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Gene Review

tonB  -  membrane spanning protein in TonB-ExbB...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1246, JW5195, T1rec, exbA
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Disease relevance of tonB


High impact information on tonB


Chemical compound and disease context of tonB


Biological context of tonB


Associations of tonB with chemical compounds

  • The tonB mutation with a glutamine to leucine replacement at position 165 was less efficient in restoring the FhuA functions than the glutamine to lysine exchange at the same position [15].
  • Mode of action of GR69153, a novel catechol-substituted cephalosporin, and its interaction with the tonB-dependent iron transport system [16].
  • Like tonB mutants, exb mutants were resistant to colicin M and albomycin but, in contrast to tonB mutants, showed only reduced sensitivity to colicins B and D. Overexpressed tonB on the multicopy vector pACYC177 largely restored the sensitivity of exb mutants to colicins B, D, and M but only marginally increased sensitivity to albomycin [17].
  • N. gonorrhoeae FA1090 utilized ferric enterobactin as the sole iron source when supplied with ferric enterobactin at approximately 10 microM, but growth stimulation was abolished when an omega (Omega) cassette was inserted within fetA or when tonB was insertionally interrupted [18].
  • Susceptibility testing with the mutants involved in the iron transport system indicated that the tonB mutant was resistant to BO-1341 [19].

Regulatory relationships of tonB

  • Mutations in tonB that suppressed the transport defect phenotype of these btuB mutations were isolated [20].

Other interactions of tonB

  • Finally, a strain carrying a mutant fhuA gene on the chromosome in which the linker insertion occurred after amino acid 82 showed a tonB phenotype [21].
  • Mutants devoid of all six iron-regulated outer membrane proteins were no more resistant to the probe compounds than fiu cir or tonB strains [1].
  • Analysis of the nucleotide sequence in the complementing DNA showed that it codes for three genes in the order of exbB-exbD-tonB [3].
  • This suggested involvement of both exbB and tolQ in tonB-dependent uptake [9].
  • The various tonB mutations differed markedly in their suppression activities on different btuB or cir mutations [20].


  1. Iron-regulated outer membrane proteins of Escherichia coli K-12 and mechanism of action of catechol-substituted cephalosporins. Curtis, N.A., Eisenstadt, R.L., East, S.J., Cornford, R.J., Walker, L.A., White, A.J. Antimicrob. Agents Chemother. (1988) [Pubmed]
  2. Cloning and sequencing of the Klebsiella pneumoniae tonB gene and characterization of Escherichia coli-K. pneumoniae TonB hybrid proteins. Bruske, A.K., Anton, M., Heller, K.J. Gene (1993) [Pubmed]
  3. Cloning and characterization of the exbB-exbD-tonB locus of Pasteurella haemolytica A1. Graham, M.R., Lo, R.Y. Gene (1997) [Pubmed]
  4. Neisseria meningitidis tonB, exbB, and exbD genes: Ton-dependent utilization of protein-bound iron in Neisseriae. Stojiljkovic, I., Srinivasan, N. J. Bacteriol. (1997) [Pubmed]
  5. Signal transfer through three compartments: transcription initiation of the Escherichia coli ferric citrate transport system from the cell surface. Härle, C., Kim, I., Angerer, A., Braun, V. EMBO J. (1995) [Pubmed]
  6. DNA sequence of the Escherichia coli tonB gene. Postle, K., Good, R.F. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  7. The beta-barrel domain of FhuADelta5-160 is sufficient for TonB-dependent FhuA activities of Escherichia coli. Braun, M., Killmann, H., Braun, V. Mol. Microbiol. (1999) [Pubmed]
  8. Characterization of the Vibrio cholerae outer membrane heme transport protein HutA: sequence of the gene, regulation of expression, and homology to the family of TonB-dependent proteins. Henderson, D.P., Payne, S.M. J. Bacteriol. (1994) [Pubmed]
  9. The structurally related exbB and tolQ genes are interchangeable in conferring tonB-dependent colicin, bacteriophage, and albomycin sensitivity. Braun, V. J. Bacteriol. (1989) [Pubmed]
  10. Mutagenic specificity of N-methyl-N'-nitro-N-nitrosoguanidine in the tonB gene on the chromosome of Escherichia coli recA+ and recA- cells. Wang, X., Kitamura, K., Yamamoto, K. Biochem. Biophys. Res. Commun. (1996) [Pubmed]
  11. Hydrogen peroxide-induced microsatellite instability in the Escherichia coli K-12 endogenous tonB gene. Yamamura, E., Nunoshiba, T., Nohmi, T., Yamamoto, K. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  12. Identification of two Shigella flexneri chromosomal loci involved in intercellular spreading. Hong, M., Gleason, Y., Wyckoff, E.E., Payne, S.M. Infect. Immun. (1998) [Pubmed]
  13. Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport. Gudmundsdottir, A., Bell, P.E., Lundrigan, M.D., Bradbeer, C., Kadner, R.J. J. Bacteriol. (1989) [Pubmed]
  14. X-ray-induced mutations in Escherichia coli K-12 strains with altered DNA polymerase I activities. Nagata, Y., Kawata, M., Komura, J., Ono, T., Yamamoto, K. Mutat. Res. (2003) [Pubmed]
  15. Transport across the outer membrane of Escherichia coli K12 via the FhuA receptor is regulated by the TonB protein of the cytoplasmic membrane. Schöffler, H., Braun, V. Mol. Gen. Genet. (1989) [Pubmed]
  16. Mode of action of GR69153, a novel catechol-substituted cephalosporin, and its interaction with the tonB-dependent iron transport system. Silley, P., Griffiths, J.W., Monsey, D., Harris, A.M. Antimicrob. Agents Chemother. (1990) [Pubmed]
  17. Involvement of ExbB and TonB in transport across the outer membrane of Escherichia coli: phenotypic complementation of exb mutants by overexpressed tonB and physical stabilization of TonB by ExbB. Fischer, E., Günter, K., Braun, V. J. Bacteriol. (1989) [Pubmed]
  18. Ferric enterobactin binding and utilization by Neisseria gonorrhoeae. Carson, S.D., Klebba, P.E., Newton, S.M., Sparling, P.F. J. Bacteriol. (1999) [Pubmed]
  19. Mode of action of BO-1341: transport pathway through the outer membrane of Escherichia coli. Sanada, M., Hashizume, T., Matsuda, K., Nakagawa, S., Tanaka, N. Drugs under experimental and clinical research. (1988) [Pubmed]
  20. Genetic suppression demonstrates interaction of TonB protein with outer membrane transport proteins in Escherichia coli. Bell, P.E., Nau, C.D., Brown, J.T., Konisky, J., Kadner, R.J. J. Bacteriol. (1990) [Pubmed]
  21. Insertion mutagenesis of the gene encoding the ferrichrome-iron receptor of Escherichia coli K-12. Carmel, G., Hellstern, D., Henning, D., Coulton, J.W. J. Bacteriol. (1990) [Pubmed]
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