Disease relevance of ESR1

• Transmission of F4+ E. coli was quantified in four heterogeneous groups of F4R+ and F4R- piglets [1].

High impact information on ESR1

• Quantitative examinations revealed that not only the number of lysosomes in the proximity of the nucleus but also their immunostaining for estradiol receptor alpha increased significantly after the hormone pulse [2].
• The low-sterically hindered immunoreagents--recognizing different epitopes within the hormone binding domain--allowed for an efficient immunolabeling of estradiol receptor alpha, detecting it both in the cytoplasm and the nucleus of nonstimulated epithelium cells [2].
• Retrieval of estradiol receptor in paraffin sections of resting porcine uteri by microwave treatment. Immunostaining patterns obtained with different primary antibodies [3].
• Completion of the amino acid sequence of the C-terminal half of the porcine estradiol receptor by Edman degradation: reconfirmation of the absence of O-linked sugars and phosphates [4].
• The C-terminal half of the porcine estradiol receptor contains no post-translational modification: determination of the primary structure [5].

Biological context of ESR1

• ESR1 alleles displayed different patterns of linkage disequilibrium with the causal mutation in each of the parental populations and the PvuII polymorphism was clearly not the causal mutation [6].
• The side chains responsible for the dimerization of the estradiol receptor by ionic bonds are lost in a 17 kDa fragment extending downstream from aa 303 [7].
• Impact of the ESR gene on litter size and production traits in Czech Large White pigs [8].
• Oral administration of F4 fimbriae of Escherichia coli induces intestinal mucosal immune responses in F4 receptor-positive (F4R(+)) pigs, but not in F4R(-) pigs [9].
• A goat antiserum raised against the preparation contains immunoglobulins G precipitating estradiol-receptor complexes, and antibodies releasing the steroid from its binding site [10].

Anatomical context of ESR1

• This was expected in F4R(+) pigs, as in these animals oral F4 induces F4-specific antibody-secreting cells in the spleen, suggesting a priming of the systemic immune system [9].
• Moreover points to AhR dependent but not ER-dependent mechanisms in TCDD action in luteal cells [11].
• The exhaustive extraction of microsomal estradiol receptor by Surfynol/dithiothreitol/trypsin in low ionic strength buffer was employed for distribution studies on non-stimulated porcine endometrium [12].
• Two electrophoretically different forms of estradiol receptor can be extracted from crude porcine endometrium microsomes with low ionic strength buffers [13].
• The steroid-binding core of estradiol receptor was purified from pig uterus cytosol by a protocol consisting of (1) adsorption to heparin-sepharose, (2) enzymatic release of the receptor core, (3) DEAE-chromatography, (4) Sephadex G-150 filtration and (5) chromatography on heparin-sepharose [10].

Associations of ESR1 with chemical compounds

• The C-terminal part of ligand filled porcine estradiol receptor extending from H267 to I595 was isolated by adsorption to the monoclonal antibody 13H2, subjected to cleavage by CNBr, o-iodosobenzoic acid and endopeptidase Lys-C as well as other proteases, both in the native and the denatured state [5].
• The isolated nuclei were analysed for their content of estradiol and of estradiol receptor [14].
• OBJECTIVE: To examine whether the action of triiodothyronine on aromatase activity in porcine follicular cells is related to the modulation of estradiol receptor [15].
• We have tested the functional role of the estradiol receptor in these biphasic responses by utilizing the highly selective estrogen-receptor antagonist, LY156758, and the synthetic estrogen agonist, moxestrol [16].

Other interactions of ESR1

• While significantly high levels of nuclear estradiol receptor were found at the inter-embryonic site on day 9 of pregnancy, the cytosolic and nuclear progesterone receptor concentrations were greater at the embryonic site on the same day [17].

Analytical, diagnostic and therapeutic context of ESR1

• Indeed, the presence of conformational epitopes was shown by ELISA and the ability to bind the F4R was observed by inhibiting the adhesion of F4+ ETEC to F4R+ villi with increasing concentrations of native-like refolded rFaeG subunits [18].
• Use of meta-analysis to combine candidate gene association studies: application to study the relationship between the ESR PvuII polymorphism and sow litter size [19].

References