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Gene Review

nfi  -  endonuclease V; deoxyinosine 3' endonuclease

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3990, JW5547, yjaF
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Disease relevance of nfi

  • The protein was purified to apparent homogeneity, and from its N-terminal sequence, its gene, nfi, was identified. nfi is immediately downstream of hemE, at kb 4208 (90.4 min) on the current chromosomal map of Escherichia coli K-12 [1].
  • However, neither the deficiency nor the overproduction of endonuclease V affected the growth of the single-stranded DNA phages M13 or phiX174 nor of Uracil-containing bacteriophage lambda [2].

High impact information on nfi


Chemical compound and disease context of nfi


Biological context of nfi


Associations of nfi with chemical compounds

  • Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982) [1].
  • An endonuclease V (nfi) mutation was tested for specific mutator effects on a battery of trp and lac mutant alleles [6].
  • However, these findings are consistent with a role for endonuclease V in the removal of deaminated guanine, i.e., xanthine, from DNA [6].
  • The nitrous acid-induced A:T-->G:C transitions were consistent with a role for endonuclease V in the repair of deaminated adenine residues [6].
  • During micro-aerobic growth, an nfi (Endo V) mutation enhanced the frequency of nitrate- and nitrite-induced A:T-->G:C and G:C-->A:T transition mutations, which are consistent with a defect in the removal of DNA hypoxanthine and xanthine, respectively [7].

Regulatory relationships of nfi


Other interactions of nfi


  1. nfi, the gene for endonuclease V in Escherichia coli K-12. Guo, G., Ding, Y., Weiss, B. J. Bacteriol. (1997) [Pubmed]
  2. Endonuclease V (nfi) mutant of Escherichia coli K-12. Guo, G., Weiss, B. J. Bacteriol. (1998) [Pubmed]
  3. Further characterization of Escherichia coli endonuclease V. Mechanism of recognition for deoxyinosine, deoxyuridine, and base mismatches in DNA. Yao, M., Kow, Y.W. J. Biol. Chem. (1997) [Pubmed]
  4. RdgB acts to avoid chromosome fragmentation in Escherichia coli. Bradshaw, J.S., Kuzminov, A. Mol. Microbiol. (2003) [Pubmed]
  5. Repair of deaminated bases in DNA. Kow, Y.W. Free Radic. Biol. Med. (2002) [Pubmed]
  6. Endonuclease V protects Escherichia coli against specific mutations caused by nitrous acid. Schouten, K.A., Weiss, B. Mutat. Res. (1999) [Pubmed]
  7. Endonuclease V of Escherichia coli prevents mutations from nitrosative deamination during nitrate/nitrite respiration. Weiss, B. Mutat. Res. (2001) [Pubmed]
  8. Incision at hypoxanthine residues in DNA by a mammalian homologue of the Escherichia coli antimutator enzyme endonuclease V. Moe, A., Ringvoll, J., Nordstrand, L.M., Eide, L., Bjørås, M., Seeberg, E., Rognes, T., Klungland, A. Nucleic Acids Res. (2003) [Pubmed]
  9. Genome-wide screening for genes whose deletions confer sensitivity to mutagenic purine base analogs in yeast. Stepchenkova, E.I., Kozmin, S.G., Alenin, V.V., Pavlov, Y.I. BMC Genet. (2005) [Pubmed]
  10. Repair system for noncanonical purines in Escherichia coli. Burgis, N.E., Brucker, J.J., Cunningham, R.P. J. Bacteriol. (2003) [Pubmed]
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