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Gene Review

Emp3  -  epithelial membrane protein 3

Mus musculus

Synonyms: EMP-3, Epithelial membrane protein 3, H-4, H4, HNMP-1, ...
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High impact information on Emp3

  • Here, we compared sequence and systematically mapped position of DNase I hypersensitive sites, acetylation status of histone H3/H4, and in vivo binding of transcription factors over approximately 120 kilobases flanking the human GATA1 gene and the corresponding region in mice [1].
  • Cell cycle regulation of a mouse histone H4 gene requires the H4 promoter [2].
  • The mouse histone H4 gene, when stably transformed into L cells on the PSV2gpt shuttle vector, is cell cycle regulated in parallel with the endogenous H4 genes [2].
  • The IFN regulatory factor-2 (IRF-2) oncoprotein controls the cell cycle-dependent expression of histone H4 genes during S phase and may function as a component of an E2F-independent mechanism to regulate cell growth [3].
  • Extending the study, we found that histones H1, H2A, H2B, H3, and H4 from calf thymus are all able to bind LPS [4].

Biological context of Emp3


Anatomical context of Emp3

  • Both also reacted with liver, H-4 recognizing antigen present on the hepatocyte, and H-13 recognizing antigen distributed along the sinusoid [9].
  • In the human fetal kidney, H-13 reacted with the mesangium, glomerular and tubular basement membrane and Bowman's capsule, and H-4 with the glomerular and tubular epithelial cells [9].

Associations of Emp3 with chemical compounds

  • Surprisingly, the widely used inhibitor trichostatin A (TSA) had little (cycling cells) or no (quiescent cells) effect on H4 acetylation in CD34+ UBC [10].
  • Bindings were specific, and affinities, determined by isothermal titration calorimetry, were (except for H4) higher than that of the LPS-binding antibiotic polymyxin B [4].
  • Immunoblot analysis of 2.0 M guanidine HCl extract after dissociation in sodium dodecyl sulfate and electrophoresis demonstrated binding of H-4 to a 125 kd polypeptide [9].
  • Affinity chromatography of rod domains of IF proteins on core histone-Sepharose 4B and of histones and their limit peptides on vimentin-Sepharose 4B has shown that the interactions involving fractions of histones H3 and H4 are extremely resistant to salt and can be dissociated only with arginine or salt under denaturing conditions [11].

Analytical, diagnostic and therapeutic context of Emp3


  1. Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 loci: implications for cis-element identification. Valverde-Garduno, V., Guyot, B., Anguita, E., Hamlett, I., Porcher, C., Vyas, P. Blood (2004) [Pubmed]
  2. Cell cycle regulation of a mouse histone H4 gene requires the H4 promoter. Seiler-Tuyns, A., Paterson, B.M. Mol. Cell. Biol. (1987) [Pubmed]
  3. Forced expression of the interferon regulatory factor 2 oncoprotein causes polyploidy and cell death in FDC-P1 myeloid hematopoietic progenitor cells. Xie, R.L., van Wijnen, A.J., van der Meijden, C.M., Stein, J.L., Stein, G.S. Cancer Res. (2002) [Pubmed]
  4. Histones: a novel class of lipopolysaccharide-binding molecules. Augusto, L.A., Decottignies, P., Synguelakis, M., Nicaise, M., Le Maréchal, P., Chaby, R. Biochemistry (2003) [Pubmed]
  5. Chromosomal mapping of Tmp (Emp1), Xmp (Emp2), and Ymp (Emp3), genes encoding membrane proteins related to Pmp22. Ben-Porath, I., Kozak, C.A., Benvenisty, N. Genomics (1998) [Pubmed]
  6. A single nucleotide polymorphism in the Emp3 gene defines the H4 minor histocompatibility antigen. Luedtke, B., Pooler, L.M., Choi, E.Y., Tranchita, A.M., Reinbold, C.J., Brown, A.C., Shaffer, D.J., Roopenian, D.C., Malarkannan, S. Immunogenetics (2003) [Pubmed]
  7. Histone H4 HDAC activity is necessary for expression of the PU.1 gene. Laribee, R.N., Klemsz, M.J. Biochim. Biophys. Acta (2005) [Pubmed]
  8. Ultrafine mapping of Dyscalc1 to an 80-kb chromosomal segment on chromosome 7 in mice susceptible for dystrophic calcification. Aherrahrou, Z., Doehring, L.C., Kaczmarek, P.M., Liptau, H., Ehlers, E.M., Pomarino, A., Wrobel, S., Götz, A., Mayer, B., Erdmann, J., Schunkert, H. Physiol. Genomics (2007) [Pubmed]
  9. Monoclonal antibodies to human glomerular antigens. Nakamura, T., Oite, T., Kazama, T., Suzuki, S., Orikasa, M., Arakawa, M., Shimizu, F. Virchows Archiv. A, Pathological anatomy and histopathology. (1988) [Pubmed]
  10. Human CD34+ hematopoietic progenitor cells hyperacetylate core histones in response to sodium butyrate, but not trichostatin A. Travers, H., Spotswood, H.T., Moss, P.A., Turner, B.M. Exp. Cell Res. (2002) [Pubmed]
  11. Interaction in vitro of non-epithelial intermediate filament proteins with histones. Traub, P., Perides, G., Kühn, S., Scherbarth, A. Z. Naturforsch., C, J. Biosci. (1987) [Pubmed]
  12. Histone acetylation induced by granulocyte colony-stimulating factor in a map kinase-dependent manner. Miyata, Y., Towatari, M., Maeda, T., Ozawa, Y., Saito, H. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
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