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Gene Review

NHP6A  -  Nhp6ap

Saccharomyces cerevisiae S288c

Synonyms: NHPA, Non-histone chromosomal protein 6A, YP9499.09C, YPR052C
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Disease relevance of NHP6A

  • In addition, expression of NHP6A in mutant Escherichia coli cells lacking HU and Fis restores normal morphological appearance to these cells, specifically in nucleoid condensation and segregation [1].

High impact information on NHP6A

  • In vitro, NHP6A stimulated transcription by pol II at the GAL1 promoter three- to fivefold above the level of activation by GAL4-VP16 alone [2].
  • Gel mobility shift assays showed that NHP6A promotes the formation of a complex with TBP and TFIIA at the TATA box that has enhanced affinity for TFIIB [2].
  • Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro [2].
  • Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B [3].
  • We found that NHP6A/B proteins are also required for full induction of the yeast CHA1 gene [3].

Biological context of NHP6A

  • From these data we predict diverse architectural roles for NHP6A/B in manipulating chromosome structure and promoting the assembly of multicomponent protein.DNA complexes [1].
  • In this report, synthetic oligonucleotides, designed from the limited NHP6 amino acid sequence, were used as hybridization probes to clone the NHP6A gene from a yeast genomic library [4].
  • Nucleotide sequence analysis revealed that NHP6B has six extra amino acids at its amino terminus, but that NHP6A and NHP6B match at 87% of the rest of their sequences [4].
  • We therefore observe that protein transport along DNA by direct transfers occurs even for proteins such as NHP6A and HU that have only one DNA-binding domain [5].
  • Changes in the NHP6B gene copy number caused more moderate changes in NHP6A synthesis [6].

Associations of NHP6A with chemical compounds

  • Moreover, because overexpression of NHP6A can suppress caffeine sensitivity of one of the SWI6 ANK mutants, swi6-405, other SWI6-dependent genes may also be affected by Nhp6A [7].
  • Methionine 29, which may intercalate into DNA, is essential for NHP6A-induced microcircle formation of 75-bp but not 98-bp fragments in vitro, and for full growth complementation of Deltanhp6a/b mutants in vivo [8].

Regulatory relationships of NHP6A


Other interactions of NHP6A

  • We found that MSN1, which encodes a transcriptional activator, and NHP6A, which encodes an HMG-like protein, are able to suppress defective Swi6 function [7].
  • Mutations in POB3, but not in NHP6A/B, also display strong synthetic defects with hir/hpc mutations [9].
  • Using a genetic screen for positive regulators able to compensate for a deficiency in a promoter element of the SNR6 gene, we isolated the NHP6A and NHP6B genes [10].
  • Genes which in multiple copies suppress the synthetic lethality of slk1-1 spa2-delta were identified, and one, the NHP6A gene, has been extensively characterized [11].
  • The NHP6A gene and the closely related NHP6B gene were shown previously to encode HMG1-like chromatin-associated proteins [11].

Analytical, diagnostic and therapeutic context of NHP6A


  1. DNA looping by Saccharomyces cerevisiae high mobility group proteins NHP6A/B. Consequences for nucleoprotein complex assembly and chromatin condensation. Paull, T.T., Johnson, R.C. J. Biol. Chem. (1995) [Pubmed]
  2. Yeast HMG proteins NHP6A/B potentiate promoter-specific transcriptional activation in vivo and assembly of preinitiation complexes in vitro. Paull, T.T., Carey, M., Johnson, R.C. Genes Dev. (1996) [Pubmed]
  3. Chromatin-mediated transcriptional regulation by the yeast architectural factors NHP6A and NHP6B. Moreira, J.M., Holmberg, S. EMBO J. (2000) [Pubmed]
  4. Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1. Kolodrubetz, D., Burgum, A. J. Biol. Chem. (1990) [Pubmed]
  5. Micromechanical analysis of the binding of DNA-bending proteins HMGB1, NHP6A, and HU reveals their ability to form highly stable DNA-protein complexes. Skoko, D., Wong, B., Johnson, R.C., Marko, J.F. Biochemistry (2004) [Pubmed]
  6. Gene dosage affects the expression of the duplicated NHP6 genes of Saccharomyces cerevisiae. Kolodrubetz, D., Kruppa, M., Burgum, A. Gene (2001) [Pubmed]
  7. The MSN1 and NHP6A genes suppress SWI6 defects in Saccharomyces cerevisiae. Sidorova, J., Breeden, L. Genetics (1999) [Pubmed]
  8. Determinants of DNA binding and bending by the Saccharomyces cerevisiae high mobility group protein NHP6A that are important for its biological activities. Role of the unique N terminus and putative intercalating methionine. Yen, Y.M., Wong, B., Johnson, R.C. J. Biol. Chem. (1998) [Pubmed]
  9. Defects in SPT16 or POB3 (yFACT) in Saccharomyces cerevisiae cause dependence on the Hir/Hpc pathway: polymerase passage may degrade chromatin structure. Formosa, T., Ruone, S., Adams, M.D., Olsen, A.E., Eriksson, P., Yu, Y., Rhoades, A.R., Kaufman, P.D., Stillman, D.J. Genetics (2002) [Pubmed]
  10. High-mobility-group proteins NHP6A and NHP6B participate in activation of the RNA polymerase III SNR6 gene. Lopez, S., Livingstone-Zatchej, M., Jourdain, S., Thoma, F., Sentenac, A., Marsolier, M.C. Mol. Cell. Biol. (2001) [Pubmed]
  11. NHP6A and NHP6B, which encode HMG1-like proteins, are candidates for downstream components of the yeast SLT2 mitogen-activated protein kinase pathway. Costigan, C., Kolodrubetz, D., Snyder, M. Mol. Cell. Biol. (1994) [Pubmed]
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