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Gene Review

NFYC  -  nuclear transcription factor Y, gamma

Homo sapiens

Synonyms: CAAT box DNA-binding protein subunit C, CBF-C, CBFC, H1TF2A, HAP5, ...
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Disease relevance of NFYC

  • The acrylic and HSM PMMA groups had the lowest incidence of relapses [1].
  • Polymorphism in anhydrous picotamide, i.e., nucleation of crystal forms A, mp 135.5 +/- 0.4 degrees C, and B, mp 152.9 +/- 0.3 degrees C after dehydration of PICOW, was detected by DSC and HSM [2].
  • CONCLUSION: No clinical advantage was found when the HSM IOL was compared with the regular PMMA IOL in 43 patients with uveitis [3].

High impact information on NFYC

  • We describe here the basis for this strict requirement by demonstrating that both ATF6alpha and ATF6beta physically interact with NF-Y trimer via direct binding to the NF-YC subunit [4].
  • We then performed a mutational analysis of CBF-C to define functional interactions with the other two CBF subunits and with DNA using several in vitro assays and an in vivo yeast two-hybrid system [5].
  • Analysis of the first class of mutants revealed that a major part of the conserved segment was essential for interactions with CBF-C to form a heterodimeric CBF-A/CBF-C complex [6].
  • The cloning of H1TF2A, the large subunit of this factor, reveals it to be a glutamine-rich protein with extremely limited similarity to previously cloned CCAAT-binding proteins [7].
  • The structure together with the modeling of the nonspecific interaction of NF-YC/NF-YB with DNA and the full NF-Y/CCAAT box complex highlight important structural features that account for different and possibly similar biological functions of the transcriptional regulators NF-Y and NC2 [8].

Biological context of NFYC

  • A point mutation in the NFYC gene generates an antigenic peptide recognized by autologous cytolytic T lymphocytes on a human squamous cell lung carcinoma [9].
  • The segment of CBF-A necessary for DNA binding showed sequence homology to a segment of CBF-C [6].
  • The isolated human CBF-C cDNA is 1977 nt long and consists of the full-length 3'-untranslated region (781 nt) with a poly (A) tail at the 3' end 185 nucleotides of 5'-untranslated region and the open reading frame (1011 nt), encoding a 337-aa protein with 91.7% homology with the translated region of rat CBF-C cDNA [10].
  • At least eight genes with homologies to CBF-C are present in the Arabidopsis genome; one of them exhibits striking similarities to the gene for the human global transcriptional repressor Drap1 [11].
  • Speech intelligibility in quiet and CCITT noise from the side (+/-90 degrees ) was assessed using the German HSM sentence test and was significantly better when using bilateral CI in comparison with either unilateral CI, mainly due to a head shadow effect [12].

Anatomical context of NFYC


Associations of NFYC with chemical compounds

  • CONCLUSIONS: No significant difference was observed in inflammation between eyes having HSM PMMA IOL implantation or those having soft hydrophobic acrylic IOL implantation through the same-size incision [15].
  • HSM studies indicated that the Gelucire crystallised into two main spherulitic conformations; paracetamol appeared to act as a nucleation site for the lower melting fractions while caffeine particles changed into a needle-shaped morphology on cooling the system from the liquid state [16].

Physical interactions of NFYC

  • The Bdbd polypeptide interacts with cellular CBF-A/CBF-C and binds to promoters containing CBF-binding sites [17].

Other interactions of NFYC

  • Two subunits, NF-YB and NF-YC, contain a histone-like motif [13].
  • Chromosomal assignment and tissue expression of CBF-C/NFY-C, the third subunit of the mammalian CCAAT-binding factor [18].
  • We expressed and purified the yeast homology domain of NF-YC in bacteria and performed EMSA together with the corresponding conserved domains of NF-YA and NF-YB, obtaining a CCAAT-binding mini-NF-Y [13].
  • In particular, it emphasizes the role of the newly described alphaC helix of NF-YC, which is both important for NF-Y trimerization and a target for regulatory proteins, such as MYC and p53 [8].
  • The CCAAT-activator NF-Y and DeltaNp63alpha are associated in vivo and a conserved alpha-helix of the NF-YC histone fold is required. p63 AEC mutants, but not an EEC mutant, are incapable to bind NF-Y [19].

Analytical, diagnostic and therapeutic context of NFYC

  • Sedimentation velocity centrifugation experiments confirm that two pools of NF-YB, and most likely NF-YC, exist: one associated with NF-YA and binding to the CCAAT box; another involved in high molecular weight complexes [20].
  • This indicates that hydrophobic acrylic and HSM PMMA materials induce the same degree of BAB breakdown after phacoemulsification in eyes of diabetic patients [15].
  • RESULTS: Posterior capsule opacification developed in 47 cases (15.6%): 21.7% in the PMMA lens group after planned extracapsular cataract extraction (ECCE), 17.4% in the HSM PMMA lens group after planned ECCE, and 7.7% in the plate-haptic silicone lens group after phacoemulsification [21].
  • Dispersions containing up to 30% w/w drug were prepared and studied using differential scanning calorimetry (DSC), hot stage differential interference contrast microscopy (HSM), dissolution studies and erosion, water uptake (WU) and diameter change measurements [16].
  • The HSM sentence test as a tool for evaluating the speech understanding in noise of cochlear implant users [22].


  1. Comparative performance of intraocular lenses in eyes with cataract and uveitis. Alió, J.L., Chipont, E., BenEzra, D., Fakhry, M.A. Journal of cataract and refractive surgery. (2002) [Pubmed]
  2. Physical characterization of picotamide monohydrate and anhydrous picotamide. Bettinetti, G., Mura, P., Sorrenti, M., Faucci, M.T., Negri, A. Journal of pharmaceutical sciences. (1999) [Pubmed]
  3. A retrospective analysis of heparin-surface-modified intraocular lenses versus regular polymethylmethacrylate intraocular lenses in patients with uveitis. Lardenoye, C.W., van der Lelij, A., Berendschot, T.T., Rothova, A. Documenta ophthalmologica. Advances in ophthalmology. (1996) [Pubmed]
  4. Endoplasmic reticulum stress-induced formation of transcription factor complex ERSF including NF-Y (CBF) and activating transcription factors 6alpha and 6beta that activates the mammalian unfolded protein response. Yoshida, H., Okada, T., Haze, K., Yanagi, H., Yura, T., Negishi, M., Mori, K. Mol. Cell. Biol. (2001) [Pubmed]
  5. Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts simultaneously with both the CBF-A and CBF-C subunits to form a heterotrimeric CBF molecule. Kim, I.S., Sinha, S., de Crombrugghe, B., Maity, S.N. Mol. Cell. Biol. (1996) [Pubmed]
  6. Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex. Sinha, S., Kim, I.S., Sohn, K.Y., de Crombrugghe, B., Maity, S.N. Mol. Cell. Biol. (1996) [Pubmed]
  7. H1TF2A, the large subunit of a heterodimeric, glutamine-rich CCAAT-binding transcription factor involved in histone H1 cell cycle regulation. Martinelli, R., Heintz, N. Mol. Cell. Biol. (1994) [Pubmed]
  8. The NF-YB/NF-YC structure gives insight into DNA binding and transcription regulation by CCAAT factor NF-Y. Romier, C., Cocchiarella, F., Mantovani, R., Moras, D. J. Biol. Chem. (2003) [Pubmed]
  9. A point mutation in the NFYC gene generates an antigenic peptide recognized by autologous cytolytic T lymphocytes on a human squamous cell lung carcinoma. Takenoyama, M., Baurain, J.F., Yasuda, M., So, T., Sugaya, M., Hanagiri, T., Sugio, K., Yasumoto, K., Boon, T., Coulie, P.G. Int. J. Cancer (2006) [Pubmed]
  10. Isolation and sequence analysis of the cDNA encoding subunit C of human CCAAT-binding transcription factor. Dmitrenko, V., Garifulin, O., Kavsan, V. Gene (1997) [Pubmed]
  11. The assembly of the CAAT-box binding complex at a photosynthesis gene promoter is regulated by light, cytokinin, and the stage of the plastids. Kusnetsov, V., Landsberger, M., Meurer, J., Oelmüller, R. J. Biol. Chem. (1999) [Pubmed]
  12. Minimum audible angle, just noticeable interaural differences and speech intelligibility with bilateral cochlear implants using clinical speech processors. Senn, P., Kompis, M., Vischer, M., Haeusler, R. Audiol. Neurootol. (2005) [Pubmed]
  13. Cloning and expression of human NF-YC. Bellorini, M., Zemzoumi, K., Farina, A., Berthelsen, J., Piaggio, G., Mantovani, R. Gene (1997) [Pubmed]
  14. Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y. Taira, T., Sawai, M., Ikeda, M., Tamai, K., Iguchi-Ariga, S.M., Ariga, H. J. Biol. Chem. (1999) [Pubmed]
  15. Aqueous flare induced by heparin-surface-modified poly(methyl methacrylate) and acrylic lenses implanted through the same-size incision in patients with diabetes. Gatinel, D., Lebrun, T., Le Toumelin, P., Chaine, G. Journal of cataract and refractive surgery. (2001) [Pubmed]
  16. The influence of drug incorporation on the structure and release properties of solid dispersions in lipid matrices. Khan, N., Craig, D.Q. Journal of controlled release : official journal of the Controlled Release Society. (2003) [Pubmed]
  17. Inhibition of CBF/NF-Y mediated transcription activation arrests cells at G2/M phase and suppresses expression of genes activated at G2/M phase of the cell cycle. Hu, Q., Lu, J.F., Luo, R., Sen, S., Maity, S.N. Nucleic Acids Res. (2006) [Pubmed]
  18. Chromosomal assignment and tissue expression of CBF-C/NFY-C, the third subunit of the mammalian CCAAT-binding factor. Sinha, S., Maity, S.N., Seldin, M.F., de Crombrugghe, B. Genomics (1996) [Pubmed]
  19. Mechanisms of transcriptional repression of cell-cycle G2/M promoters by p63. Testoni, B., Mantovani, R. Nucleic Acids Res. (2006) [Pubmed]
  20. CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues. Bellorini, M., Lee, D.K., Dantonel, J.C., Zemzoumi, K., Roeder, R.G., Tora, L., Mantovani, R. Nucleic Acids Res. (1997) [Pubmed]
  21. Effect of intraocular lens and anterior capsule opening type on posterior capsule opacification. Birinci, H., Kuruoğlu, S., Oge, I., Oge, F., Acar, E. Journal of cataract and refractive surgery. (1999) [Pubmed]
  22. The HSM sentence test as a tool for evaluating the speech understanding in noise of cochlear implant users. Hochmair-Desoyer, I., Schulz, E., Moser, L., Schmidt, M. The American journal of otology. (1997) [Pubmed]
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