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Gene Review

ODF1  -  outer dense fiber of sperm tails 1

Homo sapiens

Synonyms: CT133, HSPB10, ODF, ODF2, ODF27, ...
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Disease relevance of ODF1


High impact information on ODF1

  • The TNF-family molecule RANK-L (RANK-L, TRANCE, ODF) and its receptor RANK are key regulators of bone remodeling, and they are essential for the development and activation of osteoclasts [2].
  • Outer dense fiber 2 (Odf2) was initially identified as a major component of sperm tail cytoskeleton and later was suggested to be a widespread component of centrosomal scaffold that preferentially associates with the appendages of the mother centrioles in somatic cells [3].
  • A homozygous deletion of 27 basepairs in the coding region of the human outer dense fiber protein gene does not result in a pathologic phenotype [4].
  • Outer dense fibers (ODF) are specific subcellular components of the sperm flagellum [5].
  • We have investigated the protein composition of purified ODF from bovine spermatozoa and found that one of the most abundant proteins is a 30-32-kDa polypeptide [5].

Biological context of ODF1


Anatomical context of ODF1


Associations of ODF1 with chemical compounds

  • The ODF1 leucine zipper and the KLC3 HR mediated the interaction [14].
  • For isolation of the outer dense fibers (ODF), the flagellar membrane and fibrous sheath were dissolved under reducing conditions in the cationic detergent cetyltrimethylammonium bromide (CTAB) for 30, 60 and 90 min, respectively [12].
  • The following parameters were determined on days 0 and 7: GFR and renal plasma flow (RPF) by 99mTc-DTPA and 131I-hippuran clearances; the extracellular volume, assimilated to the DTPA diffusion volume; urinary ODF by receptor-binding assay and urinary as well as plasma catecholamines by HPLC after extraction on alumin [15].
  • All the 27 cysteine residues in the ODF sequence except those in the C.X.P. repeat are conserved in the four species [16].

Physical interactions of ODF1


Regulatory relationships of ODF1


Other interactions of ODF1


Analytical, diagnostic and therapeutic context of ODF1

  • Here we demonstrate by independent cDNA isolations and by translational/immunoprecipitation of testicular mRNAs using anti-ODF 84 antibodies that 111-450 cDNA encodes the 84-kDa protein [8].
  • To analyze the molecular weight of in vitro-translated ODF27, we carried out gel filtration experiments [17].
  • For antibody screening an ELISA was developed using a newly developed method to fix the ODF proteins to the solid phase [19].
  • The mAbs recognize the major protein component of boar ODF on preparative Western blot and mark the mid- and principal piece of demembranated flagella [19].
  • Using RT7 system, a rat heart allograft model from RT7a donors to RT7b recipients was designed to test functional relevance of graft-associated hematopoietic cells (microchimerism) to allograft acceptance [20].


  1. Expression of the human antigen SPAG2 in the testis and localization to the outer dense fibers in spermatozoa. Diekman, A.B., Olson, G., Goldberg, E. Mol. Reprod. Dev. (1998) [Pubmed]
  2. RANK-L and RANK: T cells, bone loss, and mammalian evolution. Theill, L.E., Boyle, W.J., Penninger, J.M. Annu. Rev. Immunol. (2002) [Pubmed]
  3. Requirement of hCenexin for Proper Mitotic Functions of Polo-Like Kinase 1 at the Centrosomes. Soung, N.K., Kang, Y.H., Kim, K., Kamijo, K., Yoon, H., Seong, Y.S., Kuo, Y.L., Miki, T., Kim, S.R., Kuriyama, R., Giam, C.Z., Ahn, C.H., Lee, K.S. Mol. Cell. Biol. (2006) [Pubmed]
  4. A homozygous deletion of 27 basepairs in the coding region of the human outer dense fiber protein gene does not result in a pathologic phenotype. Hofferbert, S., Burfeind, P., Hoyer-Fender, S., Lange, R., Haidl, G., Engel, W. Hum. Mol. Genet. (1993) [Pubmed]
  5. Voltage-dependent anion-selective channels VDAC2 and VDAC3 are abundant proteins in bovine outer dense fibers, a cytoskeletal component of the sperm flagellum. Hinsch, K.D., De Pinto, V., Aires, V.A., Schneider, X., Messina, A., Hinsch, E. J. Biol. Chem. (2004) [Pubmed]
  6. Novel RING finger protein OIP1 binds to conserved amino acid repeats in sperm tail protein ODF1. Zarsky, H.A., Tarnasky, H.A., Cheng, M., van der Hoorn, F.A. Biol. Reprod. (2003) [Pubmed]
  7. Sequence, expression, and chromosomal assignment of a human sperm outer dense fiber gene. Gastmann, O., Burfeind, P., Günther, E., Hameister, H., Szpirer, C., Hoyer-Fender, S. Mol. Reprod. Dev. (1993) [Pubmed]
  8. Developmental expression of the 84-kDa ODF sperm protein: localization to both the cortex and medulla of outer dense fibers and to the connecting piece. Schalles, U., Shao, X., van der Hoorn, F.A., Oko, R. Dev. Biol. (1998) [Pubmed]
  9. A sperm cytoskeletal protein TSA70 is a novel phosphorylated member of Cenexin/odf2 family. Wakle, M., Khole, V. Biochem. Biophys. Res. Commun. (2007) [Pubmed]
  10. A novel testis-specific gene, SPAG4, whose product interacts specifically with outer dense fiber protein ODF27, maps to human chromosome 20q11.2. Tarnasky, H., Gill, D., Murthy, S., Shao, X., Demetrick, D.J., van der Hoorn, F.A. Cytogenet. Cell Genet. (1998) [Pubmed]
  11. Rat Spag5 associates in somatic cells with endoplasmic reticulum and microtubules but in spermatozoa with outer dense fibers. Fitzgerald, C.J., Oko, R.J., van der Hoorn, F.A. Mol. Reprod. Dev. (2006) [Pubmed]
  12. Isolation and partial characterization of the outer dense fiber proteins from human spermatozoa. Henkel, R., Stalf, T., Miska, W. Biol. Chem. Hoppe-Seyler (1992) [Pubmed]
  13. Outer dense fiber 2 is a widespread centrosome scaffold component preferentially associated with mother centrioles: its identification from isolated centrosomes. Nakagawa, Y., Yamane, Y., Okanoue, T., Tsukita, S., Tsukita, S. Mol. Biol. Cell (2001) [Pubmed]
  14. Association of kinesin light chain with outer dense fibers in a microtubule-independent fashion. Bhullar, B., Zhang, Y., Junco, A., Oko, R., van der Hoorn, F.A. J. Biol. Chem. (2003) [Pubmed]
  15. Natriuretic and vasoactive hormones and glomerular hyperfiltration in hyperglycaemic type 2 diabetic patients: effect of insulin treatment. Lalau, J.D., Westeel, P.F., Tenenbaum, F., Debussche, X., Nussberger, J., Tribout, B., Fardelonne, P., Favre, H., Fournier, A. Nephron (1993) [Pubmed]
  16. Molecular cloning and characterization of the bovine and porcine outer dense fibers cDNA and organization of the bovine gene. Kim, Y., Adham, I.M., Haack, T., Kremling, H., Engel, W. Biol. Chem. Hoppe-Seyler (1995) [Pubmed]
  17. Self-interaction of the major 27-kilodalton outer dense fiber protein is in part mediated by a leucine zipper domain in the rat. Shao, X., van der Hoorn, F.A. Biol. Reprod. (1996) [Pubmed]
  18. Interactional cloning of the 84-kDa major outer dense fiber protein Odf84. Leucine zippers mediate associations of Odf84 and Odf27. Shao, X., Tarnasky, H.A., Schalles, U., Oko, R., van der Hoorn, F.A. J. Biol. Chem. (1997) [Pubmed]
  19. Production and characterization of monoclonal antibodies to the major protein of boar outer dense fibers. Villegas, J., Henkel, R., Folch, H., Sánchez, R., Schill, W.B., Miska, W. J. Reprod. Immunol. (1998) [Pubmed]
  20. Manipulation of CD45 antigen in transplantation tolerance. Ko, S., Jaeger, M.D., Dahlke, M.H., Nakajima, Y., Schlitt, H.J. Curr. Mol. Med. (2002) [Pubmed]
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