High impact information on DYNC1H1

• Cytoplasmic dynein is a minus end-directed motor for membranous organelles [1].
• Addition of purified cytoplasmic dynein to the inactive cytosol restored minus end-directed organelle motility, although purified cytoplasmic dynein by itself did not support organelle movement [1].
• The role of cytoplasmic dynein in microtubule-based organelle transport was examined using a reconstituted assay developed from chick embryo fibroblasts [1].
• Cytoplasmic dynein is a microtubule minus-end-directed motor that is thought to power the transport of vesicles from the TGN to the apical cortex in polarized epithelial cells [2].
• Motility driven by the microtubule motors, kinesin and cytoplasmic dynein, is inhibited by MAP2 (López, L. A., and Sheetz, M. P. (1993) Cell Motil. Cytoskeleton 24, 1-16) [3].

Biological context of DYNC1H1

• We suggest that there are two distinguishable binding sites for kinesin and cytoplasmic dynein on these organelles in the presence of ATP and a shared site in the absence of ATP [4].
• Cytoplasmic dynein is a multi-subunit complex involved in retrograde organelle transport and some aspects of mitosis [5].
• Cytoplasmic dynein, a large minus-end-directed microtubule motor, performs multiple functions during the cell cycle [6].
• It was also constantly transported from kinetochores to spindle poles by cytoplasmic dynein [7].

Anatomical context of DYNC1H1

• Inactivation of cytoplasmic dynein in the high-speed cytosol by vanadate-mediated UV photocleavage inhibited minus end-directed organelle motility by over 90% [1].
• Kinesin and cytoplasmic dynein binding to brain microsomes [4].
• Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles [8].

References