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Chemical Compound Review

Tween 20     2-[2-[3,4-bis(2- hydroxyethoxy)oxolan-2-yl]...

Synonyms: Polysorbate, CHEMBL371631, SureCN118527, CPD-3566, CHEBI:53423, ...
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Disease relevance of Tween 20

  • Vibrio cholerae bacteria of the serological variety O1 were consistently isolated from water samples by passing the water with added Tween 20 through columns packed with polystyrene beads coated with antibodies against the O1 antigenic determinants [1].
  • Four of the membrane proteins from Acholeplasma laidlawii that are soluble in the nonionic detergent Tween 20 have been purified by preparative electrophoretic techniques utilizing different supporting media [2].
  • Isolated HIV virions were disrupted by exposure to mild neutral detergents (Tween 20 and CHAPS) at concentrations from 0.25 to 2.0% [3].
  • The urea-induced equilibrium unfolding of T. thermophilus and E. coli IPMDHs at 27 degreesC was monitored by measuring the changes in far-UV CD, intrinsic fluorescence, anilinonaphthalenesulfonic acid (ANS) binding, and catalytic activity in the presence of nonionic detergent Tween 20 [4].
  • The assay is similar to that described for Pseudomonas aeruginosa (S. Vishwanath and R. Ramphal, Infect. Immun. 45:197-202, 1984), but notably 2% Tween 20 is used to desorb bacteria from the wells to quantitate bacterial binding [5].

Psychiatry related information on Tween 20


High impact information on Tween 20

  • To study the kinetics of the PMA-triggered NADPH oxidase in cell lysates, mixed detergents were used (deoxycholate and Tween 20) [7].
  • Unifoliolate leaves of 10-day-old greenhouse-grown seedlings were sprayed with 400 microliter per leaf of DFMO at various concentrations in 0.01% Tween 20 at pH 7.0 before or after inoculation with uredospores of Uromyces [8].
  • The culture supernatant solubilized by tween-20 exhibited a 95-kD and an 85-kD band on days 5 and 8, which corresponded to the intact and the truncated forms of TR, respectively [9].
  • These recoveries could be obtained when as little as 5,000 U of starting urinary Ep were processed because of the introduction of Tween 20 and SDS into buffers used at various stages of the purification procedure [10].
  • For the in vivo studies, female 4-5-week-old Apc(min) mice were randomized to two groups: a PC-SPES-treated group (n = 11) received 250 mg/kg/day (0.2 ml) PC-SPES via gastrointestinal gavage; and a control group (n = 10) received 0.2 ml of the vehicle solution (1.5% carboxymethylcellulose with 0.2% Tween 20) via gastrointestinal gavage [11].

Chemical compound and disease context of Tween 20


Biological context of Tween 20


Anatomical context of Tween 20


Associations of Tween 20 with other chemical compounds


Gene context of Tween 20

  • In contrast, buffers with Tween 20 caused no inhibition of BChE [31].
  • We therefore used octylglucoside, which does not affect darbufelone in this way, in place of Tween 20 in our PGHS buffers [32].
  • Native VLDL bound poorly to MTP, but its preincubation with Tween-20 resulted in significantly increased binding to MTP [33].
  • By addition of 1% Tween 20 to samples, the ELISA routinely estimated the total MT in samples of rat, mouse, and human liver and kidney at 88% of the value obtained by the silver-hem binding assay [34].
  • EGFR was also activated by mild surfactants, Tween-20 and Triton X-100, both in vitro (on immunopurified EGFR) and in intact living cells, thus indicating that EGFR is sensitive to amphiphilic molecules [35].

Analytical, diagnostic and therapeutic context of Tween 20

  • This protein was further purified from a Tween 20-insoluble-Triton X-100-soluble extract by salt fractionation, gel filtration chromatography, and hydrophobic interaction chromatography [27].
  • Enzyme kinetics using vesicles as well as optical titration using a micelle system with the detergent Tween 20 demonstrate that activation is correlated with the fraction of P450SCC in the high spin form [36].
  • The solubilized enzyme was purified 34-fold in the presence of 0.1% Tween 20 by four sequential chromatographies [37].
  • Immunoblotting of pollen proteins: Tween 20 mediates nonspecific binding of gammaglobulins to immunoblots of oak-pollen proteins [38].
  • Centrifugation of the Tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, 42, 39, 38, 34, 33.5, 33, 31, and 29 kDa [39].


  1. Isolation of O1 serovars of Vibrio cholerae from water by serologically specific method. Hranitzky, K.W., Larson, A.D., Ragsdale, D.W., Siebeling, R.J. Science (1980) [Pubmed]
  2. Purification of membrane proteins from Acholeplasma laidlawii by agarose suspension electrophoresis in Tween 20 and polyacrylamide and dextran gel electrophoresis in detergent-free media. Johansson, K.E., Blomqvist, I., Hjertén, S. J. Biol. Chem. (1975) [Pubmed]
  3. Atomic force microscopy investigation of human immunodeficiency virus (HIV) and HIV-infected lymphocytes. Kuznetsov, Y.G., Victoria, J.G., Robinson, W.E., McPherson, A. J. Virol. (2003) [Pubmed]
  4. Urea-induced unfolding and conformational stability of 3-isopropylmalate dehydrogenase from the Thermophile thermus thermophilus and its mesophilic counterpart from Escherichia coli. Motono, C., Yamagishi, A., Oshima, T. Biochemistry (1999) [Pubmed]
  5. Adhesion of nontypeable Haemophilus influenzae from blood and sputum to human tracheobronchial mucins and lactoferrin. Kubiet, M., Ramphal, R. Infect. Immun. (1995) [Pubmed]
  6. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Castro, C.A., Hogan, J.B., Benson, K.A., Shehata, C.W., Landauer, M.R. Pharmacol. Biochem. Behav. (1995) [Pubmed]
  7. Macrophage deactivation. Altered kinetic properties of superoxide-producing enzyme after exposure to tumor cell-conditioned medium. Tsunawaki, S., Nathan, C.F. J. Exp. Med. (1986) [Pubmed]
  8. Prevention of a plant disease by specific inhibition of fungal polyamine biosynthesis. Rajam, M.V., Weinstein, L.H., Galston, A.W. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  9. Expression and extracellular release of transferrin receptors during peripheral erythroid progenitor cell differentiation in liquid culture. Shintani, N., Kohgo, Y., Kato, J., Kondo, H., Fujikawa, K., Miyazaki, E., Niitsu, Y. Blood (1994) [Pubmed]
  10. Purification of human erythropoietin to homogeneity by a rapid five-step procedure. Krystal, G., Pankratz, H.R., Farber, N.M., Smart, J.E. Blood (1986) [Pubmed]
  11. PC-SPES inhibits colon cancer growth in vitro and in vivo. Huerta, S., Arteaga, J.R., Irwin, R.W., Ikezoe, T., Heber, D., Koeffler, H.P. Cancer Res. (2002) [Pubmed]
  12. Crossed immunoelectrophoresis, in the presence of tween 20 or sodium deoxycholate, of purified membrane proteins from Acholeplasma laidlawii. Johansson, K.E., Wróblewski, H. J. Bacteriol. (1978) [Pubmed]
  13. Effect of monolauryl succinate, lauryl alcohol and succinic acid on hemolysis and liver mitochondrial lysis in vitro in chicks. Ikumo, H. J. Nutr. (1980) [Pubmed]
  14. Bovine corneal stroma and epithelium reconstructed in vitro: characterisation and response to surfactants. Parnigotto, P.P., Bassani, V., Montesi, F., Conconi, M.T. Eye (London, England) (1998) [Pubmed]
  15. Highly specific inhibitory effect of three-component hybrid liposomes including sugar surfactants on the growth of glioma cells. Matsumoto, Y., Kato, T., Suzuki, H., Hirose, S., Naiki, Y., Hirashima, M., Ueoka, R. Bioorg. Med. Chem. Lett. (2000) [Pubmed]
  16. Effectiveness of spraying with tween 20 and lactic acid in decontaminating inoculated Escherichia coli O157:H7 and indigenous Escherichia coli biotype I on beef. Calicioglu, M., Kaspar, C.W., Buege, D.R., Luchansky, J.B. J. Food Prot. (2002) [Pubmed]
  17. Human aldolase B serum levels: a marker of liver injury. Asaka, M., Miyazaki, T., Hollinger, F.B., Alpert, E. Hepatology (1984) [Pubmed]
  18. Ultrasonic nebulization for efficient delivery of surfactant in a model of acute lung injury. Impact on gas exchange. Schermuly, R., Schmehl, T., Günther, A., Grimminger, F., Seeger, W., Walmrath, D. Am. J. Respir. Crit. Care Med. (1997) [Pubmed]
  19. In vitro cholesterol esterification in human serum. Yao, J.K., Dyck, P.J. Clin. Chem. (1977) [Pubmed]
  20. Studies on catalytic properties of purified high molecular weight pancreatic lipase. Yip, Y.K., Ramachandran, S., Wagle, S.R. Proc. Soc. Exp. Biol. Med. (1975) [Pubmed]
  21. Stimulation of dichlorofluorescin oxidation by capsaicin and analogues in RAW 264 monocyte/macrophages: lack of involvement of the vanilloid receptor. Garle, M.J., Knight, A., Downing, A.T., Jassi, K.L., Clothier, R.H., Fry, J.R. Biochem. Pharmacol. (2000) [Pubmed]
  22. Modulation of hepatic ferrochelatase activity by dietary manipulation of mitochondrial phospholipid fatty acyl groups. Kools, A.M., Straka, J.G., Hill, H.D., Whitmer, D.I., Holman, R.T., Bloomer, J.R. Hepatology (1989) [Pubmed]
  23. Intrathecal chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea encapsulated into hybrid liposomes for meningeal gliomatosis: an experimental study. Kitamura, I., Kochi, M., Matsumoto, Y., Ueoka, R., Kuratsu, J., Ushio, Y. Cancer Res. (1996) [Pubmed]
  24. Characterization of the mitochondrial carnitine palmitoyltransferase enzyme system. II. Use of detergents and antibodies. Woeltje, K.F., Kuwajima, M., Foster, D.W., McGarry, J.D. J. Biol. Chem. (1987) [Pubmed]
  25. The superoxide-generating respiratory burst oxidase of human neutrophil plasma membrane. Phosphatidylserine as an effector of the activated enzyme. Tamura, M., Tamura, T., Tyagi, S.R., Lambeth, J.D. J. Biol. Chem. (1988) [Pubmed]
  26. Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme. Ogino, N., Miyamoto, T., Yamamoto, S., Hayaishi, O. J. Biol. Chem. (1977) [Pubmed]
  27. Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli. Black, P.N., Said, B., Ghosn, C.R., Beach, J.V., Nunn, W.D. J. Biol. Chem. (1987) [Pubmed]
  28. pH modulation of ligand binding to adrenal mitochondrial cytochrome P-450scc. Jefcoate, C.R. J. Biol. Chem. (1982) [Pubmed]
  29. Characterization of a protein fatty acylesterase present in microsomal membranes of diverse origin. Berger, M., Schmidt, M.F. J. Biol. Chem. (1986) [Pubmed]
  30. False positivity in a cyto-ELISA for anti-endothelial cell antibodies caused by heterophile antibodies to bovine serum proteins. Revelen, R., Bordron, A., Dueymes, M., Youinou, P., Arvieux, J. Clin. Chem. (2000) [Pubmed]
  31. Abundant tissue butyrylcholinesterase and its possible function in the acetylcholinesterase knockout mouse. Li, B., Stribley, J.A., Ticu, A., Xie, W., Schopfer, L.M., Hammond, P., Brimijoin, S., Hinrichs, S.H., Lockridge, O. J. Neurochem. (2000) [Pubmed]
  32. Slow-binding inhibition of human prostaglandin endoperoxide synthase-2 with darbufelone, an isoform-selective antiinflammatory di-tert-butyl phenol. Johnson, A.R., Marletta, M.A., Dyer, R.D. Biochemistry (2001) [Pubmed]
  33. Apolipoprotein B binding to microsomal triglyceride transfer protein decreases with increases in length and lipidation: implications in lipoprotein biosynthesis. Hussain, M.M., Bakillah, A., Jamil, H. Biochemistry (1997) [Pubmed]
  34. Heterogeneity of antibodies to metallothionein isomers and development of a simple enzyme-linked immunosorbent assay. Chan, H.M., Pringle, G.A., Cherian, M.G. J. Biochem. Toxicol. (1992) [Pubmed]
  35. Activation of epithelial growth factor receptor pathway by unsaturated fatty acids. Vacaresse, N., Lajoie-Mazenc, I., Augé, N., Suc, I., Frisach, M.F., Salvayre, R., Nègre-Salvayre, A. Circ. Res. (1999) [Pubmed]
  36. Branched phosphatidylcholines stimulate activity of cytochrome P450SCC (CYP11A1) in phospholipid vesicles by enhancing cholesterol binding, membrane incorporation, and protein exchange. Kisselev, P., Wessel, R., Pisch, S., Bornscheuer, U., Schmid, R.D., Schwarz, D. J. Biol. Chem. (1998) [Pubmed]
  37. Dye-linked D-proline dehydrogenase from hyperthermophilic archaeon Pyrobaculum islandicum is a novel FAD-dependent amino acid dehydrogenase. Satomura, T., Kawakami, R., Sakuraba, H., Ohshima, T. J. Biol. Chem. (2002) [Pubmed]
  38. Immunoblotting of pollen proteins: Tween 20 mediates nonspecific binding of gammaglobulins to immunoblots of oak-pollen proteins. Loria, R.C., Prange, J.O., Wedner, H.J. J. Allergy Clin. Immunol. (1988) [Pubmed]
  39. Isolation of extracytoplasmic proteins from Serpulina hyodysenteriae B204 and molecular cloning of the flaB1 gene encoding a 38-kilodalton flagellar protein. Gabe, J.D., Chang, R.J., Slomiany, R., Andrews, W.H., McCaman, M.T. Infect. Immun. (1995) [Pubmed]
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