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Gene Review

CETN1  -  centrin, EF-hand protein, 1

Homo sapiens

Synonyms: CEN1, CETN, Caltractin isoform 2, Centrin-1
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Disease relevance of CETN1

  • To determine the structure of human centrin-2 and to develop an understanding of molecular interactions between centrin and xeroderma pigmentosum group C protein, we characterized the crystal structure of calcium-loaded full-length centrin-2 complexed with a xeroderma pigmentosum group C peptide [1].
  • The centrin null mutant defective in amastigote growth could be useful as a vaccine candidate against leishmaniasis [2].
  • The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method [3].
  • To continue testing, the SET proteins associated with eukaryotic motility (tektin, centrin and calmodulin) were sought in Spirochaeta halophila [4].
  • Although neither centrin nor calmodulin were detected, a 30 kDa tektin-like protein apparently is present in these spirochetes [4].

High impact information on CETN1

  • Centrin is a ubiquitous protein component of centrosomes and mitotic spindle poles. cDNA clones encoding centrin have been identified from vertebrate sources, and their sequences demonstrate that centrin is a highly conserved member of the EF-hand superfamily of calcium-binding proteins [5].
  • Centriole assembly begins with the formation of small centrin aggregates that appear during the S phase [6].
  • We have generated several stable cell lines expressing GFP-labeled centrin [7].
  • The evolutionary conservation of centrin-related sequences and immunologic epitopes to microtubule organizing centers of divergent phylogeny suggests that a functional attribute(s) may have been conserved as well [8].
  • We conclude that the 165,000-Mr protein is immunologically related to centrin, and that it is a component of a novel centrosome-associated cytoskeletal filament system [8].

Biological context of CETN1


Anatomical context of CETN1


Associations of CETN1 with chemical compounds


Physical interactions of CETN1

  • The present results suggest that almost all of the repeats of the Sfi1 protein may independently bind centrin molecules [16].
  • The interface between centrin-2 and each peptide is predominantly nonpolar, and key hydrophobic residues of XPC have been identified that lead us to propose a novel binding motif for centrin [1].
  • Recent findings demonstrate that Ca2+-activated centrin forms a complex with the visual G-protein transducin in photoreceptor cells [17].

Other interactions of CETN1

  • It also shows a differential localisation of centrin proteins in mature centriole/basal bodies, suggesting different functions for centrins 1/2 and centrin 3 [9].
  • This is also supported by functional analyses showing that centrin 1 and/or centrin 2 are involved in ciliary beating [9].
  • On the basis of this hypothesis and previous studies on centrin self-assembly, we propose a working model for the role of centrin-Sfi1 interactions in the dynamic structure of centrosome-associated contractile fibers [16].
  • This analysis detected the majority of known centriole proteins, including centrin, epsilon tubulin, and the cartwheel protein BLD10p [18].
  • Immunochemical evidence indicates that at least several of the proteins found in mammalian centrosomes, gamma-tubulin, centrin, pericentrin, and polypeptides recognized by the monoclonal antibodies MPM-2, 6C6, and C9 also recognize putative lower land plant MTOCs, indicating shared mechanisms of nucleation/organization in plants and animals [19].

Analytical, diagnostic and therapeutic context of CETN1


  1. The structure of the human centrin 2-xeroderma pigmentosum group C protein complex. Thompson, J.R., Ryan, Z.C., Salisbury, J.L., Kumar, R. J. Biol. Chem. (2006) [Pubmed]
  2. Centrin gene disruption impairs stage-specific basal body duplication and cell cycle progression in Leishmania. Selvapandiyan, A., Debrabant, A., Duncan, R., Muller, J., Salotra, P., Sreenivas, G., Salisbury, J.L., Nakhasi, H.L. J. Biol. Chem. (2004) [Pubmed]
  3. Preliminary x-ray crystallographic analysis of centrin from ciliate Euplotes octocarinatus. He, X.J., Li, L., Hou, H., Zhao, Y., Liang, A., Rao, Z. Protein Pept. Lett. (2005) [Pubmed]
  4. Search for eukaryotic motility proteins in spirochetes: immunological detection of a tektin-like protein in Spirochaeta halophila. Barth, A.L., Stricker, J.A., Margulis, L. BioSystems (1991) [Pubmed]
  5. Centrin, centrosomes, and mitotic spindle poles. Salisbury, J.L. Curr. Opin. Cell Biol. (1995) [Pubmed]
  6. The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation. La Terra, S., English, C.N., Hergert, P., McEwen, B.F., Sluder, G., Khodjakov, A. J. Cell Biol. (2005) [Pubmed]
  7. The respective contributions of the mother and daughter centrioles to centrosome activity and behavior in vertebrate cells. Piel, M., Meyer, P., Khodjakov, A., Rieder, C.L., Bornens, M. J. Cell Biol. (2000) [Pubmed]
  8. Identification and localization of a novel, cytoskeletal, centrosome-associated protein in PtK2 cells. Baron, A.T., Salisbury, J.L. J. Cell Biol. (1988) [Pubmed]
  9. Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro. Laoukili, J., Perret, E., Middendorp, S., Houcine, O., Guennou, C., Marano, F., Bornens, M., Tournier, F. J. Cell. Sci. (2000) [Pubmed]
  10. Phosphorylation of centrin during the cell cycle and its role in centriole separation preceding centrosome duplication. Lutz, W., Lingle, W.L., McCormick, D., Greenwood, T.M., Salisbury, J.L. J. Biol. Chem. (2001) [Pubmed]
  11. Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles. Errabolu, R., Sanders, M.A., Salisbury, J.L. J. Cell. Sci. (1994) [Pubmed]
  12. Estrogen mediates Aurora-A overexpression, centrosome amplification, chromosomal instability, and breast cancer in female ACI rats. Li, J.J., Weroha, S.J., Lingle, W.L., Papa, D., Salisbury, J.L., Li, S.A. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  13. Expression of a mutant form of Leishmania donovani centrin reduces the growth of the parasite. Selvapandiyan, A., Duncan, R., Debrabant, A., Bertholet, S., Sreenivas, G., Negi, N.S., Salotra, P., Nakhasi, H.L. J. Biol. Chem. (2001) [Pubmed]
  14. Phosphorylation of algal centrin is rapidly responsive to changes in the external milieu. Martindale, V.E., Salisbury, J.L. J. Cell. Sci. (1990) [Pubmed]
  15. Obstacles and opportunities for the multidisciplinary wound care team. A report for the clinical symposium on wound management. Baranoski, S., Salzberg, C.A., Staley, M.J., Thomas, D.R., Ayello, E.A. Advances in wound care : the journal for prevention and healing. (1998) [Pubmed]
  16. Binding of human centrin 2 to the centrosomal protein hSfi1. Martinez-Sanz, J., Yang, A., Blouquit, Y., Duchambon, P., Assairi, L., Craescu, C.T. FEBS J. (2006) [Pubmed]
  17. Centrins, a novel group of Ca2+-binding proteins in vertebrate photoreceptor cells. Wolfrum, U., Giessl, A., Pulvermüller, A. Adv. Exp. Med. Biol. (2002) [Pubmed]
  18. Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes. Keller, L.C., Romijn, E.P., Zamora, I., Yates, J.R., Marshall, W.F. Curr. Biol. (2005) [Pubmed]
  19. Microtubule-organizing centers and nucleating sites in land plants. Vaughn, K.C., Harper, J.D. Int. Rev. Cytol. (1998) [Pubmed]
  20. Most of centrin in animal cells is not centrosome-associated and centrosomal centrin is confined to the distal lumen of centrioles. Paoletti, A., Moudjou, M., Paintrand, M., Salisbury, J.L., Bornens, M. J. Cell. Sci. (1996) [Pubmed]
  21. Expression of centrin isoforms in the mammalian retina. Wolfrum, U., Salisbury, J.L. Exp. Cell Res. (1998) [Pubmed]
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