Disease relevance of BDNF

• In addition, BrdU(+)/NeuN(+)-labeled cells developed in the hilar region after BDNF infusion, which have previously only been identified after severe continuous seizures (status epilepticus) and associated pathological changes [1].

High impact information on BDNF

• The resultant capacitative entry of calcium seems to be the actual trigger for BDNF-induced release of dopamine [2].
• PURPOSE: To determine whether BDNF modulates the release of dopamine from amacrine cells in the rabbit retina [2].
• Double-label immunohistochemistry was used to identify tyrosine hydroxylase containing neurons and to localize TrkB (BDNF) receptors [2].
• Antagonists of gamma-aminobutyric acid (GABA) and glutamate did not affect the BDNF-evoked release of dopamine [2].
• The reduction in dye transfer was not observed when the cells were exposed to epidermal growth factor or other neurotrophins, including neurotrophin 3, neurotrophin 4, and brain-derived neurotrophic factor [3].

Biological context of BDNF

• Neuronal precursors of the adult rat subependymal zone persist into senescence, with no decline in spatial extent or response to BDNF [4].
• In in vitro maturation experiments, the proportion of cumulus oocyte complexes maturing to metaphase II was not altered by BDNF in groups lacking fetal calf serum (FCS), but was significantly lower than the positive control containing 10% FCS (P < 0.01) [5].
• These techniques are applied to determining the degree of erythropoietin glycosylation, the degree of polyethylene glycol conjugation to RNase A and brain-derived neurotrophic factor, and the solution association states of these molecules [6].
• Brain-derived neurotrophic factor promotes bovine oocyte cytoplasmic competence for embryo development [5].

Anatomical context of BDNF

• However, mRNA for the BDNF-specific full length and truncated isoforms of the TrkB receptor are only detected in cumulus, suggesting that oocytes and cumulus differ in their capacity to respond to neurotrophin signalling [5].
• Maturation in the presence of a BDNF blocking antibody resulted in a blastocyst yield that was comparable to the absence of serum, and lower than in the presence of BDNF (P < 0.01) [5].
• The purpose of this study was to evaluate the effect of continuously released BDNF on peripheral nerve regeneration in a rat model [7].
• The results demonstrate that BDNF administration to the dentate gyrus leads to increased neurogenesis of granule cells [1].
• These results are discussed in light of the evidence that BDNF increases neuronal activity in hippocampus [1].

Other interactions of BDNF

• This method was applied to several fragments of platelet-derived growth factor B chain and brain-derived neurotrophic factor [8].

Analytical, diagnostic and therapeutic context of BDNF

• By RT-PCR and immunohistochemistry, cumulus and oocytes were shown to express mRNA and protein for BDNF and the p75 common neurotrophin receptor [5].
• Using quantitative anatomical techniques, we show that (i) all RGCs survived 1 week after a single injection of BDNF at the time of axotomy [9].
• The rats treated with BDNF (groups 2 and 3) demonstrated significantly less autocannibalization than the control group (group 1) [7].

References