The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Micromanipulation

  • METHODS: We used complementarity-determining region 3 (CDR3) of the immunoglobulin heavy-chain gene as a clonal marker to study individual L&H cells isolated by micromanipulation from tissue sections of five patients with nodular lymphocyte-predominant Hodgkin's disease [1].
  • CONCLUSIONS: These data demonstrate that by the modification of standard laboratory methods for IVF, monospermic fertilization, cleavage, and a high clinical PR can be achieved in cases of severe male factor infertility without having to resort to micromanipulation [2].

High impact information on Micromanipulation


Biological context of Micromanipulation


Anatomical context of Micromanipulation


Associations of Micromanipulation with chemical compounds

  • The stability of this complex is investigated here by using two concordant nanotechniques (surface forces apparatus and vesicle micromanipulation) that allow direct measurements of adhesion energies due to the chelation of nickel ions by nitrilotriacetate (NTA) groups grafted on surfaces [17].
  • This simple method of gap construction requires no micromanipulation under a microscope, hydrofluoric acid etching, or use of column fittings [18].
  • Micromanipulation methods were used to select and transfer single lipid vesicles (2-3 X 10(-4) cm diameter) from a chamber that contained the vesicle suspension to a separate chamber on the microscope stage that contained red cells in an EDTA buffer with Dextran or whole plasma [19].
  • The main objectives of this study were to obtain clones of Paracoccidioides brasiliensis by two methods (micromanipulation and plating assay) and to determine if the secretion of the 43-kDa glycoprotein (gp43) is dependent on the clonal culture [20].
  • Only 1 of 69 eggs was damaged when sucrose was used to shrink the ooplasm during micromanipulation [21].

Gene context of Micromanipulation

  • Effects of the combination of ML and SF on multipotential progenitors were not mediated through other cells, as demonstrated by micromanipulation of individual progenitors [22].
  • Highly enriched murine marrow cells were plated individually in culture by micromanipulation in the presence of pokeweed mitogen-stimulated spleen cell conditioned medium, erythropoietin, steel factor (SF), and interleukin (IL) 7 [23].
  • Lin- Ly-6A/E+ marrow cells from 5-fluorouracil-treated mice were plated individually by micromanipulation in methylcellulose culture with steel factor (SF) and IL-11 for 8 days [24].
  • The mechanical response generated by binding of the nonspecific DNA-bending proteins HMGB1, NHP6A, and HU to single tethered 48.5 kb lambda-DNA molecules is investigated using DNA micromanipulation [25].
  • Direct measurement of CSF production by single Con A-pretreated cells isolated by micromanipulation revealed that 10 to 20% could secrete detectable CSF [26].

Analytical, diagnostic and therapeutic context of Micromanipulation


  1. Clonality in nodular lymphocyte-predominant Hodgkin's disease. Ohno, T., Stribley, J.A., Wu, G., Hinrichs, S.H., Weisenburger, D.D., Chan, W.C. N. Engl. J. Med. (1997) [Pubmed]
  2. Can severe male factor infertility be treated without micromanipulation? Ord, T., Patrizio, P., Balmaceda, J.P., Asch, R.H. Fertil. Steril. (1993) [Pubmed]
  3. Single-molecule analysis of DNA uncoiling by a type II topoisomerase. Strick, T.R., Croquette, V., Bensimon, D. Nature (2000) [Pubmed]
  4. Erbium YAG laser for micromanipulation of oocytes and spermatozoa. Feichtinger, W., Strohmer, H., Radner, K.M. Lancet (1992) [Pubmed]
  5. Micromanipulation of adhesion of a Jurkat cell to a planar bilayer membrane containing lymphocyte function-associated antigen 3 molecules. Tözeren, A., Sung, K.L., Sung, L.A., Dustin, M.L., Chan, P.Y., Springer, T.A., Chien, S. J. Cell Biol. (1992) [Pubmed]
  6. Control mechanisms of the cell cycle: role of the spatial arrangement of spindle components in the timing of mitotic events. Sluder, G., Begg, D.A. J. Cell Biol. (1983) [Pubmed]
  7. Role of cortical N-methyl-D-aspartate receptors in auditory sensory memory and mismatch negativity generation: implications for schizophrenia. Javitt, D.C., Steinschneider, M., Schroeder, C.E., Arezzo, J.C. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  8. Adhesiveness of human ligament fibroblasts to laminin. Sung, K.L., Steele, L.L., Whittermore, D., Hagan, J., Akeson, W.H. J. Orthop. Res. (1995) [Pubmed]
  9. Mussel MAP, a major gonad-duct esterase-like protein, is released into sea water as a dual constituent of the seminal fluid and the spermatozoon. Torrado, M., Paz, M., Korochkin, L.I., Mikhailov, A.T. J. Exp. Biol. (2003) [Pubmed]
  10. Human zona pellucida micromanipulation and monozygotic twinning frequency after IVF. Sills, E.S., Moomjy, M., Zaninovic, N., Veeck, L.L., McGee, M., Palermo, G.D., Rosenwaks, Z. Hum. Reprod. (2000) [Pubmed]
  11. Sperm stimulants can improve fertilization rates in male-factor cases undergoing IVF to the same extent as micromanipulation by partial zona dissection (PZD) or subzonal sperm insemination (SUZI): a randomized controlled study. Edirisinghe, W.R., Junk, S., Yovich, J.M., Bootsma, B., Yovich, J.L. J. Assist. Reprod. Genet. (1995) [Pubmed]
  12. Disparate differentiation in mouse hemopoietic colonies derived from paired progenitors. Suda, T., Suda, J., Ogawa, M. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  13. Production of two hemopoietic growth factors is differentially regulated in single T lymphocytes activated with an anti-T cell receptor antibody. Kelso, A., Owens, T. J. Immunol. (1988) [Pubmed]
  14. The B lymphocyte in rheumatoid arthritis: analysis of rearranged V kappa genes from B cells infiltrating the synovial membrane. Gause, A., Gundlach, K., Zdichavsky, M., Jacobs, G., Koch, B., Hopf, T., Pfreundschuh, M. Eur. J. Immunol. (1995) [Pubmed]
  15. Classical and alloimmune anaphylactic degranulation of isolated single mast cells. Thiernesse, N., Jeannesson, P., Bernard, J., Zagury, D., Voisin, G.A. J. Immunol. Methods (1978) [Pubmed]
  16. Epididymal micropuncture with in vitro fertilization and oocyte micromanipulation for the treatment of unreconstructable obstructive azoospermia. Schlegel, P.N., Berkeley, A.S., Goldstein, M., Cohen, J., Alikani, M., Adler, A., Gilbert, B.R., Rosenwaks, Z. Fertil. Steril. (1994) [Pubmed]
  17. The binding energy of two nitrilotriacetate groups sharing a nickel ion. Tareste, D., Pincet, F., Brellier, M., Mioskowski, C., Perez, E. J. Am. Chem. Soc. (2005) [Pubmed]
  18. Laser ablation construction of on-column reagent addition devices for capillary electrophoresis. Rezenom, Y.H., Lancaster, J.M., Pittman, J.L., Gilman, S.D. Anal. Chem. (2002) [Pubmed]
  19. Free energy potential for aggregation of erythrocytes and phosphatidylcholine/phosphatidylserine vesicles in Dextran (36,500 MW) solutions and in plasma. Evans, E., Kukan, B. Biophys. J. (1983) [Pubmed]
  20. Variable gp43 secretion by Paracoccidioides brasiliensis clones obtained by two different culture methods. Berzaghi, R., da Silva, S.H., de Camargo, Z.P. J. Clin. Microbiol. (2005) [Pubmed]
  21. Partial zona dissection of human oocytes when failure of zona pellucida penetration is anticipated. Cohen, J., Malter, H., Wright, G., Kort, H., Massey, J., Mitchell, D. Hum. Reprod. (1989) [Pubmed]
  22. Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation of primitive hematopoietic progenitors of mice. Ku, H., Yonemura, Y., Kaushansky, K., Ogawa, M. Blood (1996) [Pubmed]
  23. Clonal proliferation of murine lymphohemopoietic progenitors in culture. Hirayama, F., Shih, J.P., Awgulewitsch, A., Warr, G.W., Clark, S.C., Ogawa, M. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  24. Negative regulation of early T lymphopoiesis by interleukin-3 and interleukin-1 alpha. Hirayama, F., Ogawa, M. Blood (1995) [Pubmed]
  25. Micromechanical analysis of the binding of DNA-bending proteins HMGB1, NHP6A, and HU reveals their ability to form highly stable DNA-protein complexes. Skoko, D., Wong, B., Johnson, R.C., Marko, J.F. Biochemistry (2004) [Pubmed]
  26. An assay for colony-stimulating factor (CSF) production by single T lymphocytes: estimation of the frequency of cells producing granulocyte-macrophage CSF and multi-lineage CSF within a T lymphocyte clone. Kelso, A. J. Immunol. (1986) [Pubmed]
  27. Nuclear transfer of porcine embryos using cryopreserved delipated blastomeres as donor nuclei. Nagashima, H., Ashman, R.J., Nottle, M.B. Mol. Reprod. Dev. (1997) [Pubmed]
  28. Effect of polymerization conditions on the network properties of dex-HEMA microspheres and macro-hydrogels. Chung, J.T., Vlugt-Wensink, K.D., Hennink, W.E., Zhang, Z. International journal of pharmaceutics. (2005) [Pubmed]
  29. In vitro fertilization for male infertility: when and how? Hall, J., Fishel, S. Baillière's clinical obstetrics and gynaecology. (1997) [Pubmed]
  30. Assisted hatching in an in vitro fertilization programme. Parikh, F.R., Kamat, S.A., Nadkarni, S., Arawandekar, D., Parikh, R.M. J. Reprod. Fertil. Suppl. (1996) [Pubmed]
WikiGenes - Universities