Disease relevance of Spred1

• Using stably highly metastatic LM8 cells infected with the Spred1-Sendai virus vector, we demonstrated that Spred1 inhibits the metastasis of LM8 cells in nude mice [1].
• Spred-1 negatively regulates allergen-induced airway eosinophilia and hyperresponsiveness [2].

High impact information on Spred1

• Biochemical assays indicate that Spred-1 suppresses IL-5-dependent cell proliferation and ERK activation [2].
• These data indicate that Spred-1 negatively controls eosinophil numbers and functions by modulating IL-5 signaling in allergic asthma [2].
• Here, using Spred-1-deficient mice, we demonstrated that Spred-1 negatively regulates allergen-induced airway eosinophilia and hyperresponsiveness, without affecting helper T cell differentiation [2].
• We found that Spred-1 is highly expressed in interleukin-3 (IL-3)-dependent hematopoietic cell lines and bone marrow-derived mast cells [3].
• Augmentation of ERK activation and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow-derived mast cells [3].

Biological context of Spred1

• The Sprouty-related protein, Spred, inhibits cell motility, metastasis, and Rho-mediated actin reorganization [1].

Anatomical context of Spred1

• Wild-type Spred-1 inhibited colony formation of bone marrow cells in the presence of cytokines, whereas DeltaC-Spred-1 expression enhanced colony formation [3].
• In this study, we demonstrated that Spred-1 localized in cholesterol-rich membrane raft/caveola fractions and interacted with Cav-1 [4].
• The blastocysts hatched and grew on the endometrial epithelium as spherical coherent conglomerates of cells quite different from hatched blastocysts grown on the surface of a petri dish, in which the presumtive trophoblasts spred around the central mass [5].

Associations of Spred1 with chemical compounds

• Both Spred-1 and Spred-2 are membrane-associated substrates of receptor tyrosine kinases and they act as negative regulators of the Ras pathway upon growth factor stimulation [6].

Other interactions of Spred1

• To clarify the biological effect of Cav-1/Spred-1 interaction, we used hematopoietic cells that lacked expression of caveolins but expressed Spred-1 [4].

References