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Gene Review

alphaTub84B  -  alpha-Tubulin at 84B

Drosophila melanogaster

Synonyms: 1t, ALPHA 84C, BEST:LD32507, CG1913, DM1alpha, ...
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Disease relevance of alphaTub84B

  • The dominant male sterility of the revertant alleles is suppressed if the flies are also heterozygous for B2tn, for a deficiency of alpha 1t, or for the haync2 allele [1].
  • A lambda phage clone containing alpha 1-tubulin specific sequences was isolated from a genomic DNA library of Drosophila auraria and its restriction endonuclease pattern is presented [2].

Psychiatry related information on alphaTub84B

  • The polarity of kinesin's motor activity can be reversed by MT disassembly and interactions between a motor and a MT end can either slow or speed the rate of tubulin depolymerization [3].

High impact information on alphaTub84B


Biological context of alphaTub84B


Anatomical context of alphaTub84B

  • We used transgenic analysis in Drosophila to compare the ability of two structurally similar alpha-tubulin isoforms to support microtubule assembly in vivo [7].
  • In spermatogenesis, all microtubule functions in the postmitotic male germ cells are carried out by a single tubulin heterodimer composed of the major Drosophila alpha-84B tubulin isoform and the testis-specific beta 2-tubulin isoform [7].
  • Although alpha4-tubulin comprises only about one-fifth of the alpha-tubulin pool in every Drosophila egg, in the absence of alpha4-tubulin - in eggs of the kavar(0)/- hemizygous females - only a tassel of short microtubules forms with two barely separated daughter centrosomes [10].
  • Thus, both classes of alpha-tubulin isotype present in the mature oocyte, alpha TUB67C and alpha TUB84B/84D, are essential for normal spindle function in early Drosophila development [11].
  • An involvement of polyglycylated tubulin in axoneme motility is suggested since AXO 49 and TAP 952 specifically inhibit the reactivated motility of sea urchin spermatozoa [12].

Associations of alphaTub84B with chemical compounds

  • We show that this second-site noncomplementing mutation is a missense mutation in alpha-tubulin that results in substitution of methionine in place of valine at amino acid 177 [13].
  • P4 is precipitated with vinblastine sulfate and migrates as a tubulin subunit [14].
  • Drosophila microsomes exhibited tocopherol-omega-hydroxylase activity in the order of delta-T > gamma-T >> alpha-T, a pattern consistent with the effect of PBO in vivo [15].

Physical interactions of alphaTub84B


Regulatory relationships of alphaTub84B

  • Fusion of ftz to the Tubalpha1 promoter resulted in low-level ectopic expression of FTZ relative to FTZ expressed from the endogenous ftz gene [17].
  • However, HOXA-2 expressed from a Tubulin alpha 1 promoter was unable to direct maxillary palp determination when either ectopically expressed in the antenna or in the maxillary palp primordia of a pb null mutant [18].

Other interactions of alphaTub84B

  • Two of the genes, alpha 1 and alpha 3, are constitutively expressed and code for proteins that are very similar to previously sequenced alpha-tubulins [19].

Analytical, diagnostic and therapeutic context of alphaTub84B

  • Western blots of 1D gels of proteins from epidermis, limb buds, or claw muscle showed tissue- and stage-specific changes in tubulin content; similar analyses on blots of 2D gels revealed differences in the number of alpha-tubulin isoforms that were expressed [8].
  • In immunofluorescence observations with antibodies against alpha-tubulin, the microtubules appear to radiate from two bright foci widely separated from each other [20].


  1. Two types of genetic interaction implicate the whirligig gene of Drosophila melanogaster in microtubule organization in the flagellar axoneme. Green, L.L., Wolf, N., McDonald, K.L., Fuller, M.T. Genetics (1990) [Pubmed]
  2. The organization of the alpha-tubulin gene family in the Drosophila montium subgroup of the melanogaster species group. Drosopoulou, E., Scouras, Z.G. Genome (1998) [Pubmed]
  3. Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization. Lombillo, V.A., Stewart, R.J., McIntosh, J.R. Nature (1995) [Pubmed]
  4. Apical localization of pair-rule transcripts requires 3' sequences and limits protein diffusion in the Drosophila blastoderm embryo. Davis, I., Ish-Horowicz, D. Cell (1991) [Pubmed]
  5. Regulation of tubulin gene expression during embryogenesis in Drosophila melanogaster. Raff, E.C., Fuller, M.T., Kaufman, T.C., Kemphues, K.J., Rudolph, J.E., Raff, R.A. Cell (1982) [Pubmed]
  6. alpha-Tubulin genes of Drosophila. Kalfayan, L., Wensink, P.C. Cell (1981) [Pubmed]
  7. Structurally similar Drosophila alpha-tubulins are functionally distinct in vivo. Hutchens, J.A., Hoyle, H.D., Turner, F.R., Raff, E.C. Mol. Biol. Cell (1997) [Pubmed]
  8. Molecular characterization of four members of the alpha-tubulin gene family of the Bermuda land crab Gecarcinus lateralis. Varadaraj, K., Kumari, S.S., Skinner, D.M. J. Exp. Zool. (1997) [Pubmed]
  9. Drosophila CLASP is required for the incorporation of microtubule subunits into fluxing kinetochore fibres. Maiato, H., Khodjakov, A., Rieder, C.L. Nat. Cell Biol. (2005) [Pubmed]
  10. alpha4-Tubulin is involved in rapid formation of long microtubules to push apart the daughter centrosomes during earlyx Drosophila embryogenesis. Venkei, Z., Gáspár, I., Tóth, G., Szabad, J. J. Cell. Sci. (2006) [Pubmed]
  11. A functionally specialized alpha-tubulin is required for oocyte meiosis and cleavage mitoses in Drosophila. Matthews, K.A., Rees, D., Kaufman, T.C. Development (1993) [Pubmed]
  12. Axonemal tubulin polyglycylation probed with two monoclonal antibodies: widespread evolutionary distribution, appearance during spermatozoan maturation and possible function in motility. Bré, M.H., Redeker, V., Quibell, M., Darmanaden-Delorme, J., Bressac, C., Cosson, J., Huitorel, P., Schmitter, J.M., Rossler, J., Johnson, T., Adoutte, A., Levilliers, N. J. Cell. Sci. (1996) [Pubmed]
  13. Interacting proteins identified by genetic interactions: a missense mutation in alpha-tubulin fails to complement alleles of the testis-specific beta-tubulin gene of Drosophila melanogaster. Hays, T.S., Deuring, R., Robertson, B., Prout, M., Fuller, M.T. Mol. Cell. Biol. (1989) [Pubmed]
  14. Expression of a new beta tubulin subunit is induced by 20-hydroxyecdysone in Drosophila cultured cells. Sobrier, M.L., Couderc, J.L., Chapel, S., Dastugue, B. Biochem. Biophys. Res. Commun. (1986) [Pubmed]
  15. Selective accumulation of alpha-tocopherol in Drosophila is associated with cytochrome P450 tocopherol-omega-hydroxylase activity but not alpha-tocopherol transfer protein. Parker, R.S., McCormick, C.C. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
  16. GAGA factor and TBF1 bind DNA elements that direct ubiquitous transcription of the Drosophila alpha 1-tubulin gene. O'Donnell, K.H., Wensink, P.C. Nucleic Acids Res. (1994) [Pubmed]
  17. Low-level ectopic expression of Fushi tarazu in Drosophila melanogaster results in ftz(Ual/Rpl)-like phenotypes and rescues ftz phenotypes. Argiropoulos, B., Ho, J., Blachuta, B.J., Tayyab, I., Percival-Smith, A. Mech. Dev. (2003) [Pubmed]
  18. Analysis of murine HOXA-2 activity in Drosophila melanogaster. Percival-Smith, A., Bondy, J.A. Dev. Genet. (1999) [Pubmed]
  19. Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins. Theurkauf, W.E., Baum, H., Bo, J., Wensink, P.C. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  20. Centrosome splitting during nuclear elongation in the Drosophila embryo. Callaini, G., Anselmi, F. Exp. Cell Res. (1988) [Pubmed]
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