Disease relevance of LST1

• These findings suggest that NCR3 and soluble isoforms of LST1 may play a role in inflammatory and infectious diseases [1].
• Highlighted genes such as SLA, LST1, and BENE might be important in the development of neural tube defects [2].

High impact information on LST1

• METHODS: A rat liver cDNA library was screened with human LST-1 cDNA as a probe [3].
• This region is known to encode the tumor necrosis factors (TNFs) alpha and beta, B144, and the major heat shock protein HSP70 [4].
• LST1: a gene with extensive alternative splicing and immunomodulatory function [5].
• We demonstrate the presence of the transmembrane variant LST1/C on the cell surface of the monocytic cell lines U937 and THP1 [5].
• These data demonstrate the protein expression of LST1 on the cell surface of mononuclear cells, and they show an inhibitory effect on lymphocyte proliferation of two LST1 proteins although they have only a very short amino acid homology [5].

Biological context of LST1

• Isolation of mouse LST1 cDNA clones established the open reading frame [6].
• The LST1 gene undergoes extensive alternative splicing, giving rise to both membrane-bound (encoded by exon 3) and soluble isoforms [1].
• In contrast, the alternative transmembrane variant LST1/A, the extracellular domain of which shows no amino acid sequence homology to LST1/C exerted a weaker but similar inhibitory effect on PBMC [5].
• The gene for the human leukocyte-specific transcript 1 (LST1) encodes a small protein that modulates immune responses and cellular morphogenesis [7].
• The leucocyte specific transcript - 1 (LST1) represents the human homolog of the mouse B144 transcript, encoded within the tumor necrosis factor (TNF) region of the human major histocompatibility complex class III interval [8].

Anatomical context of LST1

• Isolation of a full-length cDNA clone revealed that LST1 codes for IFN-gamma-inducible 800 nt transcripts, which are present in lymphoid tissues, T cells, macrophages, and histiocyte cell lines [8].
• Recombinant expression of LST1/C permitted its profound inhibitory effect on lymphocyte proliferation to be observed [5].
• The LST1 gene is constitutively expressed in leukocytes and dendritic cells, and it is characterized by extensive alternative splicing [5].
• STUDY DESIGN AND METHODS: Buffy coat-depleted red blood cells were filtered with an in-line filtration set (LCR5, MacoPharma) after 4 hours (LCR5/4 hr) and 16 hours (LCR5/16 hr) of storage, and whole blood was filtered with a whole-blood filtration set (LST1, MacoPharma [LST1/4 hr]) [9].
• Expression of LST-1 was immunohistochemically demonstrated in the human hepatocytes, but not in Hep G2 cells, which showed very low uptake of pravastatin [10].

Associations of LST1 with chemical compounds

• Its close linkage to the TNF genes and pattern of expression point toward a possible role for LST1 in the immune response [8].
• These data suggest that in humans, several different molecules are involved in transporting thyroid hormone: OATP in the brain, LST-1 in the liver, and OATP-E in peripheral tissues [11].

Other interactions of LST1

• Cloning and genomic characterization of LST1: a new gene in the human TNF region [8].
• Through a fine mapping using markers of the HLA central region a preferential recombination site was localized in the 60 kilobase interval between TNFd,e, and D6S273/K11 Amicrosatellite loci (i.e., between LST1 and BAT3 genes) [12].

Analytical, diagnostic and therapeutic context of LST1

• By use of LST1/4 hr no difference was found compared to LCR5/4 hr after filtration (0.12 +/- 0.09 vs. 0.12 +/- 0.1), but a significant difference was found when comparing the results before filtration (1.25 +/- 0.41 vs. 11.5 +/- 6.84; p < 0.02) [9].
• RT-PCR analysis showed that B144 is expressed in spleen, in thymus, and prominently in the macrophage cell line, U937 [13].

References