Disease relevance of MFI2

• Transfected mouse melanoma lines that express various levels of human melanoma-associated antigen p97 [1].
• These data demonstrate that in IMR-32 human neuroblastoma cells, zinc and cadmium can use independent mechanisms for activation of the mMT-I promoter and cadmium-mediated MRE activation is independent of MTF-1 and zinc [2].
• Recent studies using a knockout mouse and post-transcriptional gene silencing have demonstrated that MTf is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis [3].

High impact information on MFI2

• Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3 [4].
• Human melanoma-associated antigen p97 is structurally and functionally related to transferrin [5].
• The gene for the human melanoma-associated antigen p97 has been introduced by cDNA transfection into cells from clone M2 of the K1735 mouse melanoma, which metastasizes to the lung when injected iv into syngeneic C3H/HeN mice [1].
• These results suggest that regulation of metallothionein genes by metals is mediated by MTF-1 interacting with metal response elements and that zinc functions to release MTF-1 from an inhibitor [6].
• Our results indicate that p53 along with two other identified proteins, MTF-1 and La, post-transcriptionally regulate the synthesis of the S25 protein by controlling the nuclear export of the stress-induced S25 mRNA [7].

Biological context of MFI2

• Here we show that two other zinc finger factors, MTF-1 and Krox-20, can also bind to CpG methylated sites [8].
• Blood flow was measured by venous occlusion plethysmography in six hypertensive (mean arterial blood pressure (MAP) 113 +/- 4 mmHg) and six matched normotensive subjects (MAP 97 +/- 3 mmHg) [9].
• Differential sensitivity of zinc finger transcription factors MTF-1, Sp1 and Krox-20 to CpG methylation of their binding sites [8].

Anatomical context of MFI2

• These results indicate that MTF-1 is particularly important for proper hepatocyte proliferation [10].
• A bispecific monoclonal antibody (bsmAb) has been developed against the human melanoma-associated antigen p97 and an octahedral gallium chelate (Ga-HBED) using the hybrid hybridoma technology [11].
• Seven different human melanoma cell lines have been tested for the presence of melanoma associated antigen p97 [12].

Associations of MFI2 with chemical compounds

• Development of a bispecific monoclonal antibody against a gallium-67 chelate and the human melanoma-associated antigen p97 for potential use in pretargeted immunoscintigraphy [11].

Analytical, diagnostic and therapeutic context of MFI2

• Localization of melanoma-associated antigen p97 in cultured human melanoma, as visualized by light and electron microscopy [13].

References