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Gene Review

nuc  -  thermonuclease precursor

Staphylococcus aureus RF122

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Disease relevance of nuc


High impact information on nuc

  • Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius [3].
  • A simple and generally applicable strategy was used to obtain efficient homologous recombination between plasmids for purposes of mapping nuc- mutations, mapping second-site suppressors and constructing double mutant combinations from pairs of single mutations [4].
  • A collection of 77 unique missense mutations distributed across the gene encoding staphylococcal nuclease (nuc) has been assembled [4].
  • Four nuc- mutations which alter amino acid residues at positions outside of the active site region of the enzyme were submitted to a second round of mutagenesis, and characterization of several independent NUC+ isolates lead to the identification of three second-site suppressor mutations within the protein-coding sequence of the nuc gene [4].
  • All investigated strains harbored the clfA, clfB, coa, spa, and nuc genes, but the presence of their products was not detected by the phenotypic methods [5].

Chemical compound and disease context of nuc


Biological context of nuc

  • Transposition of TnNuc into the L. lactis chromosome allows the generation of fusions in-frame with the nuc gene [8].
  • The coefficients of correlation between log CFU and nuc gene copy numbers ranged from 0.979 to 0.998, thus enabling calculation of the number of CFU of S. aureus in cheese by performing RTQ-PCR [9].
  • This in-situ detection system was used to identify secreted proteins by screening a pBPnuc1::H37Rv nuc gene fusion library in M. smegmatis [1].
  • Isolates were characterized by PFGE, mecA and nuc PCR, transmission electron microscopy (TEM) and analysis of cell proteins (proteomics) [10].
  • Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles [11].

Associations of nuc with chemical compounds

  • A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance [12].
  • Further characterization of isolates with discrepant results for oxacillin testing was done by PCR detection of the nuc and mecA genes [13].
  • Multiplex PCR using touchdown annealing was used to detect the mecA, nuc, and 16S rRNA genes in bottles growing staphylococci [11].
  • CONCLUSIONS: The PCR technique based on the nuc gene is able to detect S aureus in sheep milk yields results faster than does traditional culturing, is highly specific, and is able to detect S aureus in formalin-fixed milk samples [7].

Other interactions of nuc

  • METHODS AND RESULTS: The strain Staph. aureus FRI 137 harbouring nuc, sec, seg, seh and sei genes was used in this study [14].

Analytical, diagnostic and therapeutic context of nuc

  • Spectral profile data obtained were compared with the database provided with the instrument, and 74% of the isolates were identified as S. aureus, as confirmed by a nuc-based PCR test [15].
  • The PCR product was detected by agarose gel electrophoresis or Southern blot analysis by using a 33-mer internal nuc gene hybridization probe [16].
  • Results from the RT-PCR assay showed 5 isolates with lower efficiency fluorescence curves for the nuc gene PCR fragment [6].
  • The high concordance between S. aureus and nuc PCR positive strains (99%) corroborates the specificity of the primers used and the suitability of nuc PCR for rapid identification of S. aureus in routine food analysis [17].
  • The tests included seroinhibition by a monoclonal antibody (MAb) against S. aureus TNase, MAb-based detection of the TNase in a sandwich ELISA, and a polymerase chain reaction for amplification of the nuc gene encoding the S. aureus TNase [18].


  1. Staphylococcus aureus nuclease is a useful secretion reporter for mycobacteria. Downing, K.J., McAdam, R.A., Mizrahi, V. Gene (1999) [Pubmed]
  2. Secretion of staphylococcal nuclease by Bacillus subtilis. Kovacevic, S., Veal, L.E., Hsiung, H.M., Miller, J.R. J. Bacteriol. (1985) [Pubmed]
  3. Nucleotide sequence of a nuc gene encoding the thermonuclease of Staphylococcus intermedius. Chesneau, O., el Solh, N. Nucleic Acids Res. (1992) [Pubmed]
  4. Genetic analysis of staphylococcal nuclease: identification of three intragenic "global" suppressors of nuclease-minus mutations. Shortle, D., Lin, B. Genetics (1985) [Pubmed]
  5. Characteristics of Staphylococcus aureus Strains Isolated in Poland in 1996 to 2004 That Were Deficient in Species-Specific Proteins. Luczak-Kadlubowska, A., Krzyszton-Russjan, J., Hryniewicz, W. J. Clin. Microbiol. (2006) [Pubmed]
  6. Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction. Costa, A.M., Kay, I., Palladino, S. Diagn. Microbiol. Infect. Dis. (2005) [Pubmed]
  7. Detection of Staphylococcus aureus in milk by use of polymerase chain reaction analysis. Khan, M.A., Kim, C.H., Kakoma, I., Morin, E., Hansen, R.D., Hurley, W.L., Tripathy, D.N., Baek, B.K. Am. J. Vet. Res. (1998) [Pubmed]
  8. The development of TnNuc and its use for the isolation of novel secretion signals in Lactococcus lactis. Ravn, P., Arnau, J., Madsen, S.M., Vrang, A., Israelsen, H. Gene (2000) [Pubmed]
  9. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time quantitative PCR and application of this technique for examination of cheese. Hein, I., Lehner, A., Rieck, P., Klein, K., Brandl, E., Wagner, M. Appl. Environ. Microbiol. (2001) [Pubmed]
  10. Identification and characterization of teicoplanin-intermediate Staphylococcus aureus blood culture isolates in NE Scotland. MacKenzie, F.M., Greig, P., Morrison, D., Edwards, G., Gould, I.M. J. Antimicrob. Chemother. (2002) [Pubmed]
  11. Direct detection of mecA, nuc and 16S rRNA genes in BacT/Alert blood culture bottles. Lem, P., Spiegelman, J., Toye, B., Ramotar, K. Diagn. Microbiol. Infect. Dis. (2001) [Pubmed]
  12. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures. Maes, N., Magdalena, J., Rottiers, S., De Gheldre, Y., Struelens, M.J. J. Clin. Microbiol. (2002) [Pubmed]
  13. Evaluation of Rapid ATB Staph for 5-hour antimicrobial susceptibility testing of Staphylococcus aureus. Groupement pour le Dépistage, L'Etude et la Prévention des Infections Hospitalières-Groep ter Opsporing, Studie en Preventie van Infecties in de Ziekenhuizen. Struelens, M.J., Nonhoff, C., van der Auwera, P., Mertens, R., Serruys, E. J. Clin. Microbiol. (1995) [Pubmed]
  14. PCR-based detection of enterotoxigenic Staphylococcus aureus in the early stages of raw milk cheese making. Ercolini, D., Blaiotta, G., Fusco, V., Coppola, S. J. Appl. Microbiol. (2004) [Pubmed]
  15. Identification of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Du, Z., Yang, R., Guo, Z., Song, Y., Wang, J. Anal. Chem. (2002) [Pubmed]
  16. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Brakstad, O.G., Aasbakk, K., Maeland, J.A. J. Clin. Microbiol. (1992) [Pubmed]
  17. Identification and typing of food-borne Staphylococcus aureus by PCR-based techniques. Pinto, B., Chenoll, E., Aznar, R. Syst. Appl. Microbiol. (2005) [Pubmed]
  18. Comparison of tests designed to identify Staphylococcus aureus thermostable nuclease. Brakstad, O.G., Maeland, J.A., Chesneau, O. APMIS (1995) [Pubmed]
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