The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

VPS15  -  Vps15p

Saccharomyces cerevisiae S288c

Synonyms: GRD8, Golgi-retention defective mutant protein 8, Serine/threonine-protein kinase VPS15, VAC4, VPL19, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on VPS15


Biological context of VPS15


Anatomical context of VPS15

  • VPS15 and VPS34 encode a serine/ threonine protein kinase and a phosphatidylinositol 3-kinase, respectively, that interact at the cytoplasmic face of an intracellular membrane compartment, most likely corresponding to the late Golgi [4].
  • A membrane-associated complex composed of the Vps15 protein kinase and the Vps34 phosphatidylinositol 3-kinase (PtdIns 3-kinase) is essential for the delivery of proteins to the yeast vacuole [5].
  • Fractionation of p2CPY accumulated in the temperature sensitive vps15 mutant revealed that the vps15 transport block did not occur in the endosome-like compartment but rather in the late Golgi complex, presumably the site of CPY sorting [8].

Associations of VPS15 with chemical compounds

  • Analysis of a temperature-conditional allele of VPS15, in which a shift to the nonpermissive temperature leads to a decrease in cellular PtdIns(3)P levels, indicates that the loss of Vps15p function leads to a defect in activation of Vps34p [5].
  • In methanol-grown cells of a P. pastoris VPS15 deletion strain, the levels of peroxisomal marker enzymes remained high after addition of excess glucose or ethanol [7].
  • Subcellular fractionation and sucrose density gradients indicate that Vps15p is responsible for the association of Vps34p with an intracellular membrane fraction [9].
  • Biochemical characterization of the autophosphorylation reaction catalyzed by Vps15p demonstrates that it is a functional serine/threonine protein kinase [10].

Regulatory relationships of VPS15

  • Consistent with this, mutations altering highly conserved residues in the lipid kinase domain of Vps34p lead to a dominant-negative phenotype resulting from titration of activating Vps15 proteins [5].

Other interactions of VPS15

  • Biochemical fractionation experiments have established that Ycf1p, expressed at single-copy gene levels, co-fractionates with the vacuolar membrane and that this co-fractionation is independent of vps15, vps34 or end3 gene function [11].

Analytical, diagnostic and therapeutic context of VPS15


  1. Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome. Slessareva, J.E., Routt, S.M., Temple, B., Bankaitis, V.A., Dohlman, H.G. Cell (2006) [Pubmed]
  2. Identification and mutation analysis of the complete gene for Chediak-Higashi syndrome. Nagle, D.L., Karim, M.A., Woolf, E.A., Holmgren, L., Bork, P., Misumi, D.J., McGrail, S.H., Dussault, B.J., Perou, C.M., Boissy, R.E., Duyk, G.M., Spritz, R.A., Moore, K.J. Nat. Genet. (1996) [Pubmed]
  3. A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole. Herman, P.K., Stack, J.H., DeModena, J.A., Emr, S.D. Cell (1991) [Pubmed]
  4. Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins. Stack, J.H., Horazdovsky, B., Emr, S.D. Annu. Rev. Cell Dev. Biol. (1995) [Pubmed]
  5. Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for protein sorting to the vacuole in yeast. Stack, J.H., DeWald, D.B., Takegawa, K., Emr, S.D. J. Cell Biol. (1995) [Pubmed]
  6. An essential role for a protein and lipid kinase complex in secretory protein sorting. Herman, P.K., Stack, J.H., Emr, S.D. Trends Cell Biol. (1992) [Pubmed]
  7. A Pichia pastoris VPS15 homologue is required in selective peroxisome autophagy. Stasyk, O.V., van der Klei, I.J., Bellu, A.R., Shen, S., Kiel, J.A., Cregg, J.M., Veenhuis, M. Curr. Genet. (1999) [Pubmed]
  8. Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment. Vida, T.A., Huyer, G., Emr, S.D. J. Cell Biol. (1993) [Pubmed]
  9. A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole. Stack, J.H., Herman, P.K., Schu, P.V., Emr, S.D. EMBO J. (1993) [Pubmed]
  10. Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. Stack, J.H., Emr, S.D. J. Biol. Chem. (1994) [Pubmed]
  11. Mutational analysis of the Saccharomyces cerevisiae ATP-binding cassette transporter protein Ycf1p. Wemmie, J.A., Moye-Rowley, W.S. Mol. Microbiol. (1997) [Pubmed]
  12. A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system. Volinia, S., Dhand, R., Vanhaesebroeck, B., MacDougall, L.K., Stein, R., Zvelebil, M.J., Domin, J., Panaretou, C., Waterfield, M.D. EMBO J. (1995) [Pubmed]
WikiGenes - Universities