Disease relevance of Dync1h1

• Effect of various doses (125, 250, 500, and 1000 mg/kg, p.o.) of DHC-1 was studied on gastric secretion and gastric ulcers in pylorus-ligation and on ethanol-induced gastric mucosal injury in rats [1].
• The protective effect of DHC-1 in isoproterenol-induced myocardial infarction and cisplatin-induced renal damage were studied [2].

High impact information on Dync1h1

• The N-terminal third of the two polypeptides shows no clear relationship, suggesting that this region of MAP 1C is responsible for its association with retrograde organelles and other functions [3].
• Thus, in contrast with cilia and flagella, which contain numerous forms of dynein, our results are consistent with the existence of only a single cytoplasmic dynein heavy chain gene, which appears to produce only a single transcript [3].
• HMW-2 was selectively released from testis microtubules by ATP and co-purified by sucrose density gradient centrifugation with MAP-1C, a neuronal cytoplasmic dynein [4].
• We found that MAP 1 can be resolved by SDS PAGE into three electrophoretic bands, which we have designated MAP 1A, MAP 1B, and MAP 1C in order of increasing electrophoretic mobility [5].
• Another interesting feature of this 1,25-D3 induced-cell death program is the down-regulated expression of transcripts for the microtubule motor dynein heavy chain/MAP 1C and of the calcium-binding S100beta protein [6].

Biological context of Dync1h1

• Distinct but overlapping sites within the cytoplasmic dynein heavy chain for dimerization and for intermediate chain and light intermediate chain binding [7].
• A time course analysis during ciliated cell differentiation of RTE cells in culture demonstrated that the expression of axonemal dynein heavy chains correlated with the development of ciliated cells, while cytoplasmic dynein heavy chain expression remained constant [8].
• The trunk for all the cytoplasmic dynein heavy chain homologues diverged directly from the root of the phylogenetic tree, suggesting that the first dynein gene duplication defined two distinct functions as respective subfamilies [9].
• We have sequenced 550 plasmid clones which turned out to include 13 kinds of new dynein-like sequences (DLP1-8, 9A/B, 10-12) and cytoplasmic dynein heavy chain [9].
• The library was examined for inhibition of cytoplasmic dynein heavy chain and cell growth [10].

Anatomical context of Dync1h1

• A novel cytoplasmic dynein heavy chain: expression of DHC1b in mammalian ciliated epithelial cells [11].

Associations of Dync1h1 with chemical compounds

• Synthesis and biological evaluation of purealin and analogues as cytoplasmic dynein heavy chain inhibitors [10].
• A significant reduction in the serum markers of heart and kidney damage and the extent of lipid peroxidation with a concomitant increase in the enzymatic (SOD and CAT) and non-enzymatic antioxidants (reduced glutathione) were observed in DHC-1 pretreated animals compared with the isoproterenol or cisplatin alone treated animals [2].

Other interactions of Dync1h1

• A microtubule associated protein from brain tissue (MAP 1C), has been found to possess many properties in common with ciliary and flagellar dyneins (Paschal et al.:J. Cell Biol. 105:1273-1282, 1987) [12].

Analytical, diagnostic and therapeutic context of Dync1h1

• Molecular cloning of the retrograde transport motor cytoplasmic dynein (MAP 1C) [3].
• In this report, we demonstrate the localization of cytoplasmic dynein and manchette development by a double immunofluorescence technique using anti-bovine brain MAP 1C and anti-tubulin [13].

References