Disease relevance of ubiC

• These two enzymes are encoded by ubiC and ubiA, respectively, and have been reported to map near one another at 92 min on the E. coli chromosome [1].
• When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe-, Trp-, Tyr-) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid [2].

High impact information on ubiC

• These results establish Rv2949c as a chorismate pyruvate-lyase responsible for the direct conversion of chorismate to p-hydroxybenzoate and identify Rv2949c as the sole enzymatic source of p-hydroxybenzoic acid in M. tuberculosis [3].
• Metabolic engineering of the chloroplast genome using the Echerichia coli ubiC gene reveals that chorismate is a readily abundant plant precursor for p-hydroxybenzoic acid biosynthesis [4].
• Genetic engineering of plant secondary metabolism. Accumulation of 4-hydroxybenzoate glucosides as a result of the expression of the bacterial ubiC gene in tobacco [5].
• The sdgG suppressor is likely a mutation in one of three genes: ubiC, ubiA or yjbI [6].
• Genetic engineering of shikonin biosynthesis hairy root cultures of Lithospermum erythrorhizon transformed with the bacterial ubiC gene [7].

Chemical compound and disease context of ubiC

• The ubiC gene of Escherichia coli encodes chorismate pyruvatelyase, an enzyme that converts chorismate into 4-hydroxybenzoate (4HB) and is not normally present in plants [5].
• Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli [8].

Biological context of ubiC

• It is located directly downstream of ubiC in a typical operon structure [8].
• We have cloned the ubiCA gene cluster and determined the nucleotide sequence of ubiC and a portion of ubiA [1].
• Promoter sequences in the 5' flanking sequences of the ubiCA operon were localized by transcription and translation of active chorismate pyruvate-lyase in vitro from different PCR fragments [9].

Associations of ubiC with chemical compounds

• Although ubiquinone is implicated in antioxidant roles, ubiC expression was not elevated in response to hydrogen peroxide or the redox cycling agent, paraquat [10].
• Anaerobically utilised electron acceptors (nitrite, nitrate, fumarate) did not affect expression. ubiC expression appears to be negatively regulated by Fnr and IHF [10].
• Sequencing of the ubiC gene of the mutant strain AN244 revealed a G-->A transition resulting in a change from glutamic acid to lysine [9].
• 4HB produced by the CPL reaction was utilized for shikonin biosynthesis, as shown by in vivo inhibition of the native pathway to 4HB with 2-aminoindan-2-phosphonic acid (AIP), an inhibitor of phenylalanine ammonia-lyase [7].

References