Disease relevance of Mitochondria, Heart

• The role of phospholipase on the mechanism of doxorubicin-induced cardiomyopathy was investigated in the heart mitochondria of Wistar rats [1].
• Rat hepatoma cells, isolated rat hepatocytes and human polymorphonuclear leukocytes and lymphocytes stained positively with primary biliary cirrhosis sera, but not with normal sera or primary biliary cirrhosis sera preabsorbed with beef heart mitochondria [2].
• Tight divalent cation-binding sites of soluble adenosine triphosphatase (F1) from beef heart mitochondria and Escherichia coli [3].
• Protection by salvianolic acid A against adriamycin toxicity on rat heart mitochondria [4].
• The metals of the cytochrome c oxidase structures of the bovine heart mitochondrion (PDB code 1occ) and of the soil bacterium Paracoccus denitrificans (1arl) include a dicopper center (CuA), magnesium, two proximal hemes, a copper (CuB) atom, and a calcium [5].

High impact information on Mitochondria, Heart

• These studies were performed to determine whether oxidation of acetoacetate by isolated rat heart mitochondria results in a fall in intramitochondrial nonesterified CoA [CoASH] and whether increasing the available CoA improves contractile performance in hearts oxidizing acetoacetate [6].
• The oxidation of acetoacetate by isolated rat heart mitochondria resulted in depressed state 3 respiration as well as in a decrease in [CoASH] [6].
• During all phases of study, similar respiratory control rates and ADP:O ratios were found in myocardial mitochondria isolated from adriamycin-treated and control rabbits [7].
• Exposure of normal human sera to isolated human heart mitochondria at 37 degrees C resulted in the consumption of C1, C4, C2, and C3 without significant consumption of the terminal components of the complement system (C6 through C9) [8].
• Elegant studies with isolated heart mitochondria provided evidence that the creatine kinase reaction regulates the rate of oxidative phosphorylation [9].

Chemical compound and disease context of Mitochondria, Heart

• Phosphatidylethanolamine from bovine heart mitochondria, soya beans or Escherichia coli was equally effective in reconstituting respiratory control, suggesting that the specificity is referable to the head group and not to the fatty-acid moiety [10].
• Protective effect of urinary trypsin inhibitor on myocardial mitochondria during hemorrhagic shock and reperfusion [11].
• Effect of Escherichia coli endotoxin on adenine nucleotide translocation in canine heart mitochondria [12].
• The effects of chlorpromazine on various properties of the F1-ATPases from bovine heart mitochondria (MF1), the plasma membranes of Escherichia coli (EF1), and plasma membranes of the thermophilic bacterium PS3 (TF1) have been examined [13].
• Phosphorylation rates of canine heart mitochondria isolated after various periods of myocardial ischemia after cardioplegic arrest were correlated with the myocardial ATP-, lactate- and undissociated lactic acid content as well as with interstitial H+-concentration [14].

Biological context of Mitochondria, Heart

• Similarly, MEDICA 16 induced a saturable decrease in membrane potential, proton gradient, and proton motive force in isolated liver and heart mitochondria accompanied by an increase in mitochondrial respiration [15].
• Energy-linked nicotinamide nucleotide transhydrogenase. Kinetics and regulation of purified and reconstituted transhydrogenase from beef heart mitochondria [16].
• When intact rat heart mitochondria were pulsed with 150 nmol of CaCl2/mg of mitochondrial protein, only a marginal stimulation of the rate of oxygen consumption was observed [17].
• Endonuclease G activity, measured by a plasmid relaxation assay, did not show any age-associated change in liver, but there was a significant decrease from 6 to 23 months in heart mitochondria [18].
• We have investigated the influence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on bioenergetic functions of isolated heart-mitochondria [19].

Anatomical context of Mitochondria, Heart

• In heart mitochondria glycerophosphate has less effect on palmitylcarnitine formation because of glycerophosphate acyltransferase activity is lower and the carnitine palmityltransferase activity is higher in liver mitochondria [20].
• Electron spin resonance spectrometry with spin labeling and a trapping technique was applied to heart mitochondria obtained from treated and control animals, in order to examine the physical response of heart mitochondrial membrane affected by Adriamycin [21].
• Submitochondrial particles from beef heart mitochondria synthesize ADP-arsenate from ADP and arsenate when energized by succinate [22].
• The adenine nucleotide translocase was purified from bovine heart mitochondria and incorporated into membranes of phospholipid liposomes [23].
• 5. It is suggested that phosphorylation of sites additional to an inactivating site may inhibit the conversion of inactive complex into active complex by the phosphatase in heart mitochondria from alloxan-diabetic or 48h-starved rats or in mitochondria incubated for 6min with respiratory substrates [24].

Associations of Mitochondria, Heart with chemical compounds

• The deuterium magnetic resonance spectra of lipid-protein particles containing cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase, EC 1.9.3.1) isolated from beef heart mitochondria and the specifically deuterated lipid 1-(16,16,16-trideuteropalmitoyl)-2-palmitoleoyl phosphatidylcholine are presented [25].
• Phosphoenolpyruvate-stimulated calcium egress from heart mitochondria may, therefore, result from transport of this anion on the adenine nucleotide translocase [26].
• In contrast to liver, the tricarboxylate carrier system for citrate and phosphoenolpyruvate is rather low in heart mitochondria [26].
• In Experiment 2, rat heart mitochondria were incubated with ascorbate and ferrous sulfate [1].
• Bicarbonate did not activate pyruvate dehydrogenase in rat heart mitochondria [27].

Gene context of Mitochondria, Heart

• In the course of our studies on the structural diversity of the isoforms of cardiac fatty acid-binding proteins (cFABPs), a cardiac-type FABP from the matrix of bovine heart mitochondria was purified to homogeneity and obtained as a single 15-kDa protein with an isoelectric point of 4 [28].
• A 1:1 relationship for Mod-2 dosage and MOD-2 activity was found in heart mitochondria [29].
• UCP3 was present in brown-adipose-tissue mitochondria from warm-adapted rats but was undetectable in rat heart mitochondria [30].
• Here we identify the site of phosphorylation in NDUFA10 from bovine heart mitochondria by tandem mass spectrometry [31].
• Here, we report that human heart mitochondria contain frataxin species of increasing negative surface charge and molecular mass, ranging from monomer to polymers of >1 MDa [32].

Analytical, diagnostic and therapeutic context of Mitochondria, Heart

• Cytochrome c oxidase (ferrocytochrome c oxygen oxidoreductase, EC 1.9.3.1) was purified from beef heart mitochondria by affinity chromatography [33].
• 7. Using isolated heart mitochondria, it was demonstrated that pore opening could become CsA-insensitive under conditions of adenine nucleotide depletion and high matrix [Ca2+] such as may occur during the initial phase of reperfusion [34].
• 2. Titration of the rate of swelling with increasing concentrations of cyclosporin showed the number of cyclosporin-binding sites (+/- S.E.M.) in liver and heart mitochondria to be respectively 113.7 +/- 5.0 (n = 9) and 124.3 +/- 11.2 (n = 10) pmol/mg of protein, with a Ki of about 5 nM [35].
• Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria [36].
• Purified preparations of Complex I (NADH-ubiquinone oxidoreductase) from bovine heart mitochondria may be resolved into 26 polypeptides by two-dimensional analysis combining isoelectric focusing and polyacrylamide-gel electrophoresis in sodium dodecyl sulphate [37].

References