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Chemical Compound Review

Acetolactate     2-acetyloxypropanoic acid

Synonyms: ACMC-209mjg, NSC-402126, AC1L2WIV, ACT03849, CTK1C1202, ...
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Disease relevance of AI3-03136


High impact information on AI3-03136


Chemical compound and disease context of AI3-03136


Biological context of AI3-03136


Anatomical context of AI3-03136

  • Although a major part of the acetolactate synthase synthesized by E. coli cells harbouring this vector was packaged into protein inclusion bodies, we used these recombinant E. coli cells to produce large quantities of the yeast enzyme [20].

Associations of AI3-03136 with other chemical compounds


Gene context of AI3-03136

  • Besides the expected ilvG gene product (59.3 kDa), purified acetolactate synthase contained a smaller subunit (9.7 kDa; designated here as the ilvM gene product) [4].
  • We isolated, in E. coli K12, new alleles of the ilvB locus, the structural gene for acetolactate synthase isoenzyme I, and showed them to map at or near the ilvB619 site [26].
  • Saccharomyces cerevisiae laboratory strains with an inactive ilv2 gene can not form acetolactate, while ilv5 strains, blocked in the subsequent step, leak acetolactate in high amounts [27].
  • Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification [28].
  • In the case of KARI inhibition, it is proposed that fermentation induction is due to an inhibition of ALS activity resulted from an increase in acetolactate concentration [29].

Analytical, diagnostic and therapeutic context of AI3-03136


  1. Glutamate 636 of the Escherichia coli pyruvate dehydrogenase-E1 participates in active center communication and behaves as an engineered acetolactate synthase with unusual stereoselectivity. Nemeria, N., Tittmann, K., Joseph, E., Zhou, L., Vazquez-Coll, M.B., Arjunan, P., Hübner, G., Furey, W., Jordan, F. J. Biol. Chem. (2005) [Pubmed]
  2. The crystal structures of Klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate. Pang, S.S., Duggleby, R.G., Schowen, R.L., Guddat, L.W. J. Biol. Chem. (2004) [Pubmed]
  3. Regulation by external pH and stationary growth phase of the acetolactate synthase from Synechocystis PCC6803. Maestri, O., Joset, F. Mol. Microbiol. (2000) [Pubmed]
  4. Purification and properties of Salmonella typhimurium acetolactate synthase isozyme II from Escherichia coli HB101/pDU9. Schloss, J.V., Van Dyk, D.E., Vasta, J.F., Kutny, R.M. Biochemistry (1985) [Pubmed]
  5. Degradative acetolactate synthase of Bacillus subtilis: purification and properties. Holtzclaw, W.D., Chapman, L.F. J. Bacteriol. (1975) [Pubmed]
  6. Identification and reconstitution of the yeast mitochondrial transporter for thiamine pyrophosphate. Marobbio, C.M., Vozza, A., Harding, M., Bisaccia, F., Palmieri, F., Walker, J.E. EMBO J. (2002) [Pubmed]
  7. Nucleotide sequence of the yeast ILV2 gene which encodes acetolactate synthase. Falco, S.C., Dumas, K.S., Livak, K.J. Nucleic Acids Res. (1985) [Pubmed]
  8. Molecular analysis of the acetolactate synthase gene of Chlamydomonas reinhardtii and development of a genetically engineered gene as a dominant selectable marker for genetic transformation. Kovar, J.L., Zhang, J., Funke, R.P., Weeks, D.P. Plant J. (2002) [Pubmed]
  9. Regulation of tobacco acetolactate synthase gene expression. Keeler, S.J., Sanders, P., Smith, J.K., Mazur, B.J. Plant Physiol. (1993) [Pubmed]
  10. Kinetics and mechanism of acetohydroxy acid synthase isozyme III from Escherichia coli. Gollop, N., Damri, B., Barak, Z., Chipman, D.M. Biochemistry (1989) [Pubmed]
  11. Acetohydroxy acid synthase activity from a mutation at ilvF in Escherichia coli K-12. Alexander-Caudle, C., Latinwo, L.M., Jackson, J.H. J. Bacteriol. (1990) [Pubmed]
  12. Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium. LaRossa, R.A., Van Dyk, T.K., Smulski, D.R. J. Bacteriol. (1987) [Pubmed]
  13. Metabolic interlock between the acetolactate synthase isoenzymes and lysine biosynthesis in Escherichia coli K-12. De Felice, M., Guardiola, J., Schreil, W., Levinthal, M., Iaccarino, M. Mol. Gen. Genet. (1977) [Pubmed]
  14. Effect of inactivation of nuo and ackA-pta on redistribution of metabolic fluxes in Escherichia coli. Yang, Y.T., Bennett, G.N., San, K.Y. Biotechnol. Bioeng. (1999) [Pubmed]
  15. Binding and activation of thiamin diphosphate in acetohydroxyacid synthase. Bar-Ilan, A., Balan, V., Tittmann, K., Golbik, R., Vyazmensky, M., Hübner, G., Barak, Z., Chipman, D.M. Biochemistry (2001) [Pubmed]
  16. Alkylation of acetohydroxyacid synthase I from Escherichia coli K-12 by 3-bromopyruvate: evidence for a single active site catalyzing acetolactate and acetohydroxybutyrate synthesis. Silverman, P.M., Eoyang, L. J. Bacteriol. (1987) [Pubmed]
  17. Cloning, sequencing and heterologous expression of a Klebsiella pneumoniae gene encoding an FAD-independent acetolactate synthase. Peng, H.L., Wang, P.Y., Wu, C.M., Hwang, D.C., Chang, H.Y. Gene (1992) [Pubmed]
  18. Transcriptional and Metabolic Responses of Bacillus subtilis to the Availability of Organic Acids: Transcription Regulation Is Important but Not Sufficient To Account for Metabolic Adaptation. Schilling, O., Frick, O., Herzberg, C., Ehrenreich, A., Heinzle, E., Wittmann, C., St??lke, J. Appl. Environ. Microbiol. (2007) [Pubmed]
  19. Protein trans-splicing to produce herbicide-resistant acetolactate synthase. Sun, L., Ghosh, I., Paulus, H., Xu, M.Q. Appl. Environ. Microbiol. (2001) [Pubmed]
  20. Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli. Poulsen, C., Stougaard, P. Eur. J. Biochem. (1989) [Pubmed]
  21. Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli. Wek, R.C., Hatfield, G.W. J. Mol. Biol. (1988) [Pubmed]
  22. An alternative pathway for the biosynthesis of isoprenoid compounds in bacteria. Pandian, S., Saengchjan, S., Raman, T.S. Biochem. J. (1981) [Pubmed]
  23. Sulfometuron methyl-sensitive and -resistant acetolactate synthases of the archaebacteria Methanococcus spp. Xing, R.Y., Whitman, W.B. J. Bacteriol. (1987) [Pubmed]
  24. ilvB-encoded acetolactate synthase is resistant to the herbicide sulfometuron methyl. LaRossa, R.A., Smulski, D.R. J. Bacteriol. (1984) [Pubmed]
  25. Branched-chain amino acid biosynthesis is essential for optimal growth of Streptococcus thermophilus in milk. Garault, P., Letort, C., Juillard, V., Monnet, V. Appl. Environ. Microbiol. (2000) [Pubmed]
  26. A new map location for the ilvB locus of Escherichia coli. Newman, T.C., Levinthal, M. Genetics (1980) [Pubmed]
  27. Towards diacetyl-less brewers' yeast. Influence of ilv2 and ilv5 mutations. Gjermansen, C., Nilsson-Tillgren, T., Petersen, J.G., Kielland-Brandt, M.C., Sigsgaard, P., Holmberg, S. J. Basic Microbiol. (1988) [Pubmed]
  28. Constricted flux through the branched-chain amino acid biosynthetic enzyme acetolactate synthase triggers elevated expression of genes regulated by rpoS and internal acidification. Van Dyk, T.K., Ayers, B.L., Morgan, R.W., Larossa, R.A. J. Bacteriol. (1998) [Pubmed]
  29. Fermentative metabolism is induced by inhibiting different enzymes of the branched-chain amino acid biosynthesis pathway in pea plants. Zabalza, A., González, E.M., Arrese-Igor, C., Royuela, M. J. Agric. Food Chem. (2005) [Pubmed]
  30. Purification, crystallization and preliminary X-ray crystallographic studies on acetolactate decarboxylase. Najmudin, S., Andersen, J.T., Patkar, S.A., Borchert, T.V., Crout, D.H., Fülöp, V. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
  31. A nondestructive method for peptide bond conjugation of antigenic haptens to a diphtheria toxoid carrier, exemplified by two antisera specific to acetolactate synthase. Marcussen, J., Poulsen, C. Anal. Biochem. (1991) [Pubmed]
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