Disease relevance of Ras64B

• We cloned a Drosophila melanogaster ras gene (Dmras64B) on the basis of its homology to the ras oncogen from Harvey murine sarcoma virus [1].
• Noonan syndrome and related disorders: dysregulated RAS-mitogen activated protein kinase signal transduction [2].

High impact information on Ras64B

• For example, components of the RAS signaling cascade were identified using a mutant eye phenotype in D. melanogaster as a readout [3].
• These results argue that photoreceptor differentiation is regulated by the RAS pathway through targeted proteolysis of the TTK repressor [4].
• Down-regulation requires the presence of Phyllopod (PHYL), which is induced by the RAS pathway, and Seven In Absentia (SINA) [4].
• R7 photoreceptor fate in the Drosophila eye induced by the activation of the Sevenless receptor tyrosine kinase and the RAS/MAP kinase signal transduction pathway [5].
• Two of these genes (termed Dras 1 and Dras 2) were sequenced [6].

Biological context of Ras64B

• In the case of Dras2, the nucleotide sequence fo the genomic clone was determined [6].
• The Drosophila Ras2 and Rop gene pair: a dual homology with a yeast Ras-like gene and a suppressor of its loss-of-function phenotype [7].
• The cellular signal transduction pathways by which C3G, a RAS family guanine nucleotide exchange factor, mediates v-crk transformation are not well understood [8].
• The transcription start sites of the ras2 gene and the flanking transcription unit were determined by external primer extension with T4 DNA polymerase and by RNAase-protection assay and were found to be only 94 nucleotides apart [9].
• We isolated and delimitated the Drosophila ras2 promoter region, determined its sequence and mapped the transcription units expressed in this region [9].

Anatomical context of Ras64B

• Rop and Ras2, members of the Sec1 and Ras families, are localized in the outer membranes of labyrinthine channels and vesicles of Drosophila nephrocyte, the garland cell [10].

Associations of Ras64B with chemical compounds

• In the yeast Saccharomyces cerevisiae, the RAS gene products serve as essential function by regulating cyclic adenosine monophosphate metabolism [11].

Other interactions of Ras64B

• We have also established that the Rop and Ras2 genes are located within the 63E-64A genomic domain at 64A10,11 [12].
• We describe the developmental effects of the expression of a normal and mutated Drosophila ras gene ras2 in transgenic Drosophila [13].

Analytical, diagnostic and therapeutic context of Ras64B

• A Gly14----Val14 mutant (Gly14 in ras2 is equivalent to Gly12 in mammalian ras proteins) was constructed in ras2 by site-directed mutagenesis [13].

References