Disease relevance of nuc

• A secretion reporter system based on Staphylococcus aureus nuclease (nuc) was developed for use in mycobacteria [1].
• The staphylococcal nuclease (nuc) gene from Staphylococcus aureus has been cloned and expressed in Bacillus subtilis [2].

High impact information on nuc

• Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius [3].
• A simple and generally applicable strategy was used to obtain efficient homologous recombination between plasmids for purposes of mapping nuc- mutations, mapping second-site suppressors and constructing double mutant combinations from pairs of single mutations [4].
• A collection of 77 unique missense mutations distributed across the gene encoding staphylococcal nuclease (nuc) has been assembled [4].
• Four nuc- mutations which alter amino acid residues at positions outside of the active site region of the enzyme were submitted to a second round of mutagenesis, and characterization of several independent NUC+ isolates lead to the identification of three second-site suppressor mutations within the protein-coding sequence of the nuc gene [4].
• All investigated strains harbored the clfA, clfB, coa, spa, and nuc genes, but the presence of their products was not detected by the phenotypic methods [5].

Chemical compound and disease context of nuc

• A multiplex real-time polymerase chain reaction (RT-PCR) targeting the mecA and nuc genes was developed for the detection of methicillin resistance and identification of Staphylococcus aureus [6].
• OBJECTIVE: To evaluate polymerase chain reaction (PCR) analysis for detection of Staphylococcus aureus (nuc gene) in fresh and formalin-preserved milk [7].

Biological context of nuc

• Transposition of TnNuc into the L. lactis chromosome allows the generation of fusions in-frame with the nuc gene [8].
• The coefficients of correlation between log CFU and nuc gene copy numbers ranged from 0.979 to 0.998, thus enabling calculation of the number of CFU of S. aureus in cheese by performing RTQ-PCR [9].
• This in-situ detection system was used to identify secreted proteins by screening a pBPnuc1::H37Rv nuc gene fusion library in M. smegmatis [1].
• Isolates were characterized by PFGE, mecA and nuc PCR, transmission electron microscopy (TEM) and analysis of cell proteins (proteomics) [10].
• Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles [11].

Associations of nuc with chemical compounds

• A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance [12].
• Further characterization of isolates with discrepant results for oxacillin testing was done by PCR detection of the nuc and mecA genes [13].
• Multiplex PCR using touchdown annealing was used to detect the mecA, nuc, and 16S rRNA genes in bottles growing staphylococci [11].
• CONCLUSIONS: The PCR technique based on the nuc gene is able to detect S aureus in sheep milk yields results faster than does traditional culturing, is highly specific, and is able to detect S aureus in formalin-fixed milk samples [7].

Other interactions of nuc

• METHODS AND RESULTS: The strain Staph. aureus FRI 137 harbouring nuc, sec, seg, seh and sei genes was used in this study [14].

Analytical, diagnostic and therapeutic context of nuc

• Spectral profile data obtained were compared with the database provided with the instrument, and 74% of the isolates were identified as S. aureus, as confirmed by a nuc-based PCR test [15].
• The PCR product was detected by agarose gel electrophoresis or Southern blot analysis by using a 33-mer internal nuc gene hybridization probe [16].
• Results from the RT-PCR assay showed 5 isolates with lower efficiency fluorescence curves for the nuc gene PCR fragment [6].
• The high concordance between S. aureus and nuc PCR positive strains (99%) corroborates the specificity of the primers used and the suitability of nuc PCR for rapid identification of S. aureus in routine food analysis [17].
• The tests included seroinhibition by a monoclonal antibody (MAb) against S. aureus TNase, MAb-based detection of the TNase in a sandwich ELISA, and a polymerase chain reaction for amplification of the nuc gene encoding the S. aureus TNase [18].

References