Disease relevance of THBS2

• No significant differences in TSP1 and TSP2 expressions were found between control adrenals and nonfunctional adenomas [1].

High impact information on THBS2

• CISP differs greatly, however, from TSP1 by the inducibility of its synthesis by cAMP [2].
• We report here the characterization of the molecular structure of CISP [2].
• Inhibition of sulfation by chlorate did not prevent CISP secretion, whereas inhibition of glycosylation by tunicamycin blocked it [2].
• Analysis of CISP by polyacrylamide gel electrophoresis in the absence or presence of SDS indicated an apparent molecular mass approximately equal to 600 kDa for the unreduced protein and an apparent molecular mass of 195 kDa after reduction by 2-mercaptoethanol [2].
• The sedimentation coefficient of CISP determined by ultracentrifugation on sucrose gradients was shifted from 9.7 S in the absence to 5.7 S in the presence of 2-mercaptoethanol [2].

Biological context of THBS2

• We report here the nucleotide sequence of a 386 bp RT-PCR fragment specific for CISP [3].
• This protein was recently purified in our laboratory, and its N-terminal amino-acid sequence revealed a significant similarity with thrombospondin-2 (TSP2) [3].
• Using glycosylation inhibitors, we could determine that CISP is synthesized as a 175-kDa core protein, is then matured into a 190-kDa high-mannose form and secreted as a 195-kDa mature protein [2].
• Recombinant mouse TSP2 inhibited BAE cell proliferation in response to LPA in a dose range similar to that of TSP1 [4].
• The overall distribution of TSP2 in the glomerulosa and fasciculata zones of the adrenal cortex, and its absence from the reticularis zone, argue in favor of a role in the protection of adrenocortical cells against apoptosis [5].

Anatomical context of THBS2

• The highest levels of TSP2 protein were found in the adrenal cortex, followed by the heart, spleen, brain, and kidney [6].
• Most endocrine tissues (adrenal cortex, testis, ovary, and placenta) appeared to express TSP2, as determined by Western blot analysis [6].
• Endothelial cell mitogenesis induced by LPA: inhibition by thrombospondin-1 and thrombospondin-2 [4].

Associations of THBS2 with chemical compounds

• Corticotropin-induced secreted protein (CISP) is a trimeric glycoprotein secreted by primary cultures of bovine adrenortical cells in response to adrenocorticotropic hormone (ACTH) [3].

Analytical, diagnostic and therapeutic context of THBS2

• We report here the purification of CISP to apparent homogeneity with an overall yield of 43% using a combination of heparin-agarose and Mono-Q chromatographies [7].
• The protein levels of TSP1 and CISP/TSP2 varied accordingly with their respective mRNA levels, as shown by immunoprecipitation and immunofluorescence experiments [3].
• The trimeric structure of CISP could be observed by rotary shadowing/electron microscopy, where CISP appeared to be composed of three equally electron-dense nodules and of a fourth nodule formed by the close association of three smaller fragments [2].

References