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Gene Review

SETDB1  -  SET domain, bifurcated 1

Homo sapiens

Synonyms: ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, H3-K9-HMTase4, Histone H3-K9 methyltransferase 4, ...
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Psychiatry related information on SETDB1


High impact information on SETDB1


Biological context of SETDB1

  • Our data suggest a model in which H3-K9 methylation by SETDB1 is dependent on MBD1 and is heritably maintained through DNA replication to support the formation of stable heterochromatin at methylated DNA [3].
  • We mapped this gene, called SETDB1, to human chromosome 1q21 [5].
  • In vivo targeting of an enhanced green fluorescent protein-tagged HP1-lac repressor fusion to a lac operator-containing, gene-amplified chromosome region causes local condensation of the higher-order chromatin structure, recruitment of the histone methyltransferase SETDB1, and enhanced trimethylation of histone H3 lysine 9 [6].
  • Co-expression of SETDB1 and DNMT3A was essential for repression of reporter gene expression in a Gal4-based tethering assay and resulted in their recruitment to the artificial promoter [7].
  • We recently identified an ERG ( ets -related gene)-associated protein with a SET (suppressor of variegation, enhancer of zest and trithorax) domain (ESET) that was found to have the activity of a histone H3-specific methyltransferase [8].

Anatomical context of SETDB1

  • Furthermore, MCAF1 enhances transcriptional repression by MBD1 together with SETDB1, and exogenous expression of MCAF2 partly compensates for the repressive activity in MCAF1 knockdown HeLa cells [9].

Other interactions of SETDB1

  • This region is targeted by a large number of recurrent translocations, suggesting that like the SET domain protein MLL, mutant forms of SETDB1 may be associated with human neoplasias [5].
  • The interaction of SETDB1 with DNMT3A was further characterized and confirmed by in vivo and in vitro interaction studies [7].
  • Although sumoylated MBD1 binds to methylated DNA, it does not incorporate into a complex with SETDB1 and does not efficiently repress transcription of a target gene, p53BP2, in HeLa cells [10].

Analytical, diagnostic and therapeutic context of SETDB1


  1. ESET/SETDB1 gene expression and histone H3 (K9) trimethylation in Huntington's disease. Ryu, H., Lee, J., Hagerty, S.W., Soh, B.Y., McAlpin, S.E., Cormier, K.A., Smith, K.M., Ferrante, R.J. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  2. SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Schultz, D.C., Ayyanathan, K., Negorev, D., Maul, G.G., Rauscher, F.J. Genes Dev. (2002) [Pubmed]
  3. Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly. Sarraf, S.A., Stancheva, I. Mol. Cell (2004) [Pubmed]
  4. mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Wang, H., An, W., Cao, R., Xia, L., Erdjument-Bromage, H., Chatton, B., Tempst, P., Roeder, R.G., Zhang, Y. Mol. Cell (2003) [Pubmed]
  5. Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids. Harte, P.J., Wu, W., Carrasquillo, M.M., Matera, A.G. Cytogenet. Cell Genet. (1999) [Pubmed]
  6. In vivo HP1 targeting causes large-scale chromatin condensation and enhanced histone lysine methylation. Verschure, P.J., van der Kraan, I., de Leeuw, W., van der Vlag, J., Carpenter, A.E., Belmont, A.S., van Driel, R. Mol. Cell. Biol. (2005) [Pubmed]
  7. The histone methyltransferase SETDB1 and the DNA methyltransferase DNMT3A interact directly and localize to promoters silenced in cancer cells. Li, H., Rauch, T., Chen, Z.X., Szabó, P.E., Riggs, A.D., Pfeifer, G.P. J. Biol. Chem. (2006) [Pubmed]
  8. An ERG (ets-related gene)-associated histone methyltransferase interacts with histone deacetylases 1/2 and transcription co-repressors mSin3A/B. Yang, L., Mei, Q., Zielinska-Kwiatkowska, A., Matsui, Y., Blackburn, M.L., Benedetti, D., Krumm, A.A., Taborsky, G.J., Chansky, H.A. Biochem. J. (2003) [Pubmed]
  9. Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins. Ichimura, T., Watanabe, S., Sakamoto, Y., Aoto, T., Fujita, N., Nakao, M. J. Biol. Chem. (2005) [Pubmed]
  10. Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. Lyst, M.J., Nan, X., Stancheva, I. EMBO J. (2006) [Pubmed]
  11. Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor. Yang, L., Xia, L., Wu, D.Y., Wang, H., Chansky, H.A., Schubach, W.H., Hickstein, D.D., Zhang, Y. Oncogene (2002) [Pubmed]
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