Disease relevance of SNORD14B

• Comparison of the mouse U14 snRNA sequence with the U14 DNA sequences contained in the three mouse hsc70 introns indicates that intron 5 is utilized for U14 snRNA synthesis in normally growing mouse ascites cells [1].
• A single genomic fragment of mouse DNA containing the U14 snRNA gene(s) has been isolated from a Charon 4A lambda phage mouse genomic library and sequenced [1].
• Human herpesvirus 6 open reading frame U14 protein and cellular p53 interact with each other and are contained in the virion [2].
• The aim of present study is to determine whether the combined administration of hyaluronic acid (HA) with paclitaxel can produce additional or synergistic therapeutic effects in the control of Lewis lung carcinoma (LLC) migration and ascites formation of U14 cervical tumor [3].

High impact information on SNORD14B

• Intron binding protein (IBP) 160, a helicase-like protein previously detected in the spliceosomal C1 complex, binds the pre-mRNA in a sequence-independent manner, contacting nucleotides 33-40 upstream of the intron branch site, regardless of whether a snoRNA is present [4].
• In the spliceosomal C1 complex, snoRNP proteins efficiently assemble onto snoRNA sequences if they are located about 50 nt upstream of the intron branchpoint [4].
• The results indicate that the Has1p helicase is required for snoRNA release from pre-rRNA and production of the U6 snRNP [5].
• Point mutations in ATPase and helicase motifs of Has1p block U14 release from pre-rRNA [5].
• The Helicase Has1p Is Required for snoRNA Release from Pre-rRNA [5].

Biological context of SNORD14B

• We have now established an in vitro U14 snoRNP assembly system to characterize protein binding [6].
• Mutagenesis of either box C or box D completely blocked U14 processing [7].
• Processing of U14.6 from hsc70 intron 6 is not dependent upon the base pairing of intron sequences flanking the 5' and 3' termini of the encoded U14 snRNA molecule [8].
• The striking feature is that unlike its human and Xenopus counterparts, which encode two U14 snoRNAs in two separate introns, the hamster S13 gene encodes no U14 snoRNA [9].
• Despite Northern blotting evidence that U14 follows late kinetics, the U14 protein was detected immediately after infection (at 3 hpi) by IFA [2].

Anatomical context of SNORD14B

• Essential elements for intronic U14 processing have been analyzed by microinjecting various mutant hsc70/Ul4 pre-mRNA precursors into Xenopus oocyte nuclei [7].

Associations of SNORD14B with chemical compounds

• In addition, by Western blotting, U14 was detected at 0 hpi or in the presence of cycloheximide which completely abolished the expression of IE1 protein [2].
• Ethanol at concentrations of 0.5%, 0.25% and 0.125% had no effect on the incorporation of U14 C-glucose into lipid [10].

Analytical, diagnostic and therapeutic context of SNORD14B

• Indirect immunofluorescence assays (IFAs) showed that by 18 h postinfection (hpi) U14 localized to the dot-like structures observed in both the nucleus and cytoplasm where p53 was partly accumulated [2].
• METHODS: Anthropometry, maturity status, functional and sport-specific parameters were assessed in elite, sub-elite, and non-elite youth players in four age groups: U13 (n = 117), U14 (n = 136), U15 (n = 138) and U16 (n = 99) [11].

References