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Gene Review

CASP5  -  caspase 5, apoptosis-related cysteine...

Homo sapiens

Synonyms: CASP-5, Caspase-5, ICE(rel)-III, ICE(rel)III, ICEREL-III, ...
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Disease relevance of CASP5

  • However, secondary CD34+ liquid cultures reselected from the primary liquid cultures 24 hours after HIV exposure by panning with the ICH3 CD34 MoAb (ICH3/CD34+) and maintained for an additional 14 days were negative for HIV expression [1].

High impact information on CASP5

  • Pro-interleukin-1 beta processing activity could not be detected in cells transfected with ICErel-II or ICErel-III, but pro-domain-less truncated forms of ICErel-II and ICErel-III were capable of effectively inducing fibroblast apoptosis [2].
  • The CD34+ cell isolation scheme involved three sequential processes: (1) purification of bone marrow mononuclear cells; (2) enrichment of CD34+ cells using covalently immobilized soybean agglutinin; and (3) positive selection of CD34+ cells using polystyrene surfaces coated with the anti-CD34 monoclonal antibody ICH3 [3].
  • Sequential immunoprecipitation and Western blotting studies demonstrate that BI.3C5, ICH3, My10, and an antibody directed against endothelial cells, 188.27, all react with the same glycoprotein species, although the epitopes involved may be distinct [4].
  • Monoclonal antibodies My10, BI.3C5, 12.8, and ICH3 identify a monomeric cell surface glycoprotein (HPCA-1) of 100-120 kD, which is selectively expressed on human hemopoietic progenitor cells [4].
  • The epitope recognized by BI.3C5 is sialic acid dependent, whereas that recognized by ICH3 is not [4].

Biological context of CASP5

  • Interleukin-1beta-converting enzyme (ICE) and related cell death genes ICErel-II and ICErel-III map to the same PAC clone at band 11q22.2-22.3 [5].
  • This protocol is similar to the SAM-T02 protocol used in CASP5, but has improvements in the iterative search for similar sequences in finding and aligning templates, in creating fragment libraries, in generating protein conformations, and in scoring the conformations [6].
  • This report summarizes the Critical Assessment of Protein Structure Prediction (CASP5) target proteins, which included 67 experimental models submitted from various structural genomics efforts and independent research groups [7].
  • This combined method was used to make predictions for 28 protein domains in the Critical Assessment of Protein Structure 4 (CASP 4) and 59 domains in CASP 5, where the method ranked highly among comparative modeling and fold recognition methods [8].
  • Because of these cross-reactions, especially found with the anti-CD34 mAbs 12.8 and ICH3, we have demonstrated that there is a potential risk of cell harvest contamination by circulating neuroblastoma cells during CD34+ stem cell selection [9].

Associations of CASP5 with chemical compounds

  • CASP5 and CAFASP3 gave us the first chance to test RAPTOR in an unbiased way [10].
  • In normal resting tissues, anti-CD34 antibodies, ICH3 and QBEND-10 predominantly stain the luminal endothelial membrane, whereas the abluminal membrane is negative or weakly positive [11].

Other interactions of CASP5

  • The modeling approach used in CASP5 was similar to that used 2 years ago in CASP4 (Venclovas, Proteins 2001; Suppl 5:47-54) [12].
  • Mutations were detected in FAS (T=1, HY=1), BAX (T=6, HY=1), CASP5 (T=2) and IGFIIR (T=3, HY=1) genes [13].
  • We specifically addressed the validation of our simple approach for protein structure prediction through CASP6 because the fold recognition results of CASP5 revealed areas of improvement in the selection of good models [14].

Analytical, diagnostic and therapeutic context of CASP5

  • In a test set of CASP5 targets, SSALN outperforms sequence alignment methods such as a Smith-Waterman algorithm with BLOSUM50 and PSI_BLAST [15].
  • The ICH3-unbound cells were positive for both spliced and unspliced HIV RNA when exposed to HIV-1 Ba-L, and were DNA PCR positive when exposed to either monocytotropic or lymphotropic HIV-1 [1].


  1. Exposure of human CD34+ cells to human immunodeficiency virus type 1 does not influence their expansion and proliferation of hematopoietic progenitors in vitro. Kaushal, S., La Russa, V.F., Gartner, S., Kessler, S., Perfetto, S., Yu, Z., Ritchey, D.W., Xu, J., Perera, P., Kim, J., Reid, T., Mayers, D.L., St Louis, D., Mosca, J.D. Blood (1996) [Pubmed]
  2. Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases. Munday, N.A., Vaillancourt, J.P., Ali, A., Casano, F.J., Miller, D.K., Molineaux, S.M., Yamin, T.T., Yu, V.L., Nicholson, D.W. J. Biol. Chem. (1995) [Pubmed]
  3. Rapid isolation of human CD34 hematopoietic stem cells--purging of human tumor cells. Lebkowski, J.S., Schain, L.R., Okrongly, D., Levinsky, R., Harvey, M.J., Okarma, T.B. Transplantation (1992) [Pubmed]
  4. Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hemopoietic progenitor cells. Watt, S.M., Karhi, K., Gatter, K., Furley, A.J., Katz, F.E., Healy, L.E., Altass, L.J., Bradley, N.J., Sutherland, D.R., Levinsky, R. Leukemia (1987) [Pubmed]
  5. Interleukin-1beta-converting enzyme (ICE) and related cell death genes ICErel-II and ICErel-III map to the same PAC clone at band 11q22.2-22.3. Nasir, J., Theilmann, J.L., Vaillancourt, J.P., Munday, N.A., Ali, A., Scherer, S., Beatty, B., Nicholson, D.W., Hayden, M.R. Mamm. Genome (1997) [Pubmed]
  6. SAM-T04: what is new in protein-structure prediction for CASP6. Karplus, K., Katzman, S., Shackleford, G., Koeva, M., Draper, J., Barnes, B., Soriano, M., Hughey, R. Proteins (2005) [Pubmed]
  7. CASP5 target classification. Kinch, L.N., Qi, Y., Hubbard, T.J., Grishin, N.V. Proteins (2003) [Pubmed]
  8. Modeling structurally variable regions in homologous proteins with rosetta. Rohl, C.A., Strauss, C.E., Chivian, D., Baker, D. Proteins (2004) [Pubmed]
  9. Expression of CD34 and other haematopoietic antigens on neuroblastoma cells: consequences for autologous bone marrow and peripheral blood stem cell transplantation. Voigt, A., Häfer, R., Gruhn, B., Zintl, F. J. Neuroimmunol. (1997) [Pubmed]
  10. Assessment of RAPTOR's linear programming approach in CAFASP3. Xu, J., Li, M. Proteins (2003) [Pubmed]
  11. Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Schlingemann, R.O., Rietveld, F.J., de Waal, R.M., Bradley, N.J., Skene, A.I., Davies, A.J., Greaves, M.F., Denekamp, J., Ruiter, D.J. Lab. Invest. (1990) [Pubmed]
  12. Comparative modeling in CASP5: progress is evident, but alignment errors remain a significant hindrance. Venclovas, C. Proteins (2003) [Pubmed]
  13. Apoptotic and growth regulatory genes as mutational targets in mismatch repair deficient endometrioid adenocarcinomas of young patients. Vassileva, V., Millar, A., Briollais, L., Chapman, W., Bapat, B. Oncol. Rep. (2004) [Pubmed]
  14. Protein structure prediction using a variety of profile libraries and 3D verification. Tomii, K., Hirokawa, T., Motono, C. Proteins (2005) [Pubmed]
  15. SSALN: an alignment algorithm using structure-dependent substitution matrices and gap penalties learned from structurally aligned protein pairs. Qiu, J., Elber, R. Proteins (2006) [Pubmed]
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