Disease relevance of NSC 298

• 2-Cyanocinnamic acid sensitization of L929 cells to killing by hyperthermia [1].

High impact information on NSC 298

• Purification of aldehyde dehydrogenase from rat liver mitochondria by alpha-cyanocinnamate affinity chromatography [2].
• The stimulatory effect of beta-hydroxybutyrate on pyruvate decarboxylation was abolished completely in the presence of 0.1 mM alpha-cyanocinnamate, an inhibitor of the pyruvate transporting system in the mitochondrial membrane [3].
• 1. N-Phenylmaleimide irreversibly inhibits pyruvate transport into rat heart and liver mitochondria to a much greater extent than does N-ethylmaleimide, iodoacetate or bromopyruvate. alpha-Cyanocinnamate protects the pyruvate transporter from attack by this thiol-blocking reagent [4].
• The extraction and reconstitution of the alpha-cyanocinnamate-sensitive pyruvate transporter from castor bean mitochondria [5].
• In our previous study on grass carp (Ctenopharyngodon idellus) liver ALDH, a significant amount of the ALDH activity did not adsorb on the alpha-cyanocinnamate Sepharose column which binds ALDH2 [6].

Biological context of NSC 298

• The pH affects only the affinity of the alpha-cyanocinnamate binding site without changing their total number [7].
• 3. This is corroborated by the reduction of glucose liberation in presence of alpha-cyanocinnamate, an inhibitor of gluconeogenesis [8].
• These properies suggested mediated transport, as did saturation kinetics and inhibition by several agents including pyruvate and alpha-cyanocinnamate [9].

Anatomical context of NSC 298

• 2. In both heart and liver mitochondria alpha-cyanocinnamate diminishes labelling by [3H]N-phenylmaleimide of a membrane protein of subunit mol.wt [4].
• The quantitative parameters of the binding of alpha-cyanocinnamate in submitochondrial particles have been determined [7].
• Characterization of the alpha-cyanocinnamate binding site in rat heart mitochondria and in submitochondrial particles [7].

Associations of NSC 298 with other chemical compounds

• Phenylglyoxal also inhibits the binding of alpha-cyanocinnamate to mitochondria [10].
• The results obtained indicate that arginine residues are involved in the mechanism of pyruvate translocation and of alpha-cyanocinnamate binding in rat-heart mitochondria [10].
• Oxoglutarate formed by omega-amidase activity is released as glutamate or oxidized by oxoglutarate dehydrogenase. alpha-Cyanocinnamate, the inhibitor of the monocarboxylate translocator in the mitochondrial membrane inhibited 4-methylthio-2-oxobutyrate-induced glutamine uptake and methionine release by about 30% [11].

References