High impact information on ODF2

• Moreover, this 96-kD protein, which we name Cenexin (derived from the Latin, senex for "old man," and Cenexin for centriole) defines very precisely the mature centriole of a pair and is acquired by the immature centriole at the G2/M transition in prophase [1].
• Exogenously expressed and endogenous Odf2 were shown to be concentrated at the centrosomes in a microtubule-independent manner in various types of cells at both light and electron microscopic levels [2].
• Odf2 exhibited a cell cycle-dependent pattern of localization and was preferentially associated with the mother centrioles in G0/G1-phase [2].
• Interactional cloning of the 84-kDa major outer dense fiber protein Odf84. Leucine zippers mediate associations of Odf84 and Odf27 [3].
• Outer dense fiber 2 (Odf2) is highly expressed in the testis where it encodes a major component of the outer dense fibers of the sperm flagellum [4].

Biological context of ODF2

• From a human testis cDNA library, we isolated two slightly different cDNAs encoding ODF2 proteins of approximately 70 kDa [5].
• Human outer dense fiber gene, ODF2, localizes to chromosome 9q34 [6].
• The two protein spots identified by 2D WB namely TSA1-pI=5.821, M(W)=77.050 and TSA3-pI=6.173, M(W)=75.519 showed sequence homology to Cenexin/odf2 indicating that two are isoforms of the same protein [7].
• Furthermore, no homozygous embryos were found at the blastocyst stage of embryonic development, implying a critical pre-implantation role for Odf2 [4].

Anatomical context of ODF2

• ODF2 proteins were detected in the sperm tail [5].
• Transcription of ODF2 is restricted to testis tissue and more specifically to round spermatids as was demonstrated by a non-radioactive in-situ hybridization [5].
• We have generated Odf2 knockout mice using an Odf2 gene trapped embryonic stem cell (ESC) line [4].
• Odf2 transcripts could be demonstrated in testicular RNA and are restricted to germ cells [8].

Other interactions of ODF2

• Specifically, more and more novel proteins associated with centrosome such as centrin, centriolin and cenexin are located in the centriole, a core component of cenrtrosome [9].

Analytical, diagnostic and therapeutic context of ODF2

• We find that the novel gene, 111-450, encodes a product that localizes to ODF as determined by fluorescence microscopy and immunoelectron microscopy and that the gene 111-450 product is identical to the major ODF protein, Odf84 [3].

References