Disease relevance of NHP6A

• In addition, expression of NHP6A in mutant Escherichia coli cells lacking HU and Fis restores normal morphological appearance to these cells, specifically in nucleoid condensation and segregation [1].

High impact information on NHP6A

• In vitro, NHP6A stimulated transcription by pol II at the GAL1 promoter three- to fivefold above the level of activation by GAL4-VP16 alone [2].
• Gel mobility shift assays showed that NHP6A promotes the formation of a complex with TBP and TFIIA at the TATA box that has enhanced affinity for TFIIB [2].
• Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro [2].
• Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B [3].
• We found that NHP6A/B proteins are also required for full induction of the yeast CHA1 gene [3].

Biological context of NHP6A

• From these data we predict diverse architectural roles for NHP6A/B in manipulating chromosome structure and promoting the assembly of multicomponent protein.DNA complexes [1].
• In this report, synthetic oligonucleotides, designed from the limited NHP6 amino acid sequence, were used as hybridization probes to clone the NHP6A gene from a yeast genomic library [4].
• Nucleotide sequence analysis revealed that NHP6B has six extra amino acids at its amino terminus, but that NHP6A and NHP6B match at 87% of the rest of their sequences [4].
• We therefore observe that protein transport along DNA by direct transfers occurs even for proteins such as NHP6A and HU that have only one DNA-binding domain [5].
• Changes in the NHP6B gene copy number caused more moderate changes in NHP6A synthesis [6].

Associations of NHP6A with chemical compounds

• Moreover, because overexpression of NHP6A can suppress caffeine sensitivity of one of the SWI6 ANK mutants, swi6-405, other SWI6-dependent genes may also be affected by Nhp6A [7].
• Methionine 29, which may intercalate into DNA, is essential for NHP6A-induced microcircle formation of 75-bp but not 98-bp fragments in vitro, and for full growth complementation of Deltanhp6a/b mutants in vivo [8].

Regulatory relationships of NHP6A

• The MSN1 and NHP6A genes suppress SWI6 defects in Saccharomyces cerevisiae [7].

Other interactions of NHP6A

• We found that MSN1, which encodes a transcriptional activator, and NHP6A, which encodes an HMG-like protein, are able to suppress defective Swi6 function [7].
• Mutations in POB3, but not in NHP6A/B, also display strong synthetic defects with hir/hpc mutations [9].
• Using a genetic screen for positive regulators able to compensate for a deficiency in a promoter element of the SNR6 gene, we isolated the NHP6A and NHP6B genes [10].
• Genes which in multiple copies suppress the synthetic lethality of slk1-1 spa2-delta were identified, and one, the NHP6A gene, has been extensively characterized [11].
• The NHP6A gene and the closely related NHP6B gene were shown previously to encode HMG1-like chromatin-associated proteins [11].

Analytical, diagnostic and therapeutic context of NHP6A

• Low stringency Southern blot analysis showed that there was a second gene homologous to NHP6A [4].
• The mechanical response generated by binding of the nonspecific DNA-bending proteins HMGB1, NHP6A, and HU to single tethered 48.5 kb lambda-DNA molecules is investigated using DNA micromanipulation [5].
• The effects of altering NHP6A or NHP6B gene number on the expression of its partner has been examined using Northern blots and reporter genes [6].

References