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Chemical Compound Review

PEG 8000     2-[2-[2-[2-[2-[2-[2-[2-[2-[2- [2-[2-[2-[2...

Synonyms: CHEBI:44817, AC1NS1QD, PEU
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Disease relevance of PEG 8000

  • An Escherichia coli clone encoding a single cohesin domain of the cellulosome-integrating protein CipA from Clostridium thermocellum was constructed, and the corresponding polypeptide was purified, treated with papain, and crystallized from a PEG 8000 solution [1].
  • Carboxypeptidase 1 from the thermophilic eubacterium Thermus thermophilus (TthCP1, 58 kDa), a member of the M32 family of metallocarboxypeptidases, was crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitant [2].
  • In an attempt to examine the structural role of TRAP in the signal transduction pathway, TRAP from Staphylococcus aureus was overexpressed, purified and crystallized using PEG 8000 and 5% Jeffamine M600 (pH 7.0), as precipitants by hanging-drop vapour diffusion methods at 287 K [3].

High impact information on PEG 8000

  • We have crystallized Trypanosoma brucei PGK in the presence of the bisubstrate analog, adenylyl 1,1,5,5-tetrafluoropentane-1, 5-bisphosphonate, and solved the structure of this complex in two different crystal forms at 1.6 and 2.0 A resolution, obtained from PEG 8000 and ammonium phosphate solutions, respectively [4].
  • In addition to the pretreatment, 12.5% polyethyleneglycol with an average molecular weight of 8000 kDa (PEG 8000) was included in the transfection mixture containing the DNA complexes [5].
  • Crystals were grown in 0.1 M HEPES pH 7.5, 10% PEG 8000 and 8% ethylene glycol complemented with 9 mM 1-s-octyl-beta-D-thioglucoside or 0.1 M glycine [6].
  • The catalytic domain of death-associated protein kinase (DAPK) has been overexpressed, purified and crystallized using the sitting-drop vapour-diffusion method with PEG 8000 and magnesium acetate as precipitants [7].
  • Single well diffracting crystals are obtained after switching from the hanging-drop method to liquid-liquid diffusion in capillaries using PEG 8000 as precipitant [8].

Biological context of PEG 8000

  • Here, the GK domain was subcloned, expressed as an intein fusion protein, purified without the intein and then crystallized at room temperature by the hanging-drop vapour-diffusion method using PEG 8000 as a precipitant [9].
  • The supercoiling free energy of pUC19 DNA [2686 base pairs (bp)] was measured in various concentrations of PEG 8000 (polyethylene glycol; molecular weight 8000) by the topoisomer distribution method [10].
  • In contrast, PEG 8000-phosphate systems partitioned IBs more efficiently as a discrete sediment within the lower phase, whilst the majority of micronised debris remained in the interphase [11].

Anatomical context of PEG 8000

  • In the presence of or after exposure to PEG 3350 or PEG 8000, the protein could not be detected by Western blot analysis after SDS-PAGE suggesting that the protein failed to enter the gel even though other HeLa cell surface proteins were unaffected [12].

Associations of PEG 8000 with other chemical compounds


Gene context of PEG 8000

  • Free protein S antigen is measured by the same technique but after precipitation of the protein S-C4b-bp complex by PEG 8000 [18].
  • Crystals have been obtained using PEG 8000 as precipitant and cacodylate at pH 6.3 as buffer [19].
  • Crystals of the extracellular fragment of FcalphaRI/CD89 have been grown at 291 K using PEG 8000 as precipitant [20].
  • Trigonal crystals of native BSDL, with unit-cell parameters a = b = 90.0, c = 156.1 A, were obtained using 15-20%(w/v) PEG 8000 as precipitant [21].
  • Here, human UGDH (hUGDH) was purified and crystallized from a solution of 0.2 M ammonium sulfate, 0.1 M Na cacodylate, pH 6.5, and 21% PEG 8000 [22].

Analytical, diagnostic and therapeutic context of PEG 8000

  • Here, the crystallization of infestin 4 using the sitting-drop vapour-diffusion method with PEG 8000 as precipitant is described [23].
  • However, concentration-independent quantitative recovery of vincristine in saline solutions could be obtained by passivating the ultrafiltration devices with PEG-8000 and device drug adsorption was ameliorated for both agents by plasma [24].
  • We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials [25].


  1. Subcloning of a DNA fragment encoding a single cohesin domain of the Clostridium thermocellum cellulosome-integrating protein CipA: purification, crystallization, and preliminary diffraction analysis of the encoded polypeptide. Béguin, P., Raynaud, O., Chaveroche, M.K., Dridi, A., Alzari, P.M. Protein Sci. (1996) [Pubmed]
  2. Crystallization and preliminary X-ray analysis of carboxypeptidase 1 from Thermus thermophilus. Nagata, K., Tsutsui, S., Lee, W.C., Ito, K., Kamo, M., Inoue, Y., Tanokura, M. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  3. The target of RNAIII-activating protein (TRAP) from Staphylococcus aureus: purification, crystallization and preliminary X-ray analysis. Han, Y.H., Kim, Y.G., Kim, D.Y., Ha, S.C., Lokanath, N.K., Kim, K.K. Biochim. Biophys. Acta (2005) [Pubmed]
  4. A bisubstrate analog induces unexpected conformational changes in phosphoglycerate kinase from Trypanosoma brucei. Bernstein, B.E., Williams, D.M., Bressi, J.C., Kuhn, P., Gelb, M.H., Blackburn, G.M., Hol, W.G. J. Mol. Biol. (1998) [Pubmed]
  5. Transfection of epithelial cells is enhanced by combined treatment with mannitol and polyethyleneglycol. Düchler, M., Pengg, M., Brunner, S., Müller, M., Brem, G., Wagner, E. The journal of gene medicine. (2001) [Pubmed]
  6. Crystallization and preliminary X-ray crystallographic studies on a Kunitz-type potato serine protease inhibitor. Thomassen, E.A., Pouvreau, L., Gruppen, H., Abrahams, J.P. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  7. Crystallization and preliminary X-ray analysis of two inhibitor complexes of the catalytic domain of death-associated protein kinase. Yamakawa, A., Ogata, H., Morita, K., Shibata, N., Andou, N., Sanuki, H., Yamada, K., Hioki, T., Ishii, T., Higuchi, Y. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  8. Crystallization and X-ray diffraction data analysis of leukotriene A4 hydrolase from Saccharomyces cerevisiae. Andersson, B., Kull, F., Haeggström, J.Z., Thunnissen, M.M. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
  9. Crystallization and preliminary X-ray diffraction studies of the guanylate kinase-like domain of PSD-95 protein from rat. Kim, J.J., Rho, S.H., Im, Y.J., Kim, E.J., Eom, S.H. Acta Crystallogr. D Biol. Crystallogr. (2001) [Pubmed]
  10. Effect of polyethylene glycol on the supercoiling free energy of DNA. Naimushin, A.N., Quach, N., Fujimoto, B.S., Schurr, J.M. Biopolymers (2001) [Pubmed]
  11. Aqueous two-phase systems as an alternative process route for the fractionation of small inclusion bodies. Walker, S.G., Lyddiatt, A. J. Chromatogr. B Biomed. Sci. Appl. (1998) [Pubmed]
  12. Aqueous two-phase partition and detergent precipitation of a drug-responsive NADH oxidase from the HeLa cell surface. Morré, D.M., Sweeting, M., Morré, D.J. J. Chromatogr. B Biomed. Sci. Appl. (1998) [Pubmed]
  13. Cloning, purification and crystallization of full-length human annexin 2. Tran, J.T., Rosengarth, A., Luecke, H. Acta Crystallogr. D Biol. Crystallogr. (2002) [Pubmed]
  14. Structure of a new crystal form of human Hsp70 ATPase domain. Osipiuk, J., Walsh, M.A., Freeman, B.C., Morimoto, R.I., Joachimiak, A. Acta Crystallogr. D Biol. Crystallogr. (1999) [Pubmed]
  15. Thermal features of the bovine serum albumin unfolding by polyethylene glycols. Farruggia, B., Nerli, B., Di Nuci, H., Rigatusso, R., Picó, G. Int. J. Biol. Macromol. (1999) [Pubmed]
  16. Enhanced secretion and low temperature stabilization of a hyperthermostable and Ca2+-independent alpha-amylase of Geobacillus thermoleovorans by surfactants. Uma Maheswar Rao, J.L., Satyanarayana, T. Lett. Appl. Microbiol. (2003) [Pubmed]
  17. Crystallization of hepatocyte nuclear factor 1beta in complex with DNA. Lu, P., Li, Y., Gorman, A., Chi, Y.I. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. (2006) [Pubmed]
  18. Screening of protein S deficiency using a functional assay in patients with venous and arterial thrombosis. Wiesel, M.L., Charmantier, J.L., Freyssinet, J.M., Grunebaum, L., Schuhler, S., Cazenave, J.P. Thromb. Res. (1990) [Pubmed]
  19. Crystallization and preliminary X-ray diffraction studies of a D-lysine-based chiral PNA-DNA duplex. Menchise, V., De Simone, G., Corradini, R., Sforza, S., Sorrentino, N., Romanelli, A., Saviano, M., Pedone, C. Acta Crystallogr. D Biol. Crystallogr. (2002) [Pubmed]
  20. Crystallization and preliminary crystallographic analysis of the extracellular fragment of FcalphaRI/CD89. Yang, M., Xu, G., Li, S., Sun, L., Shi, N., Zeng, W., Pang, H., Zhang, W., Rao, Z. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
  21. Crystallization and preliminary X-ray analysis of native and recombinant human bile-salt dependent lipase: strategies for improvement of diffraction quality. Kingston, R.L., Baker, H.M., Loomes, K.M., Bläckberg, L., Hernell, O., Baker, E.N. Acta Crystallogr. D Biol. Crystallogr. (2000) [Pubmed]
  22. Expression, Purification, Crystallization, and Preliminary X-Ray Analysis of the Human UDP-Glucose Dehydrogenase. Huh, J.W., Robinson, R.C., Lee, H.S., Lee, J.I., Heo, Y.S., Kim, H.T., Lee, H.J., Cho, S.W., Choe, H. Protein Pept. Lett. (2006) [Pubmed]
  23. Crystallization, data collection and phasing of infestin 4, a factor XIIa inhibitor. Campos, I.T., Guimarães, B.G., Medrano, F.J., Tanaka, A.S., Barbosa, J.A. Acta Crystallogr. D Biol. Crystallogr. (2004) [Pubmed]
  24. Determination of free and liposome-associated doxorubicin and vincristine levels in plasma under equilibrium conditions employing ultrafiltration techniques. Mayer, L.D., St-Onge, G. Anal. Biochem. (1995) [Pubmed]
  25. Surface effects on PCR reactions in multichip microfluidic platforms. Panaro, N.J., Lou, X.J., Fortina, P., Kricka, L.J., Wilding, P. Biomedical microdevices. (2004) [Pubmed]
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