The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

YPK1  -  Ypk1p

Saccharomyces cerevisiae S288c

Synonyms: SLI2, Serine/threonine-protein kinase YPK1, Sphingosine-like immunosuppressant resistant protein 2, YKL126W, Yeast protein kinase 1
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of YPK1

  • When overexpressed, a catalytically active C-terminal fragment (kinase domain) of Ypk1 was growth inhibitory; loss of Pkh1 (but not Pkh2) alleviated toxicity [1].

High impact information on YPK1

  • The threonine in Ypk1 that is phosphorylated by Pkh1 is required for efficient endocytosis, and pkh mutant cells are defective in alpha-factor internalization and fluid-phase endocytosis [2].
  • The kinase activity of Ypk1 is required for receptor endocytosis because mutations in two residues important for its catalytic activity cause a severe defect in alpha-factor internalization [2].
  • Ypk1 is required for both receptor-mediated and fluid-phase endocytosis, and is not necessary for receptor phosphorylation or ubiquitination [2].
  • In a screen to detect proteins involved in ubiquitin-dependent receptor internalization, we identified the sphingoid base-regulated serine-threonine kinase Ypk1 [2].
  • One of these genes, SLI2, is synonymous with YPK1, which encodes a serine/threonine kinase [3].

Biological context of YPK1


Anatomical context of YPK1

  • The major voltage-dependent ion channel in the plasma membrane of Saccharomyces cerevisiae, a conspicuous outwardly rectifying K+ channel, was first dubbed YPK1 and later renamed according to its registered gene names (DUK1, TOK1) [9].
  • The Pkh1 protein kinase of Saccharomyces cerevisiae, a homolog of the mammalian 3-phosphoinositide-dependent kinase (PDK1), regulates downstream AGC-type protein kinases including Ypk1/2 and Pkc1, which control cell wall integrity, growth, and other processes [10].

Associations of YPK1 with chemical compounds

  • In a previous study, we cloned yeast multicopy suppressor genes for ISP-1, and one of these, YPK1/SLI2, was shown to encode a serine/threonine kinase which is a yeast homologue of mammalian SGK1 (serum/glucocorticoid-regulated kinase 1) [6].
  • In addition, Ypk1 as well as eIF4G protein levels were rapidly depleted upon nitrogen starvation, but not during glucose starvation, even though both conditions inhibit translation initiation [11].

Regulatory relationships of YPK1


Other interactions of YPK1


Analytical, diagnostic and therapeutic context of YPK1

  • Sequence analysis revealed that MCK1 encodes a protein kinase homolog identical to YPK1, a phosphotyrosyl protein with demonstrated protein kinase activity [14].


  1. Pkh1 and pkh2 differentially phosphorylate and activate ypk1 and ykr2 and define protein kinase modules required for maintenance of cell wall integrity. Roelants, F.M., Torrance, P.D., Bezman, N., Thorner, J. Mol. Biol. Cell (2002) [Pubmed]
  2. The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast. deHart, A.K., Schnell, J.D., Allen, D.A., Hicke, L. J. Cell Biol. (2002) [Pubmed]
  3. Sli2 (Ypk1), a homologue of mammalian protein kinase SGK, is a downstream kinase in the sphingolipid-mediated signaling pathway of yeast. Sun, Y., Taniguchi, R., Tanoue, D., Yamaji, T., Takematsu, H., Mori, K., Fujita, T., Kawasaki, T., Kozutsumi, Y. Mol. Cell. Biol. (2000) [Pubmed]
  4. Mapping of DBR1 and YPK1 suggests a major revision of the genetic map of the left arm of Saccharomyces cerevisiae Chromosome XI. Simchen, G., Chapman, K.B., Caputo, E., Nam, K., Riles, L., Levin, D.E., Boeke, J.D. Genetics (1994) [Pubmed]
  5. The requirement for the hydrophobic motif phosphorylation of Ypk1 in yeast differs depending on the downstream events, including endocytosis, cell growth, and resistance to a sphingolipid biosynthesis inhibitor, ISP-1. Tanoue, D., Kobayashi, T., Sun, Y., Fujita, T., Takematsu, H., Kozutsumi, Y. Arch. Biochem. Biophys. (2005) [Pubmed]
  6. SLI1 (YGR212W) is a major gene conferring resistance to the sphingolipid biosynthesis inhibitor ISP-1, and encodes an ISP-1 N-acetyltransferase in yeast. Momoi, M., Tanoue, D., Sun, Y., Takematsu, H., Suzuki, Y., Suzuki, M., Suzuki, A., Fujita, T., Kozutsumi, Y. Biochem. J. (2004) [Pubmed]
  7. Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast. Casamayor, A., Torrance, P.D., Kobayashi, T., Thorner, J., Alessi, D.R. Curr. Biol. (1999) [Pubmed]
  8. A pair of putative protein kinase genes (YPK1 and YPK2) is required for cell growth in Saccharomyces cerevisiae. Chen, P., Lee, K.S., Levin, D.E. Mol. Gen. Genet. (1993) [Pubmed]
  9. Physiological characterization of the yeast plasma membrane outward rectifying K+ channel, DUK1 (TOK1), in situ. Bertl, A., Bihler, H., Reid, J.D., Kettner, C., Slayman, C.L. J. Membr. Biol. (1998) [Pubmed]
  10. The sphingoid long chain base phytosphingosine activates AGC-type protein kinases in Saccharomyces cerevisiae including Ypk1, Ypk2, and Sch9. Liu, K., Zhang, X., Lester, R.L., Dickson, R.C. J. Biol. Chem. (2005) [Pubmed]
  11. Loss of ypk1 function causes rapamycin sensitivity, inhibition of translation initiation and synthetic lethality in 14-3-3-deficient yeast. Gelperin, D., Horton, L., DeChant, A., Hensold, J., Lemmon, S.K. Genetics (2002) [Pubmed]
  12. A protein kinase-encoding gene, pkt1, from Trichoderma reesei, homologous to the yeast YPK1 and YPK2 (YKR2) genes. Morawetz, R., Mischak, H., Goodnight, J., Lendenfeld, T., Mushinsky, J.F., Kubicek, C.P. Gene (1994) [Pubmed]
  13. Differential roles of PDK1- and PDK2-phosphorylation sites in the yeast AGC kinases Ypk1, Pkc1 and Sch9. Roelants, F.M., Torrance, P.D., Thorner, J. Microbiology (Reading, Engl.) (2004) [Pubmed]
  14. The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression. Neigeborn, L., Mitchell, A.P. Genes Dev. (1991) [Pubmed]
WikiGenes - Universities