Disease relevance of LIM2

• In the present study, we show that FOXO1 interacts with four and a half LIM 2 (FHL2) in prostate cancer cells [1].

Psychiatry related information on LIM2

• When somatic cell hybrid DNAs were restricted with Eco R1 and Southern blots hybridized with the bovine MP19 cDNA, the characteristic human restriction pattern was observed only when human chromosome 19 was present in the hybrid panel [2].

High impact information on LIM2

• A missense mutation in the LIM2 gene is associated with autosomal recessive presenile cataract in an inbred Iraqi Jewish family [3].
• Sequencing of LIM2, one of two candidate genes in this region, revealed a homozygous T-->G change resulting in a phenylalanine-to-valine substitution at position 105 of the protein [3].
• Seven cysteinyl residues and a single histidyl residue in the LIM2 sequence, CX2CX17HX2CX2CX2CX17CX2C, comprise the conserved residues in the LIM consensus that are potential Zn(II) ligands [4].
• Two Zn(II) binding sites exhibiting tetrathiolate (S4) and S3N1 Zn(II) coordination are displayed by LIM2 (Kosa, J. L., Michelsen, J. W., Louis, H. A., Olsen, J. I., Davis, D. R., Beckerle, M. C., and Winge, D. R. (1994) Biochemistry 33, 468-477) [4].
• Site-directed mutagenesis was employed to generate three mutant LIM2 proteins with conversions of the second conserved cysteine to histidine (C2H), the fifth conserved cysteine to histidine (C5H), and the last conserved cysteine to aspartate (C8D) [4].

Biological context of LIM2

• 4. The position of the LIM2 gene appears to be within 40 kb of the electron transport flavoprotein gene (ETFB) as a cosmid containing sequences from both genes has been identified [5].
• Assignment of the human lens fiber cell MP19 gene (LIM2) to chromosome 19q13.4, and adjacent to ETFB [5].
• Perturbation of LIM3 or 4 inhibited adhesion while mutation of LIM2 or 4 decreased cell motility [6].
• Since the LIM2 gene promoter does not contain a classic TATA box, the Hsu element may serve as the site for binding the RNA polymerase complex [7].
• Analyses of ESTs also reveal examples of alternative transcripts, including a major alternative splice form for the lens specific membrane protein MP19 [8].

Anatomical context of LIM2

• This cDNA was used as a probe to analyze a panel of Southern blots of human-Chinese hamster somatic cell hybrid DNAs to assign the gene coding for MP19 to its human chromosome [2].
• RESULTS: MP20 and galectin-3 co-localized in selected areas of the lens fiber cell plasma membrane [9].

Associations of LIM2 with chemical compounds

• Binding between MP20 and galectin-3 was disrupted by lactose suggesting the lectin site was involved in the interaction [9].

Other interactions of LIM2

• The discovery of an abundant, novel transcript, lengsin, and a major novel splice form of MP19 reflect the utility of unamplified libraries constructed from dissected tissue [8].

Analytical, diagnostic and therapeutic context of LIM2

• The human lens fiber-cell intrinsic membrane protein MP19 gene: isolation and sequence analysis [10].

References