Disease relevance of Evx1

• An oligonucleotide segment containing this TRE/AP-1 site was found to confer Evx-1 inducibility on a simian virus 40 minimal promoter; mutation of the TRE/AP-1 site abolished this activity [1].

High impact information on Evx1

• Evidence for this parallel pathway emerged from an analysis of the induction of ventral progenitors that express the Dbx homeodomain proteins and of Evx1/2 (V0) and En1 (V1) neurons [2].
• In situ hybridization analysis demonstrates evx1 gene expression within the visceral endoderm after implantation and prior to gastrulation, at a time in which the mutant phenotype is first detected [3].
• In this study, we show that Evx1 V0 interneurons are locally projecting intersegmental commissural neurons [4].
• Evx1 is a postmitotic determinant of v0 interneuron identity in the spinal cord [4].
• In addition, Evx 1 and Evx 2 share extended amino acid conservation outside of the homeobox [5].

Biological context of Evx1

• During embryogenesis, the V0 and V1 classes of ventral interneurons are defined by expression of the homeodomain transcription factors Evx1/2 and En1, respectively [4].
• We have isolated the novel murine Sax-1 gene, a member of the NK-1 class of homeobox genes, and report its expression pattern in the developing central nervous system (CNS) in comparison to two other homeobox genes, Evx-1 and Pax-6 [6].
• Evx 1 is located 3.7 cM from the Hox 5 locus on mouse chromosome 2 [5].
• The Evx 1 protein consists of 416 amino acids as deduced from the longest open reading frame of Evx 1 cDNAs [5].
• Using the Drosophila even-skipped (eve) homeobox as a probe, we have isolated two murine genes, Evx 1 and Evx 2, from a genomic library [5].

Anatomical context of Evx1

• Finally, T and Evx1 display ectopic expression in cells not normally fated to ingress through the primitive streak [7].
• The Evx1- and Evx2-like central nervous system expression observed in these mice suggests that the long-range regulatory element(s) for the Hox cluster existed before the cluster duplication [8].
• From days 7 to 9 p.c. Evx 1 expression emerges at the posterior end of the embryo within the primitive ectoderm, and later in the mesoderm and neuroectoderm [5].
• From days 10 to 12.5 p.c. Evx 1 transcripts are restricted to specific cells within the neural tube and hindbrain along their entire lengths and coincides temporally, as well as spatially, with maturation of early forming interneurons, possibly commissural interneurons [5].
• We describe here the temporal and spatial pattern of expression of Evx-1, a murine homolog of the Drosophila even-skipped gene, in the developing limb bud [9].

Regulatory relationships of Evx1

• Conversely, misexpression of Evx1 represses En1, suggesting that Evx1 may suppress the V1 interneuron differentiation program [4].
• FGF-4 regulates expression of Evx-1 in the developing mouse limb [9].

Other interactions of Evx1

• Posteriorly, the expression domain overlapped with the Evx-1 expression in the streak, while anteriorly it was delimited by the Pax-6 signal in the neural tube [6].
• Results of wholemount in situ analysis show that the mRNA levels of Evx-1, Wnt-3a and Wnt-5a decrease in T/T embryos late in gastrulation, although earlier expression patterns are similar to control embryos [10].
• At all these stages, Evx-1 RNA is localized primarily to the posterior distal mesenchyme, in the region immediately underlying that portion of the ridge in which the Fgf-4 gene is expressed [9].
• Activation of the cytotactin promoter by the homeobox-containing gene Evx-1 [1].

References