Disease relevance of Biotin-11-dutp

• M13 bacteriophage was labelled by primer extension with biotin-11-dUTP and was successfully used as probe to study human hypervariable minisatellites (DNA fingerprints) in Southern blots [1].
• The probe sequence to be used was first cloned into the M13 vector, and the minus strand of the template DNA was then synthesized with the Klenow fragment of E. coli DNA polymerase I in the presence of the biotinylated nucleotide, biotin-11-dUTP, as a label [2].

High impact information on Biotin-11-dutp

• HeLa cells in early S phase were encapsulated in agarose microbeads, permeabilized, and incubated with biotin-11-dUTP in a "physiological" buffer [3].
• The sites of DNA synthesis were detected by biotin-11-dUTP incorporation and compared with the distribution of PCNA by indirect immunofluorescence [4].
• Metabolic labelling studies with either biotin-11-dUTP or [32P]dCTP, revealed that nuclei lacking a lamina were unable to initiate DNA replication and that, although such nuclei could import proteins required for DNA replication (e.g. PCNA), these proteins were apparently not organized into replicon clusters [5].
• Firstly, proliferating fibroblasts growing in culture that had been synchronised at S-phase were microinjected with the thymidine analogue biotin-11-dUTP [4].
• The probe was prepared from single-stranded 25-30 base oligo d(T) and was biotinylated using the enzyme terminal deoxynucleotide transferase with biotin-11-dUTP and dTTP in the ratio 1:4 [6].

Biological context of Biotin-11-dutp

• Plasmids containing the B, F, G, H, and J fragments of VZV DNA were labeled by the nick translation method with biotin-11-dUTP as the dTTP analog [7].
• A biotinylated total genome M. gallisepticum DNA probe was constructed by labeling the DNA with biotin-11-dUTP in a standard nick-translation reaction [8].
• When repetitive sequence clones p1887 and K8.1a8 were labeled with digoxigenin and probe K8a.2G2 was labeled with biotin-11-dUTP and digoxigenin, the 3 chromosomes could be differentiated unambiguously when visualized with specific band-pass filters [9].
• PCR was performed in the presence of biotin-11-dUTP, and the product was used for in situ hybridization to detect the pancentromeric region of human chromosomes in metaphase spreads [10].
• Biotin-11-dUTP residues were added enzymatically, using terminal deoxynucleotidyl transferase, to the 3' terminus of a synthetic oligonucleotide prepared from the known nucleotide sequence for adenosine deaminase [11].

Anatomical context of Biotin-11-dutp

• That these observations reflected nicking specifically in myotube nuclei was determined by immunocytochemical localization of nicked sites after repair with a biotinylated nucleotide analog (biotin-11-dUTP) [12].
• After 2, 15, and 24 h of culture in 8 nM PMA, megakaryocytes were probed by in situ hybridization with biotin-11-dUTP-labeled cDNA probes for human c-myc, beta-actin, and coagulation cofactor V (FV) mRNA [13].

Associations of Biotin-11-dutp with other chemical compounds

• During the arrayed anchored multiplex PCR, 5[N-(N-biotinylaminocaproyl)-epsilon-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dUTP) was incorporated into the extended DNA chains in order to bind avidin alkaline phosphatase via avidin and biotin [14].

Gene context of Biotin-11-dutp

• A 998 bp fragment of plasmid pBR322, comprising part of the TEM-1 beta-lactamase gene, was labelled with biotin-11-dUTP for use as a DNA probe in a rapid non-isotopic spot hybridisation test [15].
• This was obtained by using nick-translated biotin-11-dUTP-labeled DNA probes and streptavidin-alkaline phosphatase conjugates for visualization of the hybridized signals [16].
• The probe pTZ-E4 was a fragment of the ovine beta-casein gene inserted in the plasmid pTZ18R and labeled with biotin-11-dUTP [17].
• The control ISPCR tests on the HCMV DNA positive specimens without adding either biotin-11-dUTP or Taq DNA polymerase or the primers for HCMV DNA showed negative results [18].

Analytical, diagnostic and therapeutic context of Biotin-11-dutp

• Changes in nuclear morphology have been characterized in the extract by phase-contrast microscopy and by fluorescence microscopy following pulse labelling with biotin-11-dUTP and staining with anti-lamin antibodies [19].
• The resulting PCR products were labelled by nick-translation with biotin-11-dUTP and used as probes for FISH [20].

References