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Gene Review

traA  -  conjugation protein

Agrobacterium fabrum str. C58

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Disease relevance of traA


High impact information on traA


Chemical compound and disease context of traA


Biological context of traA

  • The characteristics of the VirD1/VirD2-mediated cleavage reaction strongly resemble those observed with relaxosomes of IncP plasmids involved in initiation of transfer DNA replication during bacterial conjugation [9].
  • Expression of trlR inhibited octopine-induced conjugation of pTi15955 and pTiR10 by suppressing the TraR-mediated transcription of the tra and trb operons [10].
  • The effect of these vir gene mutations upon conjugation could be restored by the introduction of cosmids harboring wild-type copies of the corresponding disrupted vir genes into the mutant Agrobacterium strains [11].
  • The circular concept of the bacterial chromosome was based initially on experiments involving conjugation mapping and autoradiographic imaging of DNA [12].
  • Mpf/CP conjugation systems belong to the family of type IV secretion systems (T4SS), which also includes DNA-uptake and -release systems, as well as effector protein translocation systems of bacterial pathogens such as Agrobacterium tumefaciens (VirB/VirD4) and Helicobacter pylori (Cag) [13].

Anatomical context of traA


Associations of traA with chemical compounds


Other interactions of traA

  • The genes specifying production of octopine or nopaline by the tumor were transferred to recipient bacterial strains when the large plasmid associated with virulence was transferred by either conjugation or deoxyribonucleic acid-mediated transformation [20].

Analytical, diagnostic and therapeutic context of traA

  • By use of a sensitive, quantifiable assay termed transfer DNA immunoprecipitation (TrIP), we identify contacts between a DNA substrate (T-DNA) and 6 of 12 components of the VirB/D4 conjugation system of the phytopathogen Agrobacterium tumefaciens [21].
  • The Cercospora kikuchii cercosporin export gene, CFP, introduced into Beta vulgaris L. by conjugation with Rhizobium radiobacter, was stably maintained during vegetative propagation as verified by PCR using primers specific for the CFP gene [22].


  1. Agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and DNA conjugation. Stachel, S.E., Zambryski, P.C. Cell (1986) [Pubmed]
  2. Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines. Christie, P.J. Mol. Microbiol. (2001) [Pubmed]
  3. Bacterial secrets of secretion: EuroConference on the biology of type IV secretion processes. Baron, C., OCallaghan, D., Lanka, E. Mol. Microbiol. (2002) [Pubmed]
  4. Plant transformation by coinoculation with a disarmed Agrobacterium tumefaciens strain and an Escherichia coli strain carrying mobilizable transgenes. Pappas, K.M., Winans, S.C. Appl. Environ. Microbiol. (2003) [Pubmed]
  5. Mini-Mulac transposons with broad-host-range origins of conjugal transfer and replication designed for gene regulation studies in Rhizobiaceae. Ratet, P., Schell, J., de Bruijn, F.J. Gene (1988) [Pubmed]
  6. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Piper, K.R., Beck von Bodman, S., Farrand, S.K. Nature (1993) [Pubmed]
  7. Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Zhang, L., Murphy, P.J., Kerr, A., Tate, M.E. Nature (1993) [Pubmed]
  8. Localization of OccR-activated and TraR-activated promoters that express two ABC-type permeases and the traR gene of Ti plasmid pTiR10. Fuqua, C., Winans, S.C. Mol. Microbiol. (1996) [Pubmed]
  9. Initiation of Agrobacterium tumefaciens T-DNA processing. Purified proteins VirD1 and VirD2 catalyze site- and strand-specific cleavage of superhelical T-border DNA in vitro. Scheiffele, P., Pansegrau, W., Lanka, E. J. Biol. Chem. (1995) [Pubmed]
  10. Octopine-type Ti plasmids code for a mannopine-inducible dominant-negative allele of traR, the quorum-sensing activator that regulates Ti plasmid conjugal transfer. Oger, P., Kim, K.S., Sackett, R.L., Piper, K.R., Farrand, S.K. Mol. Microbiol. (1998) [Pubmed]
  11. vir genes influence conjugal transfer of the Ti plasmid of Agrobacterium tumefaciens. Gelvin, S.B., Habeck, L.L. J. Bacteriol. (1990) [Pubmed]
  12. The form of chromosomal DNA molecules in bacterial cells. Bendich, A.J. Biochimie (2001) [Pubmed]
  13. The mating pair formation system of conjugative plasmids-A versatile secretion machinery for transfer of proteins and DNA. Schröder, G., Lanka, E. Plasmid (2005) [Pubmed]
  14. Construction of a novel conjugative plasmid harboring a GFP reporter gene and its introduction into animal cells by transfection and trans-kingdom conjugation. Yoshida, K., Takegami, T., Katoh, A., Nishikawa, M., Nishida, T. Nucleic Acids Symp. Ser. (1997) [Pubmed]
  15. Characterization of conjugal transfer functions of Agrobacterium tumefaciens Ti plasmid pTiC58. von Bodman, S.B., McCutchan, J.E., Farrand, S.K. J. Bacteriol. (1989) [Pubmed]
  16. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. Fuqua, C., Winans, S.C. J. Bacteriol. (1996) [Pubmed]
  17. Agrobacterium rhizogenes GALLS protein substitutes for Agrobacterium tumefaciens single-stranded DNA-binding protein VirE2. Hodges, L.D., Cuperus, J., Ream, W. J. Bacteriol. (2004) [Pubmed]
  18. Expression of an antisense Datura stramonium S-adenosylmethionine decarboxylase cDNA in tobacco: changes in enzyme activity, putrescine-spermidine ratio, rhizogenic potential, and response to methyl jasmonate. Torrigiani, P., Scaramagli, S., Ziosi, V., Mayer, M., Biondi, S. J. Plant Physiol. (2005) [Pubmed]
  19. Modification of T-DNA of nopaline Ti plasmid by intermediary vector and utilization of agrocin 84 sensitivity as simple criterion of conjugation transfer of modified Ti plasmid. Vlasák, J., Hrouda, M., Bísková, R., Ondrej, M. Folia Microbiol. (Praha) (1986) [Pubmed]
  20. Octopine and nopaline metabolism in Agrobacterium tumefaciens and crown gall tumor cells: role of plasmid genes. Montoya, A.L., Chilton, M.D., Gordon, M.P., Sciaky, D., Nester, E.W. J. Bacteriol. (1977) [Pubmed]
  21. Definition of a bacterial type IV secretion pathway for a DNA substrate. Cascales, E., Christie, P.J. Science (2004) [Pubmed]
  22. Expression in sugar beet of the introduced cercosporin toxin export (CFP) gene from Cercospora kikuchii, the causative organism of purple seed stain in soybean. Kuykendall, L.D., Upchurch, R.G. Biotechnol. Lett. (2004) [Pubmed]
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