Disease relevance of XK 70-1

• Synergistic activities of fortimicin A and beta-lactam antibiotics against Pseudomonas aeruginosa [1].
• Fortimicin A had a bactericidal effect on Escherichia coli and Staphylococcus epidermidis and was found to inhibit protein synthesis in vivo [2].
• ODMF, fortimicin A, and gentamicin exhibited protective activity in mice infected with Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, S. aureus, or P. aeruginosa [3].
• Fortimicin A, a pseudodisaccharide aminoglycoside, was found to have broad-spectrum activity against most clinically important aerobic and facultatively anaerobic bacteria, except Pseudomonas aeruginosa, some other Pseudomonas species, and streptococci [4].
• A novel, highly efficient gene-cloning system in Micromonospora applied to the genetic analysis of fortimicin biosynthesis [5].

High impact information on XK 70-1

• The armA (aminoglycoside resistance methylase) gene, which confers resistance to 4,6-disubstituted deoxystreptamines and fortimicin, was initially found in Klebsiella pneumoniae BM4536 on IncL/M plasmid pIP1204 of ca. 90 kb which also encodes the extended-spectrum beta-lactamase CTX-M-3 [6].
• A49759 was two- to fourfold more active than fortimicin A against most species tested, but generally fourfold less active than amikacin against this population of Pseudomonas aeruginosa (85% inhibited at less than or equal to 16 microgram of amikacin per ml and 85% inhibited at less than or equal to 64 microgram of A49759 per ml) [7].
• ODMF and fortimicin A showed similar rapid bactericidal effects at multiples of the minimum inhibitory concentration and equivalent synergistic activity against enterococci when combined with penicillin G [3].
• We have developed a gene-cloning system in Micromonospora olivasterospora, a fortimicin A (astromicin) producer [5].
• Self cloning in Micromonospora olivasterospora of fms genes for fortimicin A (astromicin) biosynthesis [8].

Chemical compound and disease context of XK 70-1

• Acute and subacute toxicity studies with fortimicin A sulfate (Abbott-44747), a new aminoglycoside antibiotic [9].
• 1-Epidactimicin, a new aminoglycoside antibiotic converted from fortimicin B by a blocked mutant of istamycin-producing Streptomyces tenjimariensis [10].
• Enzymatic acetylation of fortimicin A and seldomycin factor 5 by aminoglycoside 3-acetyltransferase I: [AAC(3)-I] of E. coli KY8348 [11].

Biological context of XK 70-1

• The cloning of armA into a plasmid in Escherichia coli conferred to the new host high-level resistance to 4,6-disubstituted deoxystreptamines and fortimicin [12].
• DNA regions homologous to fms genes were found in Micromonospora sp. SF-2098 and Dactylosporangium matsuzakiense, both producers of fortimicin group antibiotics [13].
• The key intermediate, 1,2',6'-tri-N-benzyloxycarbonylfortimicin B, was prepared either directly from fortimicin B or by converting fortimicin A into 1,2',6',2''-tetra-N-benzyloxycarbonylfortimicin A (6a), followed by selective hydrolysis of the 4-N-(N-benzyloxycarbonyl)glycyl group of the latter [14].
• Simultaneous analysis of the serum and urinary pharmacokinetics of fortimicin A after intravenous administration to healthy subjects [15].
• One pattern exhibited broad substrate specificity including significant activity to fortimicin and was specific for Mycobacterium fortuitum strains, whereas the second pattern showed a much narrower substrate range and was observed for the other 3 environmental species [16].

Associations of XK 70-1 with other chemical compounds

• In general, the in vitro activity of fortimicin A was essentially the same as that of amikacin [17].
• The in vitro antimicrobial activity of O-demethylfortimicin A (ODMF), a derivative of fortimicin A, was compared with those of fortimicin A and gentamicin against a spectrum of 256 organisms [3].
• Dactimicin slowly degraded in vitro and in vivo to give fortimicin B as a main product which was accumulated in the kidney [18].
• Acid hydrolysis of fortimicin A indicated that it has one mole of glycine in its molecule while fortimicin B has not [19].

Gene context of XK 70-1

• Alkaline hydrolysis of dactimicin afforded 1-N-(N-formylglycyl)fortimicin B, fortimicin B, fortimicin A and an acyl migration product, 2'-N-(N-formylglycyl)fortimicin B [20].

References