Edman degradation [4].
  • The catalytic subunit alpha and the beta subunit of phosphatases I, III, and IV displayed identical V8 and papain peptide maps, respectively, while the peptide maps of the alpha, beta, gamma, and delta subunits were clearly distinct [5].
  • V8 protease peptide mapping revealed structural differences between the T cell receptor alpha-chain isolated from HPB-ALL on one hand and from the normal 3D6+ lines on the other, whereas the beta-chains did not differ greatly in primary structure according to this analysis [6].

Biological context of IGSF3

  • We report the isolation and characterization of a novel cDNA IGSF3 that contains a 3648-bp open reading frame encoding an apparent immunoglobulin (Ig)-like membrane protein characterized by eight Ig domains [7].
  • Molecular cloning of a human cDNA IGSF3 encoding an immunoglobulin-like membrane protein: expression and mapping to chromosome band 1p13 [7].
  • A common immunoreactive V8 protease peptide of approx. 17 kDa has been identified in B2 and mBx hnRNP polypeptides. mBx protein species are identified in cells of murine origin, and have a ubiquitous tissue distribution and developmental appearance [8].
  • Analysis of proteins from these RTK-expressing cells revealed that a Mr 85,000 protein showed in vitro phosphorylation, and V8 protease peptide mapping showed that this protein was p85, the regulatory subunit of phosphatidylinositol 3-kinase (PI3-kinase) [9].
  • These results were confirmed by sequence determination of a variant V8 peptide in the case of Trieste, and by DNA sequence analysis for the other two variants [10].

Anatomical context of IGSF3

  • IGSF3 has an overall structural similarity and strong sequence similarity to V7, a human leukocyte surface protein [7].
  • The Mr approximately 42,000 component was homologous to rabbit skeletal muscle actin by peptide mapping with staphylococcal V-8 protease [11].
  • C' and A' produced V8- and papain-peptide maps identical to those of the 34 kDa catalytic C and the 63 kDa regulatory A subunits of the Mn2+-independent conventional protein phosphatase in human erythrocyte cytosol, respectively [12].

Associations of IGSF3 with chemical compounds

  • With both aziridines, the labeled residue was at position 1 in the tryptic peptide, position 2 in the cyanogen bromide peptide, and position 7 in the V8 protease peptide [4].
  • Sequence analysis of the abnormal V8 peptide revealed that the variant arises from a previously unreported substitution at position 505 where glutamic acid has been replaced by lysine [13].

Analytical, diagnostic and therapeutic context of IGSF3

  • 3D6 reactive T cell receptors isolated from HPB-ALL and normal cell lines were analyzed biochemically by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, isoelectric focusing, and V8 protease peptide mapping [6].
  • V8 protease catalyzed a chain of ligation steps between pH 6 and 8 at 4 degrees C, producing a gamut of covalent oligomers (dimer through octamer or higher) of a native protein segment TAAAKFE (S39) derived from ribonuclease A (RNAse A) [14].
  • Further, the study projects the presence of considerable innate synthetic potential in V8 protease, baring rich possibilities of protein engineering of this enzyme to generate a "V8 peptide ligase."[14]
  • This fragment was isolated on a preparative scale by reversed-phase HPLC and subjected to V8 proteinase digestion [13].


  1. Identification and structural characterization of two incompletely processed forms of the fourth component of human complement. Chan, A.C., Atkinson, J.P. J. Clin. Invest. (1983) [Pubmed]
  2. Mammalian single-stranded DNA binding protein UP I is derived from the hnRNP core protein A1. Riva, S., Morandi, C., Tsoulfas, P., Pandolfo, M., Biamonti, G., Merrill, B., Williams, K.R., Multhaup, G., Beyreuther, K., Werr, H. EMBO J. (1986) [Pubmed]
  3. Cell lines and peripheral blood leukocytes derived from individuals with chronic myelogenous leukemia display virtually identical proteins phosphorylated on tyrosine residues. Huhn, R.D., Posner, M.R., Rayter, S.I., Foulkes, J.G., Frackelton, A.R. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  4. Identification of cysteine 530 as the covalent attachment site of an affinity-labeling estrogen (ketononestrol aziridine) and antiestrogen (tamoxifen aziridine) in the human estrogen receptor. Harlow, K.W., Smith, D.N., Katzenellenbogen, J.A., Greene, G.L., Katzenellenbogen, B.S. J. Biol. Chem. (1989) [Pubmed]
  5. Three distinct forms of type 2A protein phosphatase in human erythrocyte cytosol. Usui, H., Imazu, M., Maeta, K., Tsukamoto, H., Azuma, K., Takeda, M. J. Biol. Chem. (1988) [Pubmed]
  6. Human T cell lines differing in phenotype and specificity are reactive with the same anti-idiotypic antibody. Borst, J., Boylston, A.W., de Vries, J.E., Spits, H. J. Immunol. (1986) [Pubmed]
  7. Molecular cloning of a human cDNA IGSF3 encoding an immunoglobulin-like membrane protein: expression and mapping to chromosome band 1p13. Saupe, S., Roizès, G., Peter, M., Boyle, S., Gardiner, K., De Sario, A. Genomics (1998) [Pubmed]
  8. Recognition of subsets of the mammalian A/B-type core heterogeneous nuclear ribonucleoprotein polypeptides by novel autoantibodies. Dangli, A., Plomaritoglou, A., Boutou, E., Vassiliadou, N., Moutsopoulos, H.M., Guialis, A. Biochem. J. (1996) [Pubmed]
  9. Activation of phosphatidylinositol 3-kinase is necessary for differentiation of FDC-P1 cells following stimulation of type III receptor tyrosine kinases. Kubota, Y., Angelotti, T., Niederfellner, G., Herbst, R., Ullrich, A. Cell Growth Differ. (1998) [Pubmed]
  10. Structural characterization of three genetic variants of human serum albumin modified in subdomains IIB and IIIA. Galliano, M., Watkins, S., Madison, J., Putnam, F.W., Kragh-Hansen, U., Cesati, R., Minchiotti, L. Eur. J. Biochem. (1998) [Pubmed]
  11. Structural analysis of the myeloma-associated membrane antigen KMA. Goodnow, C.C., Raison, R.L. J. Immunol. (1985) [Pubmed]
  12. Direct metal analyses of Mn2+-dependent and -independent protein phosphatase 2A from human erythrocytes detect zinc and iron only in the Mn2+-independent one. Nishito, Y., Usui, H., Shinzawa-Itoh, K., Inoue, R., Tanabe, O., Nagase, T., Murakami, T., Takeda, M. FEBS Lett. (1999) [Pubmed]
  13. Protein and DNA sequence analysis of a 'private' genetic variant: albumin Ortonovo (Glu-505-->Lys). Galliano, M., Minchiotti, L., Iadarola, P., Stoppini, M., Giagnoni, P., Watkins, S., Madison, J., Putnam, F.W. Biochim. Biophys. Acta (1993) [Pubmed]
  14. An enigmatic peptide ligation reaction: protease-catalyzed oligomerization of a native protein segment in neat aqueous solution. Kumaran, S., Datta, D., Roy, R.P. Protein Sci. (2000) [Pubmed]
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